Research Article
BibTex RIS Cite

Improving Grain Quality in Pulses: S trategies to Reduce Raffinose Family Oligosaccharides in Seeds

Year 2018, Volume: 4 Issue: 1, 70 - 88, 30.01.2018

Abstract

In human diet, pulses are an excellent source of carbohydrates, proteins, dietary fibers, vitamins, minerals and other

bioactive compounds. However, the presence of high concentration of raffinose family oligosaccharides (RFO) limits

their consumption and acceptance worldwide especially in developed countries. Humans and mono-gas tric animals cannot

diges t RFO but are fermented by large intes tinal microflora that produces carbon dioxide, hydrogen and methane causing

flatulence and s tomach discomfort. Hence, it is imperative to develop s trategies to reduce RFO concentration in pulses

to promote their consumption in human diet around the world.RFO are sucrosyl galactosidessynthesized during the later

s tages of seed development. RFO accumulation in seeds is affected by crop species, genotype and growing environment.

Genetic s trategies have been used to reduce the accumulation of RFO in pulses. Several pos t-harves t processing methods

have also been used to reduce RFO concentration in pulses used for human consumption.

References

  • Aguilera Y, Martín-Cabrejas MA, Benítez V, Mollá E, López-Andréu FJ and Es teban RM (2009). Changes in carbohydrate fraction during dehydration process of common legumes. J Food Compos Anal 22: 678-683.
  • Alajaji SA and El-Adawy TA (2006). Nutritional composition of chickpea (Cicer arietinum L.) as affected by microwave cooking and other traditional cooking methods. J Food Compos Anal 19: 806-812.
  • Al-Kaisey MT, Alwan AKH, Mohammad MH and Saeed AH (2003). Effect of gamma irradiation on antinutritional factors in broad beans. Radiat Phys Chem 67: 493-496.
  • Allan SM and Hitz WD (2000). Plant raffinose synthase homologs. International Patent Publication WO 00/24915, PCT/US99/24923. Andersen KE, Bjergegaard C, Moller P, Sorensen JC and Sorensen H (2005). Compositional variations for α-galactosides in different species of leguminosae, brassicaceae, and barley: a chemotaxonomic s tudy based on chemometrics and high-performance capillary electrophoresis. J Agric Food Chem 53: 5809-5817.
  • Andrews M and Andrews ME (2017). Specificity in Legume-Rhizobia symbioses. Int J Mol Sci 18: 705.
  • Anisha GS and Prema P (2008). Reduction of nondiges tible oligosaccharides in horse gram and green gram flours using crude α-galactosidase from S treptomyces griseoloalbus. Food Chem 106: 1175-1179.
  • Aranda P, Dos talova J, Frias J, Lopez-Jurado M, Kozłowska H, Pokorny J, Urbano G, Vidal- Valverde C and Zduńczyk Z (2001). Nutrition. In: Carbohydrates in grain legume seeds. Improving nutritional quality and agronomic characteris tics, Hedley CL (eds.), CAB Int. Wallingford, pp: 61-87.
  • Avigad G and Dey PM (1997). Carbohydrate metabolism: S torage carbohydrates. In: Plant biochemis try, Dey PM and Harbourne J (eds.), Academic Press, San Diego, pp: 143-204.
  • Bachmann M and Keller F (1995). Metabolism of the raffinose family oligosaccharides in leaves of Ajuga reptans L. Inter- and intracellular compartmentation. Plant Physiol 109: 991-998.
  • Bachmann M, Matile P and Keller F (1994). Metabolism of the raffinose family oligosaccharides in leaves of Ajuga reptans L. Cold acclimation, translocation, and sink to source transition: Discovery of a chain elongation enzyme. Plant Physiol 105: 1335-1345.
  • Bilyeu KD and Wiebold WJ (2016). Environmental s tability of seed carbohydrate profiles in soybeans containing different alleles of the raffinose synthase 2 (RS2) gene. J Agri Food Chem 64: 1071–1078.
  • Blӧchl A, Peterbauer T and Richter A (2007). Inhibition of raffinose oligosaccharide breakdown delays germination of pea seeds. J Plant Physiol 164: 1093-1096.
  • Bock C, Ray H and Georges F (2009). Down-regulation of galactinol synthesis in oilseed Brassica napus leads to significant reduction of antinutritional oligosaccharides.Botany 87: 597-603.
  • Brasil APR, Tavares de Rezende S, Gouveia Pelúzio M and Guimarães VM (2010). Removal of oligosaccharides in soybean flour and nutritional effects in rats. Food Chem 118: 251-255.
  • Cao Y, Yuan T, Shi P, Luo H, Li N, Meng K, Bai Y, Yang P, Zhou Z, Zhang Z and Yao B (2010). Properties of a novel α-galactosidase from S treptomyces sp. S27 and its potential for soybean processing. Enzy Micro Techn 47: 305-312.
  • Cas tillo EM, De Lumen BO, Reyes PS and De Lumen HZ (1990). Raffinose synthase and galactinol synthase in developing seeds and leaves of legumes. J Agric Food Chem 38: 351-355.
  • Chibbar RN, Ambigaipalan P and Hoover R (2010). Molecular diversity in pulse seed s tarch and complex carbohydrates and its role in human nutrition and health. Cereal Chem 87: 342- 352.
  • Chilomer K, Zaleska K, Ciesiołka D, Gulewicz P, Frankiewicz A and Gulewicz K (2010). Changes in the alkaloid, α-galactoside and protein fraction content during germination of different lupin spcies. Acta Soc Bot Pol 79: 11-20.
  • Cicek MS, Chen P, Saghai Maroof MA and Buss GR (2006). Interrelationships among agronomic and seed quality traits in an interspecific soybean recombinant inbred population. Crop Sci 46: 1253-1259.
  • Clarke EJ and Wiseman J (2000). Developments in plant breeding for improved nutritional quality of soya beans II. Anti-nutritional factors.J Agri Sci Camb 134: 125-136. 4(1):70-88, 2018
  • Coon CN, Leske KL, Akavanichan O and Cheng TK (1990). Effect of oligosaccharides-free soybean meal on true metabolizable energy and fibre diges tion in adult roos ters. Poult Sci 69: 787-793.
  • Das A and Parida SK (2014). Advances in biotechnological applications in three important food legumes.Plant Biotechnol Rep 8:83–99.
  • De Faria SM, Lewis GP, Sprent JI and Sutherland JM (1989). Occurrence of nodulation in the leguminosae. New Phytol 111: 607-619.
  • Devindra, S, Rao JS, Krishnaswamya P and Bhaskarb V (2011). Reduction of α-galactoside content in red gram (Cajanus cajan L.) upon germination followed by heat treatment. J Sci Food Agric 91: 1829-1835.
  • Dey PM and Campillo ED (1984). Biochemis try of the multiple forms of glycosidases in plants. Adv Enzymol Relat Areas Mol Biol 56: 141-249.
  • Dharmasena MN and Mathew CD (2002). Reducing flatulence factors in soymilk using coconut kernel α-galactosidase immobilised hydrophobic gels. J Natn Sci Foundation 30(3&4): 161-169.
  • Dierking EC and Bilyeu KD (2008). Association of a soybean raffinose synthase gene with low raffinose and s tachyose seed phenotype. Plant Genome 1: 135–145.
