Determination of High Oleic Type and Broomrape Resis tant Sunflower Hybrids By DNA Markers

Abstract

Sunflower is one of the mos t significant oilseed crops in the world. Sunflower lines with high oleic content have high

oxidative s tability of its oil. Orobanche cumana Wallr., which is a holoparasitic plant infecting the sunflower roots, is

one of the limiting factors for sunflower production especially in Eas tern Europe and Turkey. Screening for high oleic

acid content and broomrape resis tant sunflower genotypes by s tandard methods is time consuming and expensive.

Molecular markers associated with high oleic acid trait or broomrape resis tance are useful and rapid tool in order to

facilitate sunflower breeding program. In this s tudy, two markers were chosen; SSR marker and HO PCR specific

fragment for genotyping the 250 sunflower inbred lines for high oleic acid content. According to our results, high oleic

acid containing hybrids expressed a specific SSR band at 246 bp, and also HO PCR specific fragment at 870 bp. Also,

determination of broomrape resis tance for these 250 sunflower inbred lines was done by SCAR and SSR markers.

The analyzed SCAR markers (RTS28, RTS29, RTS40 and RTS41) and SSR markers (ORS1036 and ORS1040) were

linked to the Or5 gene [provides resis tance to all five races (A-E)]. According to SCAR and SSR analysis, the s tudied

sunflower inbred lines were resis tant to races A-E. These results allowed identification of sunflower hybrids for high

oleic acid traits and broomrape resis tance by DNA markers.

Keywords

• helianthus annuus,marker-assis ted selection,SCAR,SSR,oleic acid content,orobanche cumana

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