Determination of High Oleic Type and Broomrape Resis tant Sunflower Hybrids By DNA Markers

Year 2018, Volume: 4 Issue: 1, 22 - 30, 30.01.2018

Behiye Banu Bılgen , Sheida Daneshvar Goksel Evcı Veli Pekcan Mehmet İbrahim Yılmaz Yalcin Kaya

Abstract

Sunflower is one of the mos t significant oilseed crops in the world. Sunflower lines with high oleic content have high

oxidative s tability of its oil. Orobanche cumana Wallr., which is a holoparasitic plant infecting the sunflower roots, is

one of the limiting factors for sunflower production especially in Eas tern Europe and Turkey. Screening for high oleic

acid content and broomrape resis tant sunflower genotypes by s tandard methods is time consuming and expensive.

Molecular markers associated with high oleic acid trait or broomrape resis tance are useful and rapid tool in order to

facilitate sunflower breeding program. In this s tudy, two markers were chosen; SSR marker and HO PCR specific

fragment for genotyping the 250 sunflower inbred lines for high oleic acid content. According to our results, high oleic

acid containing hybrids expressed a specific SSR band at 246 bp, and also HO PCR specific fragment at 870 bp. Also,

determination of broomrape resis tance for these 250 sunflower inbred lines was done by SCAR and SSR markers.

The analyzed SCAR markers (RTS28, RTS29, RTS40 and RTS41) and SSR markers (ORS1036 and ORS1040) were

linked to the Or5 gene [provides resis tance to all five races (A-E)]. According to SCAR and SSR analysis, the s tudied

sunflower inbred lines were resis tant to races A-E. These results allowed identification of sunflower hybrids for high

oleic acid traits and broomrape resis tance by DNA markers.

Keywords

helianthus annuus, marker-assis ted selection, SCAR, SSR, oleic acid content, orobanche cumana