  • Dierking EC and Bilyeu KD (2009). New sources of soybean seed meal and oil composition traits identified through TILLING. BMC Plant Biol 9:89.
  • Dilis V and Trichopoulou A (2009). Nutritional and health properties of pulses. Mediterr. J Nutr Metab 1: 149-157.
  • Dos Santos TB, Budzinski IG, Marur CJ, Petkowicz CL, Pereira LF and Vieira LG (2011). Expression of three galactinol synthase isoforms in Coffea arabica L. and accumulation of raffinose and s tachyose in response to abiotic s tresses. Plant Physiol Biochem 49: 441-448.
  • Downie B, Gurusinghe S, Dahal P, Thacker RR, Snyder JC, Nonogaki H, Yim K, Fukanaga K, Alvarado V and Bradford KJ (2003). Expression of a galactinol synthase gene in tomato seeds is upregulated before maturation desiccation and again after imbibition whenever radicle protrusion is prevented. Plant Physiol 131: 1347-59.
  • Egbert A, Keller F and Peters S (2013). Abiotic s tressinduced accumulation of raffinose in Arabidopsis leaves is mediated by a single raffinose synthase (RS5, At5g40390). BMC Plant Biol 13: 218.
  • El-Adawy TA (2002). Nutritional composition and antinutritional factors of chickpeas (Cicer arietinum L.) undergoing different cooking methods and germination. Plant Foods Hum Nutr 57: 83-97.
  • ElSayed AI, Rafudeen MS and Golldack D (2014). Physiological aspects of raffinose family oligosaccharides in plants: protection agains t abiotic s tress. Plant Biol 16: 1–8.
  • Falkoski DL, Guimarães VM, Callegari CM, Reis AP, Barros EG and Rezende S (2006). Processing of soybean products by semipurified plant and microbial α-galactosidases.J Agric Food Chem 54: 10184-10190.
  • FAOSTAT (2016). http://www.fao.org/faos tat/en/#data/ QC (accessed on April 26, 2017).
  • Frias J, Bakhash A, Jones DA, Arthur AE, Vidal- Valverde C, Rhodes MJC and Hedley CL (1999). Genetic analysis of the raffinose oligosaccharide pathway in lentil seeds. J Exp Bot 50: 469-476.
  • Frias J, Giacomino S, Peñas E, Pellegrino N, Ferreyra N, Apro V, Olivera-Carrión M and Vidal-Valverde C (2011). Assessment of the nutritional quality of raw and extruded Pisum sativum L. var. Laguna seeds. LWT – Food Sci. Technol 44: 1303-1308.
  • Frias J, Vidal-Valverde C, Sotomayor C, Diaz-Pollan C and Urbano G (2000). Influence of processing on available carbohydrate content and antinutritional factors of chickpeas. Eur Food Res Technol 210: 340-345.
  • Gangola MP, Behl RK, Chibbar RN (2014a) S trategies to improve seed quality of pulse crops to ensure global food and nutrition security. In: Cutting edge science and technologies for food, environment and health, Behl RK, Asif AR and Merbach W (eds.), Agrobios (International), Jodhpur, India, pp: 7-21.
  • Gangola MP, Jaiswal S, Kannan U, Gaur PM, Båga M and Chibbar RN (2016). Galactinol synthase enzyme activity influences raffinose family oligosaccharides (RFO) accumulation in developing chickpea (Cicer arietinum L.) seeds. Phytochem 125: 88-98.
  • Gangola MP, Jaiswal S, Khedikar YP and Chibbar RN (2014b) A reliable and rapid method for soluble sugars and RFO analysis in chickpea using HPAEC–PAD and its comparison with HPLC–RI. Food Chem 154: 127–133.
  • Gangola MP, Khedikar YP, Gaur PM, Båga M and Chibbar RN (2012). Variation in important seed cons tituents among various chickpea genotypes. Qual Assur Saf Crop 4: 136–158.
  • Gangola MP, Khedikar YP, Gaur PM, Båga M and Chibbar RN (2013). Genotype and growing environment interaction shows a positive correlation between subs trates of raffinose family oligosaccharides (RFO) biosynthesis and their accumulation in chickpea (Cicer arietinum L.) seeds. J Agric Food Chem 61: 4943–4952.
  • Gil L, Ben-Ari J, Turgeon R and Wolf S (2012). Effect of CMV infection and high temperatures on the enzymes involved in raffinose family oligosaccharide biosynthesis in melon plants. J Plant Physiol 169: 965-970.
  • Girigowda K, Prashanth SJ and Mulimani VH (2005). Oligosaccharins of black gram (Vigna mungo L.) as affected by processing methods. Plant Foods Hum Nutr 60: 173-80.
  • Glencross BD, Boujard T and Kaushik SJ (2003). Influence of oligosaccharides on the diges tibility of lupin meals when fed to rainbow trout Oncorhynchus mykiss. Aquaculture 219: 703-713. Gote M, Umalkar H, Khan I and Khire J (2004).
  • Thermos table α-galactosidase from Bacillus s tearothermophilus (NCIM 5146) and its application in the removal of flatulence causing factors from soymilk. Process Biochem 39: 1723- 1729.
  • Granito M, Frias J, Doblado R, Guerra M, Champ M and Vidal–Valverde C (2002). Nutritional improvement of beans (Phaseolus vulgaris) by natural fermentation. Eur Food Res Technol 214: 226-231.
  • Griga M, Kos turkova G, Kuchuk N, Ilieva-S toilova M (2001). Biotechnology. In: Carbohydrates in Grain Legume Seeds. Improving Nutritional Quality and Agronomic Characteris tics, Hedley CL (eds.), CAB Int. Wallingford, pp: 145-207.
  • Guillon F and Champ MMJ (2002). Carbohydrate fractions of legumes: uses in human nutrition and potential for health. B J Nutr 88: S293-S306.
  • Gulewicz P, Martinez-Villaluenga C, Kasprowicz- Potocka M and Frias J (2014). Non-Nutritive compounds in fabaceae family seeds and the improvementof their nutritional quality by traditional processing – a review. Pol J Food Nutr Sci 64: 75-89.
  • Gulewicz P, Szymaniec S, Bubak B, Frias J, Vidal- Valverde C, Trojanowska K and Gulewicz K (2002). Biological activity of α-galactoside preparations from Lupinus angus tifolius L. and Pisum sativum L. seeds. J Agric Food Chem 50: 384-389.
  • Haab CI and Keller F (2002). Purification and characterization of the raffinose oligosaccharide chain elongation enzyme, galactan:galactan galactosytransferase (GGT), from Ajuga reptans leaves. Physiol Plant 114: 361-371.
  • Hagely KB (2013). Improving Soybean seed composition through molecular breeding for raffinose family oligosaccharides, lectin and trypsin inhibitors. Dissertation, University of Missouri,Missouri. Hagely KB, Palmquis t D and Bilyeu KD (2013). Classification of dis tinct seed carbohydrate profiles in soybean.J Agric Food Chem 61: 1105- 1111.
  • Han IH and Baik B-K (2006). Oligosaccharide content and composition of legumes and their reduction by soaking, cooking, ultrasound, and high hydros tatic pressure. Cereal Chem 83: 428-433.
  • Hannah M A, Zuther E, Buchel K and Heyer AG (2006). Transport and metabolism of raffinose family oligosaccharides in transgenic potato. J Exp Bot 57: 3801-3811.
  • Hayakawa K, Mizutani J, Wada K, Masai T, Yoshiara I and Mitsuoka T (1990). Effects of soybean oligosaccharides on human fecal microflora. Microb Ecol Health D 3: 293-303.
