Development of BAC-End based simple sequence repeat (SSR) markers in apple

Elmira Ziya Motalebipour; Nergiz Coban; Mortaza Khodaeiaminjan; Murat Guney; Serif Ozongun; Nilgun Atay; Salih Kafkas

Development of BAC-End based simple sequence repeat (SSR) markers in apple

Year 2015, Volume: 1 Issue: 2, 84 - 92, 01.07.2015

Elmira Ziya Motalebipour Nergiz Coban Mortaza Khodaeiaminjan Murat Guney Serif Ozongun Nilgun Atay Salih Kafkas

Abstract

A genome-wide bacterial artificial chromosome (BAC) physical map of the apple, Malus domestica Borkh., has been recently developed. This study addresses development of SSR markers from the BAC-end sequences in apple. Previously designed 187 BAC-SSR primer pairs were subjected firstly to gradient-PCR for amplification and to determine their optimum annealing temperatures. Then, the amplified primer pairs were tested in two F1 segregating populations: ‘Kasel-37’ x ‘Delbarestivale’, and ‘Kasel-41’ X ‘Williams Pride’. The PCR reactions were performed in 12 F1 progenies and in the parents to determine segregation types of the primer pairs. There was no amplification in 20 SSR primer pairs, and 87 of them were monomorphic in the two populations. As a result, 80 of the primer pairs showed segregation in each of the two F1 populations

Keywords

SSR, apple, polymorphism, microsatellite

Year 2015, Volume: 1 Issue: 2, 84 - 92, 01.07.2015

Elmira Ziya Motalebipour Nergiz Coban Mortaza Khodaeiaminjan Murat Guney Serif Ozongun Nilgun Atay Salih Kafkas

Abstract

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Details

Other ID	JA35YM73ES
Journal Section	Articles
Authors	Elmira Ziya Motalebipour This is me Nergiz Coban This is me Mortaza Khodaeiaminjan This is me Murat Guney This is me Serif Ozongun This is me Nilgun Atay This is me Salih Kafkas This is me
Publication Date	July 1, 2015
Published in Issue	Year 2015 Volume: 1 Issue: 2

Cite

APA	Motalebipour, E. Z., Coban, N., Khodaeiaminjan, M., Guney, M., et al. (2015). Development of BAC-End based simple sequence repeat (SSR) markers in apple. Ekin Journal of Crop Breeding and Genetics, 1(2), 84-92.
AMA	Motalebipour EZ, Coban N, Khodaeiaminjan M, Guney M, Ozongun S, Atay N, Kafkas S. Development of BAC-End based simple sequence repeat (SSR) markers in apple. Ekin Journal. July 2015;1(2):84-92.
Chicago	Motalebipour, Elmira Ziya, Nergiz Coban, Mortaza Khodaeiaminjan, Murat Guney, Serif Ozongun, Nilgun Atay, and Salih Kafkas. “Development of BAC-End Based Simple Sequence Repeat (SSR) Markers in Apple”. Ekin Journal of Crop Breeding and Genetics 1, no. 2 (July 2015): 84-92.
EndNote	Motalebipour EZ, Coban N, Khodaeiaminjan M, Guney M, Ozongun S, Atay N, Kafkas S (July 1, 2015) Development of BAC-End based simple sequence repeat (SSR) markers in apple. Ekin Journal of Crop Breeding and Genetics 1 2 84–92.
IEEE	E. Z. Motalebipour, “Development of BAC-End based simple sequence repeat (SSR) markers in apple”, Ekin Journal, vol. 1, no. 2, pp. 84–92, 2015.
ISNAD	Motalebipour, Elmira Ziya et al. “Development of BAC-End Based Simple Sequence Repeat (SSR) Markers in Apple”. Ekin Journal of Crop Breeding and Genetics 1/2 (July 2015), 84-92.
JAMA	Motalebipour EZ, Coban N, Khodaeiaminjan M, Guney M, Ozongun S, Atay N, Kafkas S. Development of BAC-End based simple sequence repeat (SSR) markers in apple. Ekin Journal. 2015;1:84–92.
MLA	Motalebipour, Elmira Ziya et al. “Development of BAC-End Based Simple Sequence Repeat (SSR) Markers in Apple”. Ekin Journal of Crop Breeding and Genetics, vol. 1, no. 2, 2015, pp. 84-92.
Vancouver	Motalebipour EZ, Coban N, Khodaeiaminjan M, Guney M, Ozongun S, Atay N, Kafkas S. Development of BAC-End based simple sequence repeat (SSR) markers in apple. Ekin Journal. 2015;1(2):84-92.