References

• Alberio C, Izquierdo NG, Galella T, Zuil S, Reid R, Zambelli A and Aguirrezabal LA (2016).
• A new sunflower high oleic mutation confers s table oil grain fatty acid composition across environments. European Journal of Agronomy 73: 25-33.
• Amri M, Abbes Z, Ben Youssef S, Bouhadida M, Ben Salah H and Kharrat M (2012). Detection of the parasitic plant, Orobanche cumana on sunflower (Helianthus annuus L.) in Tunisia. Afr. J. Biotechnol. 11:4163-4167.
• Berville A, Lacombe S, Veillet S, Granier C, Leger S and Jouve P (2009). Method of selecting sunflower genotypes with high oleic acid content in seed oil, The Patent Cooperation Treaty (PCT), WO 2005/106022 A2.
• Cvejic S, Jocic S, Dimitrijevic A, Imerovski I, Miladinovic D, Jockovic M and Miklic V (2016).
• An EMS mutation altering oil quality in sunflower inbred line. In Kaya Y and Hasancebi S (eds) Proceedings of the 19thinternational sunflower conference. ISA, Edirne, p. 422-430.
• Dehmer KJ and Friedt W (1998). Development of molecular markers for high oleic acid content in sunflower (Helianthus annuus L.). Indus trial Crops and Products 7: 311-315.
• Dimitrijevic A, Imerovski I, Dragana M, Cvejic S, Jocic S, Zeremski T, Sakac Z (2017). Oleic acid variation and marker-assis ted detection of Pervenets mutation in high- and lowoleic sunflower cross. Crop Breed. Appl. Biotechnol. 17: 235-241.
• Ferfuia C, Turi M and Vannozzi GP (2012). Maternal effect on response of oleic acid content to temperature in high oleic sunflower. Helia 35: 19-28.
• Grandon NG, Moreno MV, Scorcione MC, Gieco JO, Alvarez D, Paniego N, Heinz R (2012).
• Characterization of sunflower inbred lines (Helianthus annuus L.) for high oleic acid content using SSR markers. p. 217.In: Proc. 18th Int. Sunfl. Conf., Mar del Plata, Argentina. Int. Sunfl. Assoc., Paris, France. Imerovski I, Dimitrijevic A, Miladinovic D, Dedic B, Jocic S and Miklic V (2012).
• Towards Orobanche resis tance in sunflower - SSR analysis of novel resis tance source. p. 1043- 1047. In: Proc. 18th Int. Sunfl. Conf., Mar del Plata, Argentina. Int. Sunfl. Assoc., Paris, France.
• Imerovski I, Dimitrijevic A, Miladinovic D, Dedic B, Jocic S, Kovacevic B and Obreht D (2013). Identification of PCR markers linked to different Or genes in sunflower. Plant Breeding 132:115- 120.
• Iuoras M, S tanciu D, CiucaM, Nas tase D and Geronzi F (2004). Preliminary s tudies related to the use of marker assis ted selection for resis tance to Orobanche cumana Wallr. in sunflower.
• Romanian Agricultural Research 21: 33-37. Kaya Y (2014). Current Situation of Sunflower Broomrape around the World. In Current Situation of Sunflower Broomrape around the World, Proceedings of the Third International Symposium on Broomrape (Orobanche spp.) in Sunflower, Cordoba, Spain, International Sunflower Association (ISA): Paris, France, pp 9-18.
• Lacombe S, Kaan F, Griveau Y and Berville A (2004). The Pervenets high oleic mutation: Methodological s tudies. Helia 40: 41-54.
• Leon AJ, Zambelli AD, Reid RJ, Morata MM, Kaspar M, Martinez-Force E, Garces R, Salas JJ and Venegas-Caleron M (2013). Isolated mutated nucleotide sequences that encode a modified oleate des taurase sunflower protein, modified protein, methods and uses. WIPO Patent WO/2013/004280, Jan 10.
• Louarn J, Boniface MC, Pouilly N, Velasco L, Perez-Vich B, Vincourt P and Munos S (2016).
• Sunflower Resis tance to Broomrape (Orobanche cumana) is Controlled by Specific QTLs for Different Parasitism S tages. Front. Plant Sci. 7: 590.
• Lu YH, Gagne G, Grezes-Besset B and Blanchard P (1999). Integration of a molecular linkage group containing broomrape resis tance gene Or5 into an RFLP map in sunflower. Genome 42:453-456.
• Lu YH, Melero-Vara JM, Garcia-Tejada JA and Blanchard P (2000). Development of SCAR markers linked to the gene Or5 conferring resis tance to broomrape (Orobanche cumana Wallr.) in sunflower. Theor. Appl. Genet. 100: 625-632. Marinkovic R, Jockovic M, Marjanovic-Jeromela A, Marisavljevic D and Sakac Z (2014). Resis tance evaluation of different inbreds and hybrids of sunflower to broomrape (Orobanche cumana Wallr.). Ratar. Povrt. 50(3): 53-59.
• Miladinovic D, Jocic S, Dedic B, Cvejic S, Dimitrijevic A, Imerovski I, Malidza G (2014). Current situation of sunflower broomrape in Serbia. In Current Situation of Sunflower Broomrape around the World, Proceedings of the 4(1):22-30, 2018 30 bitki ıslahçıları alt birliği w w w . b i s a b . o r g . t r Ekin Journal Third International Symposium on Broomrape (Orobanche spp.) in Sunflower, Cordoba, Spain, International Sunflower Association (ISA): Paris, France, pp 33-38. Molinero-Ruiz ML, R. Garcıa-Ruiz, JM Melero-Vara and Dominguez J (2009). Orobanche cumana race F: performance of resis tant sunflower hybrids and aggressiveness of populations of the parasitic weed. Weed Res. 49: 469-478. Nagarathna TK, Shadakshari YG and Ramanappa TM (2011). Molecular analysis of sunflower (Helianthus annuus L.) genotypes for high oleic acid using microsatellite markers. Helia 34: 63-68. Osorio J, Fernández-Martinez JM, Mancha M and Garces R (1995). Mutant sunflower with high concentration in saturated fatty acid in the oil. Crop Science 35: 739-742. Pacureanu-Joita M, Ciuca M and Sava E (2012).
• Broomrape (Orobanche cumana Wallr.), the mos t important parasite in sunflower: virulence, race dis tribution, hos t resis tance. Proc. p. 1052- 1057. In: Proc. 18th Int. Sunflower Conf., Mar del Plata, Argentina. Int. Sunflower Assoc., Paris, France.
• Perez-Vich B, Velasco L, Rich PJ and Ejeta G (2013). Marker-assis ted and physiology-based breeding for resis tance to root parasitic Orobanchaceae. p. 369-392. In: D.M. Joel, J. Gressel, L. and J. Musselman (eds.), Parasitic Orobanchaceae. Springer, New York, USA.
• Premnath A, Narayana M, Ramakrishnan C, Kuppusamy S and Chockalingam V (2016).
• Mapping quantitative trait loci controlling oil content, oleic acid and linoleic acid content in sunflower (Helianthus annuus L.).
• Mol Breeding (2016) 36:106 Schuppert GF, Tang S, Slabaugh MB and Knapp SJ (2006).
• The sunflower high-oleic mutant Ol carries variable tandem repeats of FAD2- 1, a seed-specific oleoyl-phosphatidyl choline desaturase.
• Molecular Breeding 17: 241-256. Singchai A, Muangsan N and Machikowa T (2013).
• Evaluation of SSR markers associated with high oleic acid in sunflower. International Journal of Biological, Food, Veterinary and Agricultural Engineering, 7: 631-634. Soldatov KI (1976).
• Chemical mutagenesis in sunflower breeding. Proc. 7th International Sunflower Conference, 27 June - 3 July, Krasnodar, Russia, Pp. 352-357.
• Tang S, Heesacker A, Kishore VK, Fernandez A, Sadik ES, Cole G and Knapp SJ (2003).
• Genetic mapping of the Or5 gene for resis tance to Orobanche race E in sunflower. Crop. Sci. 43:1021-1028.
• Velasco L, Perez-Vich B and Fernández-Martínez JM (2008). A new sunflower mutant with increased levels of palmitic acid in seed oil. Helia 31: 55-60.
• Vannozzi GP (2006). The perspectives of use of high oleic sunflower for oleochemis try and energy raws. Helia 29: 1-24.
• Varshney RK, Graner A and Sorrels ME (2005). Genic microsatellite markers in plants: features and applications. Trends Biotechnol 23(1): 48-55.