  • Hitz WD, Carlson TJ, Kerr PS and Sebas tian SA (2002). Biochemical and molecular characterization of a mutation that confers a decreased raffinosaccharide and phytic acid phenotype on soybean seeds.Plant Physiol 128: 650-660.
  • Hoch G, Peterbauer T and Richter A (1999). Purification and characterization of s tachyose synthase from lentil (Lens culinaris) seeds: galactopinitol and s tachyose synthesis. Arch Biochem Biophy 366: 75-81.
  • Huynh B, Palmer L, Mather DE, Wallwork H, Graham RD, Welch RM and S tangoulis JCR (2008). Genotypic variation in wheat grain fructan content revealed by a simplified HPLC method. J Cereal Sci 48: 369-378.
  • Ibrahim SS, Habiba RA, Shatta AA and Embaby HE (2002). Effect of soaking, germination, cooking 4(1):70-88, 2018
  • and fermentation on antinutritional factors in cowpeas. Nahrung 46: 92-95.
  • Irish GC, Barbour G W, Classen HL, Tyler RT and Bedford MR (1995). Removal of the alphagalactosides of sucrose from soybean meal using either ethanol extraction or exogenous alphagalactosidase and broiler performance. Poult Sci 74: 1484-1494.
  • Jaureguy LM, Chen P and Scaboo AM (2011). Heritability and correlations among food-grade traits in soybean. Plant Breed 13: 647-652.
  • Jenkins DJA, Thorne MJ, Camelon K, Jenkins A, Venketeshwer-Rao A, Taylor RH, Thompson LU, Kalmusky J, Reichert R and Francis T (1982).
  • Effect of processing on diges tibility and the blood glucose response: A s tudy of lentils. Amer J Clin Nutr 36: 1093-1101.
  • Johnson CR, Thavarajah D, Combs Jr. GF and Thavarajah P (2013). Lentil (Lens culinaris L.): a prebiotic-rich whole food legume. Food Res Int 51: 107-113.
  • Jukanti AK, Gaur PM, Gowda CLL and Chibbar RN (2012). Nutritional quality and health benefits of chickpea (Cicer arietinum L.): a review. Br J Nutr 108: S11−S26.
  • Kadlec P, Dos tálová J, Houška M, S trohalm J and Bubník Z (2006). Evaluation of α-galactosides decrease during s torage of germinated pea seeds treated by high pressure.J Food Engin 77: 364- 367.
  • Kannan U, Sharma R, Khedikar YP, Gangola MP, Ganeshan S, Båga M and Chibbar RN (2016).
  • Differential expression of two galactinol synthase isoforms LcGolS1 and LcGolS2 in developing lentil (Lens culinaris Medik. cv CDC Redberry) seeds. Plant Physiol Biochem 108: 422-433.
  • Karner U, Peterbauer T, Raboy V, Jones DA, Hedley CL and Richter A (2004). Myo-inositol and sucrose concentrations affect the accumulation of raffinose family oligosaccharides in seeds. J Exp Bot 55: 1981-1987.
  • Keller F and Pharr DM (1996). Metabolism of carbohydrates in sinks and sources: galactosylsucrose oligosaccharides. In: Photoassimilate dis tribution in plants and crops, Zamski E, Schaffer AA (eds.), Marcel Dekker, New York, pp: 115-184.
  • Kerr PS and Sebas tian SA (1998). Soybean products with improved carbohydrate composition and soybean plants.International Patent Publication WO 93/07742, PCT/US92/08958.
  • Kerr PS, Pearls tein RW, Schweiger BJ, Becker-Manley MF and Pierce JW (1993). Nucleotide sequences of galactinol synthase from zucchini and soybean. International Patent Publication WO 93/02196, PCT/US92/06057.
  • Khalil AH and Mansour EH (1995). The effect of cooking, autoclaving and germination on the nutritional quality of faba beans. Food Chem 54: 177-182.
  • Kim WD, Kobayashi O, Kaneco S, Sakakibara Y, Park GG, Kusakabe I, Tanaka H and Kobayashi H (2002). Galactosidase from cultured rice (Oryza sativa L. Var. Nipponbare) cells. Phytochem 61: 621–630.
  • King MR, White BA, Blaschek HP, Chassy BM, Mackie RI and Cann IK (2002). Purification and characterization of a thermos table α-galactosidase from Thermoanaerobacterium polysaccharolyticum. J Agri Food Chem 50: 5676-5682.
  • Kumar V, Rani A, Goyal L, Dixit AK, Manjaya JG, Dev J and Swamy M (2010). Sucrose and raffinose family oligosaccharides (RFOs) in soybean seeds as influenced by genotype and growing location. J Agric Food Chem 58: 5081-5085.
  • Lahuta LB and Dzik T (2011). D-chiro-Inositol affects accumulation of raffinose family oligosaccharides in developing embryos of Pisum sativum. J Plant Physiol 168: 352-358.
  • Lahuta LB and Goszczyńska J (2009). Inhibition of raffinose family oligosaccharides and galactosyl pinitols breakdown delays germination of winter vetch (Vicia villosa Roth.) seeds. Acta Soc Botan Poloniae 78: 203-208.
  • Lahuta LB, Goszczyńska J and Horbowicz M (2010). Seed α-D-galactosides of selected Vicia species and enzymes involved in their biosynthesis.Acta Biol Cracoviensia Ser Bot 52: 27-35.
  • Lahuta LB, Horbowicz M, Gojło E, Goszczyńska J and Górecki RJ (2005). Exogenously applied D-pinitol and D-chiro-inositol modifies the accumulation of α-D-galactosides in developing tiny vetch (Vicia hirsuta [L.] S.F. Gray) seeds. Acta Soc Botan Poloniae 74: 287-296.
  • Leblanc JG, Garro MS, Silves troni A, Connes C, Piard JC, Sesma F and Savoy de Giori G (2004). Reduction of α-galactooligosaccharides in soyamilk by Lactobacillus fermentum CRL 722: In vitro and in vivo evaluation of fermented soyamilk. J Appl Microbiol 97: 876- 881.
  • Lehle L and Tanner W (1973). The function of myo-inositol in the biosynthesis of raffinose. Purification and characterization of galactinol:sucrose 6-galactosyltransferase from Vicia faba seeds. Eur J Biochem 38: 103-110.
  • Leske KL and Coon CN (1999). Nutrient content and protein and energy diges tibilities of ethanolextracted, low α-galactoside soybean meal as compared to intact soybean meal. Poult Sci 78: 1177-1183.
  • Leske KL, Zhang B and Coon CN (1995). The use of low α-galactoside protein products as a protein source in chicken diets. Anim Feed Sci Technol 54: 275-286.
  • Li S, Kim WD, Kaneko S, Prema PA, Nakajima M and Kobayashi H (2007). Expression of rice (Oryza sativa L. var. Nipponbare) alpha-galactosidase genes in Escherichia coli and characterization. Biosci Biotechnol Biochem 71: 520-526.
  • Liu Y, Sun Y, Li Y, Xu S, Tang J, Ding J and Xu Y (2011). Preparation and characterization of α-galactosidase-loaded chitosan nanoparticles for use in foods.Carbo Poly 83: 1162-1168.
  • Machaiah JP and Pednekar MD (2002). Carbohydrate composition of low dose radiation-processed legumes and reduction in flatulence factors. Food Chem 79: 293-301.
  • Machaiah JP, Pednekar MD and Thomas P (1999). Reduction in flatulence factors in mung beans (Vigna radiata) using low-dose γ-irradiation. J Sci Food Agric 79: 648-652.
  • Martín-Cabrejas MA, Aguilera Y, Benitez V, Molla E, Lopez-Andreu FJ and Es teban RM (2006). Effect of indus trial dehydration on soluble carbohydrates and dietary fibre fractions in legumes. J Agric Food Chem 54: 7652- 7657.
  • Martín-Cabrejas MA, Díaz MF, Aguilera Y, Benítez V, Mollá E and Es teban RM (2008). Influence of germination on the soluble carbohydrates and dietary fibre fractions in non-conventional legumes. Food Chem 107: 1045-1052.
  • Martín-Cabrejas MA, Sanfiz B, Vidal A, Molla E, Es teban RM and Lopez-Andreu FJ (2004). Effect of fermentation and autoclaving on dietary fibre fractions and antinutritional factors of beans (Phaseolus vulgaris L.) J Agric Food Chem 52: 261-266.
  • Martínez-Villaluenga C, Chicholoska J, Kliber A and Gulewicz K (2008a). Raffinose family oligosaccharides of lupin (Lupinus albus L. cv Multolupa) as a potential prebiotic. Proc Nutr Soc 67: E55.
  • Martínez-Villaluenga C, Frias J and Vidal-Valverde C (2008b). Alpha-galactosides: antinutritional factors or functional ingredients? Crit Rev Food Sci Nutr 48: 301-316.
  • McCleary X and Matheson NK (1974). α-d- Galactosidase activity and galactomannan and galactosyl sucrose oligosaccharide depletion in germinating legume seeds. Phytochem 13: 1747- 1757.
  • Mozzoni L, Shi A and Chen P (2013). Genetic Analysis of high sucrose, low raffinose, and low s tachyose content in V99-5089 soybean seeds. J Crop Improv 27: 606-616.
  • Mubarak AE (2005). Nutritional composition and antinutritional factors of mung bean seeds (Phaseolus aureus) as affected by some home traditional processes. Food Chem 89: 489-495.
  • Nishizawa A, Yabuta Y and Shigeoka S (2008). Galactinol and raffinose cons titute a novel function to protect plants from oxidative damage. Plant Physiol 147: 1251-1263.
  • Obendorf RL and Górecki RJ (2012). Soluble carbohydrates in legume seeds. Seed Sci Res 22: 219-242.
  • Onigbinde AO and Akinyele IO (1983). Oligosaccharide content of 20 varieties of cowpeas in Nigeria. J Food Sci 48: 1250-1254.
  • Onyenekwe PC, Njoku GC and Ameh DA (2000). Effect of cowpea (Vigna unguiculata) processing methods on flatus causing oligosaccharides. Nut Res 20: 349-358.
  • Oomah BD, Patras A, Rawson A, Singh N, Compos- Vega R (2011) Chemis try of pulses. In: Pulse Foods: Processing, quality, nutraceutical applications. Tiwari BK, Gowen A, McKenna B (eds.), Academic Press, London, UK, pp: 9- 56.
  • Oosumi C, Nozaki J and Kida T (1998). Raffinose synthetase gene, process for reducing raffinose, and transformed plant. International Patent Publication W098/49273, PCT/JP97/03879. 4(1):70-88, 2018
  • Parsons CM, Zhang Y and Araba M (2000). Nutritional evaluation of soybean meals varying in oligosaccharide content. Poult Sci 79:1127- 1131.
  • Peterbauer T and Richter A (1998). Galactosylononitol and s tachyose synthesis in seeds of adzuki bean. Purification and characterization of s tachyose synthase. Plant Physiol 117: 165-172.
  • Peterbauer T and Richter A (2001b). Biochemis try and physiology of raffinose family oligosaccharides and galactosyl cyclitols in seeds. Seed Sci Res 11: 185-197.
  • Peterbauer T, Karner U, Mucha J, Mach L, Jones DA, Hedley CL and Richter A (2003). Enzymatic control of the accumulation of verbascose in pea seeds. Plant Cell Environ 26: 1385-1391.
  • Peterbauer T, Mach L, Mucha J and Richter A (2002a). Functional expression of a cDNA encoding pea (Pisum sativum L.) raffinose synthase, partial purification of the enzyme from maturing seeds, and s teady-s tate kinetic analysis of raffinose synthesis. Planta 215: 839-846.
  • Peterbauer T, Mucha J, Mayer U, Popp M, Glössl J and Richter A (1999). S tachyose synthesis in seeds of adzuki bean (Vigna angularis): molecular cloning and functional expression of s tachyose synthase. Plant J 20: 509-518.
  • Peterbauer T, Lahuta LB, Blochl A, Mucha J, Jones DA, Hedley CL, Gorecki RJ and Richter A (2001a). Analysis of raffinose family oligosaccharide pathway in pea seeds with contras ting carbohydrate composition. Plant Physiol 127: 1764-1772.
  • Peterbauer T, Mucha J, Mach L and Richter A (2002b). Chain elongation of raffinose in pea seeds: isolation, characterization, and molecular cloning of a multifunctional enzyme catalyzing the synthesis of s tachyose and verbascose. J Biol Chem 277: 194-200.
  • Peters S, Egert A, S tieger B and Keller F (2010). Functional identification of Arabidopsis ATSIP2 (At3g57520) as an alkaline α-galactosidase with a subs trate specificity for raffinose and an apparent sink-specific expression pattern. Plant Cell Physiol 51: 1815-1819.
  • Polowick PL, Baliski DS, Bock C, Heather R and Fawzy G (2009). Over-expression of α-galactosidase in pea seeds to reduce raffinose oligosaccharide content. Botany/Botanoque 87: 526-532.
  • Qiu D, Vuong T, Valliyodan B, Shi H, Guo B, Shannon JG and Nguyen HT (2015). Identification and characterization of a s tachyose synthase gene controlling reduced s tachyose content in soybean. Theor Appl Genet 128: 2167-2176.
  • Quemener B and Brillouet J (1983). Ciceritol, a pinitol digalactoside form seeds of chickpea, lentil and white lupin. Phytochem 22: 1745-1751.
  • Rao VS and Vakil UK (1983). Effects of gammairradiation on flatulence causing oligosaccharides in green gram (Phaseolus aureus). J Food Sci 48: 1791-1795.
  • Reddy NR and Salunkhe DK (1980). Changes in oligosaccharides during germination and cooking of black gram and fermentation of black gram/ rice blend.Cereal Chem 57: 356-360.
  • Reddy NR, Pierson MD, Sathe SK and Salunkhe DK (1984). Chemical, nutritional and physiological aspects of dry bean carbohydrates: a review. Food Chem 13: 25-68.
  • Rehman Z and Shah WH (2005). Thermal heat processing effects on antinutrients, protein and s tarch diges tibility of food legumes. Food Chem 91: 327-331.
  • Richter A, Hoch G, Puschenreiter M, Mayer U and Peterbauer T (2000). The role of s tachyose synthase in the oligosaccharide metabolism of legume seeds. In: Seed biology: Advances and applications, Black M, Bradford KJ and Vásquez- Ramos J (eds.), CAB Int, Wallingford, pp: 75-84.
  • Roberfroid MB (1999). Concepts in Functional Foods: The Case of Inulin and Oligofructose. J Nutr 129: 1398S-1401S.
  • Roberfroid MB, Van Loo JAE and Gibson GR (1998). The bifidogenic nature of chicory inulin and its hydrolysis products. J Nutr 128: 11-19.
  • Saini HS and Knights EJ (1984). Chemical cons titution of s tarch and oligosaccharide components of ‘desi’ and ‘kabuli’ chickpea (Cicer arietinum) seed types. J Agric Food Chem 32: 940-944.
  • Sánchez-Mata MC, Cámara MM and Díez-Marqués C (1999). Effect of domes tic processes and water hardness on soluble sugars contents of chickpeas (Cicer arietinum L.). Food Chem 65: 331-338.
  • Sebas tian SA, Kerr PS, Pearls tein RW and Hitz WD (2000). Soybean germplasm with novel genes for improved diges tibility. In: Soy in animal nutrition, Drackley JK (eds.), Federation of Animal Science Societies, Savoy, pp: 56-74.
  • Sengupta S, Mukherjee S, Basak P and Majumder AL (2015) Significance of galactinol and raffinose family oligosaccharide synthesis in plants. Front Plant Sci. 6: 656.
  • Shewry PR and Halford NG (2002). Cereal seed s torage proteins: s tructures, properties and role in grain utilization. J Exp Bot 53: 947-958.
  • Shimelis EA and Rakshit SK (2007). Effect of processing on antinutrients and in vitro protein diges tibility of kidney bean (Phaseolus vulgaris L.) varieties grown in Eas t Africa. Food Chem 103: 161-172.
  • Smiricky MR, Grieshop CM, Albin DM, Wubben, JE, Gabert VM and Fahey GCJ (2002). The influence of soy oligosaccharides on apparent and true ileal amino acid diges tibilities and fecal consis tency in growing pigs. J Animal Sci 80: 2433-2441.
  • Sosulski FW, Elkowicz L and Reichert RD (1982). Oligosaccharides in eleven legumes and their air-classified protein and s tarch fractions. J Food Sci 47: 498-502.
  • Sprenger N and Keller F (2000). Allocation of raffinose family oligosaccharides to transport and s torage pools in Ajuga reptans: the roles of two dis tinct galactinol synthases. Plant J 21: 249-258.
  • Tahir M, Båga M, Vandenberg A and Chibbar RN (2012). An assessment of raffinose family oligosaccharides and sucrose concentration in genus Lens. Crop Sci 52: 1713- 1720.
  • Tahir M, Vandenberg A and Chibbar RN (2011). Influence of environment on seed soluble carbohydrates in selected lentil cultivars. J Food Compos Anal 24: 596-602.
  • Taji T, Ohsumi C, Iuchi S, Seki M, Kasuga M, Kobayashi M, Yamaguchi-Shinozaki K and Shinozaki K (2002). Important roles of droughtand cold-inducible genes for galactinol synthase in s tress tolerance in Arabidopsis thaliana. Plant J 29: 417-426.
  • Tanner W and Kandler O (1968). Myo-Inositol, a cofactor in the biosynthesis of s tachyose. Eur J Biochem 4: 233-239.
  • Tapernoux-Lüthi EM, Schneider T and Keller F (2007). The C-terminal sequence from common bugle leaf galactan:galactan galactosyltransferase is a non-sequence-specific vacuolar sorting determinant. FEBS Letters 581: 1811-1818.
  • Torres A, Frias J, Granito M and Vidal-Valverde C (2006). Fermented pigeon pea (C. cajan) ingredients in pas ta products. J Agric Food Chem 54: 6685-6691.
  • Ueda T, Coseo MP, Thomas JH and Obendorf RL (2005). A multifunctional galactinol synthase catalyzes the synthesis of fagopyritol A1 and fagopyritol B1 in buckwheat seed. Plant Sci 168: 681-690.
  • Upadhyay JK and Garcia VV (1988). Effect of soaking and cooking on reduction of oligosaccharides of cowpea (Vigna unguiculata (L) Walp). Philip J Food Sci & Tech 12: 21-28.
  • Urbano G, Lopez-Jurado M, Hernandez J, Fernandez M, Moreu MC, Frias J, Diaz-Pollan C, Prodanov M and Vidal-Valverde C (1995). Nutritional assessment of raw, heated, and germinated lentils. J Agric Food Chem 43: 1871-1877.
  • Van Barneveld RJ (1999). Unders tanding the nutritional chemis try of lupin (Lupinus spp.) seed to improve lives tock production efficiency. Nutr Res Rev 12: 203-230.
  • Veldman A, Veen WAG, Barug D and Van Paridon PA (1993).Effect of α-galactosides and α-galactosidase in feed on ileal piglet diges tive physiology. J Animal Physiol Animal Nutr 69: 57-65.
  • Viana PA, Tavares de Rezende S, Falkoski DL, Leite T, José IC, Moreira MA and Guimarães VM (2007). Hydrolysis of oligosaccharides in soybean products by Debaryomyces hansenii UFV-1 α-galactosidases. Food Chem 103: 331-337.
  • Viana SF, Guimarães VM, José IC, Oliveira MGA, Cos ta NMB, Barros EG, Alves MM and Rezende S (2005). Hydrolysis of oligosaccharides in soybean flour by soybean a-galactosidase. Food Chem 93: 665-670.
  • Vidal-Valverde C, Frias J, Hernández A, Martín- Alvarez PJ, Sierra I, Rodríguez C, Blazquez I and Vicente G (2003). Assessment of nutritional compounds and antinutritional factors in pea (Pisum sativum) seeds. J Sci Food Agric 83: 298-306.
  • Vidal-Valverde C, Frias J, Sotomayor C, Diaz-Pollan C, Fernandez M and Urbano G (1998). Nutrients and antinutritional factors in faba beans as affected by processing. Z Lebensm Unters Forsch 207: 140-145.
  • Vijayakumari K, Pugalenthi M and Vadivel V (2007). Effect of soaking and hydrothermal processing methods on the levels of antinutrients and in vitro protein diges tibility of Bauhinia purpurea L. seeds. Food Chem 103: 968- 975.
  • Wada K, Watabe J, Mizutani J, Suzuki H, Kiriku N, Hayakawa K and Yamaguchi C (1991). Effects of soybean oligosaccharides intake on fecal microflora, enzyme activity, ammonia and frequency of evacuation in elderly persons. Bifidus 4: 135-140.
  • Wang N, Hatcher DW, Gawalko EJ (2008). Effect of variety and processing on nutrients and certain anti-nutrients in field peas (Pisum sativum).Food Chem 111: 132-138.
  • Wang N, Hatcher DW, Toews R and Gawalko EJ (2009). Influence of cooking and dehulling on nutritional composition of several varieties of lentils (Lens culinaris). Food Sci Tech 42: 842-848.
  • Wang N, Hatcher DW, Tyler RT, Toews R and Gawalko EJ (2010). Effect of cooking on the composition of beans (Phaseolus vulgaris L.) and chickpeas (Cicer arietinum L.).Food Res Int 43: 589-594.
  • Wang N, Lewis MJ, Brennan JG and Wes tby A (1997). Effect of processing methods on nutrients and anti-nutritional factors in cowpea. Food Chem 58: 59-68.
  • Wang TL, Domoney C, Hedley CL, Casey R and Grusak MA (2003). Can we improve the nutritional quality of legume seeds? Plant Physiol 131: 886–891.
  • Wiggins HS (1984). Nutritional value of sugars and related compounds undiges ted in the small intes tine. Proc Nutr Soc 43: 69-75.
  • Yamaguishi CT, Sanada CT, Gouvêa PM, Pandey A, Woiciechowski AL, Parada JL and Soccol CR (2009). Biotechnological process for producing black bean slurry without s tachyose. Food Res Intern 42: 425-429.
  • Youssef MM, Hamza MA, Abdel-Aal MH, Shekib LA and El-Banna AA (1986). Amino acid composition and in vitro diges tibility of some Egyptian foods made from faba bean (Vicia faba L.). Food Chem 22: 225-233.
  • Zhao T, Martin D, Meeley RB and Downie B (2004). Expression of the maize galactinol synthase gene family: (II) Kernel abscission, environmental s tress and myo-inositol influences accumulation of transcript in developing seeds and callus cells. Physiol Plant 121: 647-655.
  • Zuo Y, Fahey GC, Merchen NR and Bajjalieh NL (1996). Diges tion responses to low oligosaccharide soybean meal by ileally-cannulated dogs. J Anim Sci 74: 2441-2449.
Year 2018, Volume: 4 Issue: 1, 70 - 88, 30.01.2018

Abstract

References

  • Aguilera Y, Martín-Cabrejas MA, Benítez V, Mollá E, López-Andréu FJ and Es teban RM (2009). Changes in carbohydrate fraction during dehydration process of common legumes. J Food Compos Anal 22: 678-683.
  • Alajaji SA and El-Adawy TA (2006). Nutritional composition of chickpea (Cicer arietinum L.) as affected by microwave cooking and other traditional cooking methods. J Food Compos Anal 19: 806-812.
  • Al-Kaisey MT, Alwan AKH, Mohammad MH and Saeed AH (2003). Effect of gamma irradiation on antinutritional factors in broad beans. Radiat Phys Chem 67: 493-496.
  • Allan SM and Hitz WD (2000). Plant raffinose synthase homologs. International Patent Publication WO 00/24915, PCT/US99/24923. Andersen KE, Bjergegaard C, Moller P, Sorensen JC and Sorensen H (2005). Compositional variations for α-galactosides in different species of leguminosae, brassicaceae, and barley: a chemotaxonomic s tudy based on chemometrics and high-performance capillary electrophoresis. J Agric Food Chem 53: 5809-5817.
  • Andrews M and Andrews ME (2017). Specificity in Legume-Rhizobia symbioses. Int J Mol Sci 18: 705.
  • Anisha GS and Prema P (2008). Reduction of nondiges tible oligosaccharides in horse gram and green gram flours using crude α-galactosidase from S treptomyces griseoloalbus. Food Chem 106: 1175-1179.
  • Aranda P, Dos talova J, Frias J, Lopez-Jurado M, Kozłowska H, Pokorny J, Urbano G, Vidal- Valverde C and Zduńczyk Z (2001). Nutrition. In: Carbohydrates in grain legume seeds. Improving nutritional quality and agronomic characteris tics, Hedley CL (eds.), CAB Int. Wallingford, pp: 61-87.
  • Avigad G and Dey PM (1997). Carbohydrate metabolism: S torage carbohydrates. In: Plant biochemis try, Dey PM and Harbourne J (eds.), Academic Press, San Diego, pp: 143-204.
  • Bachmann M and Keller F (1995). Metabolism of the raffinose family oligosaccharides in leaves of Ajuga reptans L. Inter- and intracellular compartmentation. Plant Physiol 109: 991-998.
  • Bachmann M, Matile P and Keller F (1994). Metabolism of the raffinose family oligosaccharides in leaves of Ajuga reptans L. Cold acclimation, translocation, and sink to source transition: Discovery of a chain elongation enzyme. Plant Physiol 105: 1335-1345.
  • Bilyeu KD and Wiebold WJ (2016). Environmental s tability of seed carbohydrate profiles in soybeans containing different alleles of the raffinose synthase 2 (RS2) gene. J Agri Food Chem 64: 1071–1078.
  • Blӧchl A, Peterbauer T and Richter A (2007). Inhibition of raffinose oligosaccharide breakdown delays germination of pea seeds. J Plant Physiol 164: 1093-1096.
  • Bock C, Ray H and Georges F (2009). Down-regulation of galactinol synthesis in oilseed Brassica napus leads to significant reduction of antinutritional oligosaccharides.Botany 87: 597-603.
  • Brasil APR, Tavares de Rezende S, Gouveia Pelúzio M and Guimarães VM (2010). Removal of oligosaccharides in soybean flour and nutritional effects in rats. Food Chem 118: 251-255.
  • Cao Y, Yuan T, Shi P, Luo H, Li N, Meng K, Bai Y, Yang P, Zhou Z, Zhang Z and Yao B (2010). Properties of a novel α-galactosidase from S treptomyces sp. S27 and its potential for soybean processing. Enzy Micro Techn 47: 305-312.
  • Cas tillo EM, De Lumen BO, Reyes PS and De Lumen HZ (1990). Raffinose synthase and galactinol synthase in developing seeds and leaves of legumes. J Agric Food Chem 38: 351-355.
  • Chibbar RN, Ambigaipalan P and Hoover R (2010). Molecular diversity in pulse seed s tarch and complex carbohydrates and its role in human nutrition and health. Cereal Chem 87: 342- 352.
  • Chilomer K, Zaleska K, Ciesiołka D, Gulewicz P, Frankiewicz A and Gulewicz K (2010). Changes in the alkaloid, α-galactoside and protein fraction content during germination of different lupin spcies. Acta Soc Bot Pol 79: 11-20.
  • Cicek MS, Chen P, Saghai Maroof MA and Buss GR (2006). Interrelationships among agronomic and seed quality traits in an interspecific soybean recombinant inbred population. Crop Sci 46: 1253-1259.
  • Clarke EJ and Wiseman J (2000). Developments in plant breeding for improved nutritional quality of soya beans II. Anti-nutritional factors.J Agri Sci Camb 134: 125-136. 4(1):70-88, 2018
  • Coon CN, Leske KL, Akavanichan O and Cheng TK (1990). Effect of oligosaccharides-free soybean meal on true metabolizable energy and fibre diges tion in adult roos ters. Poult Sci 69: 787-793.
  • Das A and Parida SK (2014). Advances in biotechnological applications in three important food legumes.Plant Biotechnol Rep 8:83–99.
  • De Faria SM, Lewis GP, Sprent JI and Sutherland JM (1989). Occurrence of nodulation in the leguminosae. New Phytol 111: 607-619.
  • Devindra, S, Rao JS, Krishnaswamya P and Bhaskarb V (2011). Reduction of α-galactoside content in red gram (Cajanus cajan L.) upon germination followed by heat treatment. J Sci Food Agric 91: 1829-1835.
  • Dey PM and Campillo ED (1984). Biochemis try of the multiple forms of glycosidases in plants. Adv Enzymol Relat Areas Mol Biol 56: 141-249.
  • Dharmasena MN and Mathew CD (2002). Reducing flatulence factors in soymilk using coconut kernel α-galactosidase immobilised hydrophobic gels. J Natn Sci Foundation 30(3&4): 161-169.
  • Dierking EC and Bilyeu KD (2008). Association of a soybean raffinose synthase gene with low raffinose and s tachyose seed phenotype. Plant Genome 1: 135–145.
  • Dierking EC and Bilyeu KD (2009). New sources of soybean seed meal and oil composition traits identified through TILLING. BMC Plant Biol 9:89.
  • Dilis V and Trichopoulou A (2009). Nutritional and health properties of pulses. Mediterr. J Nutr Metab 1: 149-157.
  • Dos Santos TB, Budzinski IG, Marur CJ, Petkowicz CL, Pereira LF and Vieira LG (2011). Expression of three galactinol synthase isoforms in Coffea arabica L. and accumulation of raffinose and s tachyose in response to abiotic s tresses. Plant Physiol Biochem 49: 441-448.
  • Downie B, Gurusinghe S, Dahal P, Thacker RR, Snyder JC, Nonogaki H, Yim K, Fukanaga K, Alvarado V and Bradford KJ (2003). Expression of a galactinol synthase gene in tomato seeds is upregulated before maturation desiccation and again after imbibition whenever radicle protrusion is prevented. Plant Physiol 131: 1347-59.
  • Egbert A, Keller F and Peters S (2013). Abiotic s tressinduced accumulation of raffinose in Arabidopsis leaves is mediated by a single raffinose synthase (RS5, At5g40390). BMC Plant Biol 13: 218.
  • El-Adawy TA (2002). Nutritional composition and antinutritional factors of chickpeas (Cicer arietinum L.) undergoing different cooking methods and germination. Plant Foods Hum Nutr 57: 83-97.
  • ElSayed AI, Rafudeen MS and Golldack D (2014). Physiological aspects of raffinose family oligosaccharides in plants: protection agains t abiotic s tress. Plant Biol 16: 1–8.
  • Falkoski DL, Guimarães VM, Callegari CM, Reis AP, Barros EG and Rezende S (2006). Processing of soybean products by semipurified plant and microbial α-galactosidases.J Agric Food Chem 54: 10184-10190.
  • FAOSTAT (2016). http://www.fao.org/faos tat/en/#data/ QC (accessed on April 26, 2017).
  • Frias J, Bakhash A, Jones DA, Arthur AE, Vidal- Valverde C, Rhodes MJC and Hedley CL (1999). Genetic analysis of the raffinose oligosaccharide pathway in lentil seeds. J Exp Bot 50: 469-476.
  • Frias J, Giacomino S, Peñas E, Pellegrino N, Ferreyra N, Apro V, Olivera-Carrión M and Vidal-Valverde C (2011). Assessment of the nutritional quality of raw and extruded Pisum sativum L. var. Laguna seeds. LWT – Food Sci. Technol 44: 1303-1308.
  • Frias J, Vidal-Valverde C, Sotomayor C, Diaz-Pollan C and Urbano G (2000). Influence of processing on available carbohydrate content and antinutritional factors of chickpeas. Eur Food Res Technol 210: 340-345.
  • Gangola MP, Behl RK, Chibbar RN (2014a) S trategies to improve seed quality of pulse crops to ensure global food and nutrition security. In: Cutting edge science and technologies for food, environment and health, Behl RK, Asif AR and Merbach W (eds.), Agrobios (International), Jodhpur, India, pp: 7-21.
  • Gangola MP, Jaiswal S, Kannan U, Gaur PM, Båga M and Chibbar RN (2016). Galactinol synthase enzyme activity influences raffinose family oligosaccharides (RFO) accumulation in developing chickpea (Cicer arietinum L.) seeds. Phytochem 125: 88-98.
  • Gangola MP, Jaiswal S, Khedikar YP and Chibbar RN (2014b) A reliable and rapid method for soluble sugars and RFO analysis in chickpea using HPAEC–PAD and its comparison with HPLC–RI. Food Chem 154: 127–133.
  • Gangola MP, Khedikar YP, Gaur PM, Båga M and Chibbar RN (2012). Variation in important seed cons tituents among various chickpea genotypes. Qual Assur Saf Crop 4: 136–158.
  • Gangola MP, Khedikar YP, Gaur PM, Båga M and Chibbar RN (2013). Genotype and growing environment interaction shows a positive correlation between subs trates of raffinose family oligosaccharides (RFO) biosynthesis and their accumulation in chickpea (Cicer arietinum L.) seeds. J Agric Food Chem 61: 4943–4952.
  • Gil L, Ben-Ari J, Turgeon R and Wolf S (2012). Effect of CMV infection and high temperatures on the enzymes involved in raffinose family oligosaccharide biosynthesis in melon plants. J Plant Physiol 169: 965-970.
  • Girigowda K, Prashanth SJ and Mulimani VH (2005). Oligosaccharins of black gram (Vigna mungo L.) as affected by processing methods. Plant Foods Hum Nutr 60: 173-80.
  • Glencross BD, Boujard T and Kaushik SJ (2003). Influence of oligosaccharides on the diges tibility of lupin meals when fed to rainbow trout Oncorhynchus mykiss. Aquaculture 219: 703-713. Gote M, Umalkar H, Khan I and Khire J (2004).
  • Thermos table α-galactosidase from Bacillus s tearothermophilus (NCIM 5146) and its application in the removal of flatulence causing factors from soymilk. Process Biochem 39: 1723- 1729.
  • Granito M, Frias J, Doblado R, Guerra M, Champ M and Vidal–Valverde C (2002). Nutritional improvement of beans (Phaseolus vulgaris) by natural fermentation. Eur Food Res Technol 214: 226-231.
  • Griga M, Kos turkova G, Kuchuk N, Ilieva-S toilova M (2001). Biotechnology. In: Carbohydrates in Grain Legume Seeds. Improving Nutritional Quality and Agronomic Characteris tics, Hedley CL (eds.), CAB Int. Wallingford, pp: 145-207.
  • Guillon F and Champ MMJ (2002). Carbohydrate fractions of legumes: uses in human nutrition and potential for health. B J Nutr 88: S293-S306.
  • Gulewicz P, Martinez-Villaluenga C, Kasprowicz- Potocka M and Frias J (2014). Non-Nutritive compounds in fabaceae family seeds and the improvementof their nutritional quality by traditional processing – a review. Pol J Food Nutr Sci 64: 75-89.
  • Gulewicz P, Szymaniec S, Bubak B, Frias J, Vidal- Valverde C, Trojanowska K and Gulewicz K (2002). Biological activity of α-galactoside preparations from Lupinus angus tifolius L. and Pisum sativum L. seeds. J Agric Food Chem 50: 384-389.
  • Haab CI and Keller F (2002). Purification and characterization of the raffinose oligosaccharide chain elongation enzyme, galactan:galactan galactosytransferase (GGT), from Ajuga reptans leaves. Physiol Plant 114: 361-371.
  • Hagely KB (2013). Improving Soybean seed composition through molecular breeding for raffinose family oligosaccharides, lectin and trypsin inhibitors. Dissertation, University of Missouri,Missouri. Hagely KB, Palmquis t D and Bilyeu KD (2013). Classification of dis tinct seed carbohydrate profiles in soybean.J Agric Food Chem 61: 1105- 1111.
  • Han IH and Baik B-K (2006). Oligosaccharide content and composition of legumes and their reduction by soaking, cooking, ultrasound, and high hydros tatic pressure. Cereal Chem 83: 428-433.
  • Hannah M A, Zuther E, Buchel K and Heyer AG (2006). Transport and metabolism of raffinose family oligosaccharides in transgenic potato. J Exp Bot 57: 3801-3811.
  • Hayakawa K, Mizutani J, Wada K, Masai T, Yoshiara I and Mitsuoka T (1990). Effects of soybean oligosaccharides on human fecal microflora. Microb Ecol Health D 3: 293-303.
  • Hitz WD, Carlson TJ, Kerr PS and Sebas tian SA (2002). Biochemical and molecular characterization of a mutation that confers a decreased raffinosaccharide and phytic acid phenotype on soybean seeds.Plant Physiol 128: 650-660.
  • Hoch G, Peterbauer T and Richter A (1999). Purification and characterization of s tachyose synthase from lentil (Lens culinaris) seeds: galactopinitol and s tachyose synthesis. Arch Biochem Biophy 366: 75-81.
  • Huynh B, Palmer L, Mather DE, Wallwork H, Graham RD, Welch RM and S tangoulis JCR (2008). Genotypic variation in wheat grain fructan content revealed by a simplified HPLC method. J Cereal Sci 48: 369-378.
  • Ibrahim SS, Habiba RA, Shatta AA and Embaby HE (2002). Effect of soaking, germination, cooking 4(1):70-88, 2018
  • and fermentation on antinutritional factors in cowpeas. Nahrung 46: 92-95.
  • Irish GC, Barbour G W, Classen HL, Tyler RT and Bedford MR (1995). Removal of the alphagalactosides of sucrose from soybean meal using either ethanol extraction or exogenous alphagalactosidase and broiler performance. Poult Sci 74: 1484-1494.
  • Jaureguy LM, Chen P and Scaboo AM (2011). Heritability and correlations among food-grade traits in soybean. Plant Breed 13: 647-652.
  • Jenkins DJA, Thorne MJ, Camelon K, Jenkins A, Venketeshwer-Rao A, Taylor RH, Thompson LU, Kalmusky J, Reichert R and Francis T (1982).
  • Effect of processing on diges tibility and the blood glucose response: A s tudy of lentils. Amer J Clin Nutr 36: 1093-1101.
  • Johnson CR, Thavarajah D, Combs Jr. GF and Thavarajah P (2013). Lentil (Lens culinaris L.): a prebiotic-rich whole food legume. Food Res Int 51: 107-113.
  • Jukanti AK, Gaur PM, Gowda CLL and Chibbar RN (2012). Nutritional quality and health benefits of chickpea (Cicer arietinum L.): a review. Br J Nutr 108: S11−S26.
  • Kadlec P, Dos tálová J, Houška M, S trohalm J and Bubník Z (2006). Evaluation of α-galactosides decrease during s torage of germinated pea seeds treated by high pressure.J Food Engin 77: 364- 367.
  • Kannan U, Sharma R, Khedikar YP, Gangola MP, Ganeshan S, Båga M and Chibbar RN (2016).
  • Differential expression of two galactinol synthase isoforms LcGolS1 and LcGolS2 in developing lentil (Lens culinaris Medik. cv CDC Redberry) seeds. Plant Physiol Biochem 108: 422-433.
  • Karner U, Peterbauer T, Raboy V, Jones DA, Hedley CL and Richter A (2004). Myo-inositol and sucrose concentrations affect the accumulation of raffinose family oligosaccharides in seeds. J Exp Bot 55: 1981-1987.
  • Keller F and Pharr DM (1996). Metabolism of carbohydrates in sinks and sources: galactosylsucrose oligosaccharides. In: Photoassimilate dis tribution in plants and crops, Zamski E, Schaffer AA (eds.), Marcel Dekker, New York, pp: 115-184.
  • Kerr PS and Sebas tian SA (1998). Soybean products with improved carbohydrate composition and soybean plants.International Patent Publication WO 93/07742, PCT/US92/08958.
  • Kerr PS, Pearls tein RW, Schweiger BJ, Becker-Manley MF and Pierce JW (1993). Nucleotide sequences of galactinol synthase from zucchini and soybean. International Patent Publication WO 93/02196, PCT/US92/06057.
  • Khalil AH and Mansour EH (1995). The effect of cooking, autoclaving and germination on the nutritional quality of faba beans. Food Chem 54: 177-182.
  • Kim WD, Kobayashi O, Kaneco S, Sakakibara Y, Park GG, Kusakabe I, Tanaka H and Kobayashi H (2002). Galactosidase from cultured rice (Oryza sativa L. Var. Nipponbare) cells. Phytochem 61: 621–630.
  • King MR, White BA, Blaschek HP, Chassy BM, Mackie RI and Cann IK (2002). Purification and characterization of a thermos table α-galactosidase from Thermoanaerobacterium polysaccharolyticum. J Agri Food Chem 50: 5676-5682.
  • Kumar V, Rani A, Goyal L, Dixit AK, Manjaya JG, Dev J and Swamy M (2010). Sucrose and raffinose family oligosaccharides (RFOs) in soybean seeds as influenced by genotype and growing location. J Agric Food Chem 58: 5081-5085.
  • Lahuta LB and Dzik T (2011). D-chiro-Inositol affects accumulation of raffinose family oligosaccharides in developing embryos of Pisum sativum. J Plant Physiol 168: 352-358.
  • Lahuta LB and Goszczyńska J (2009). Inhibition of raffinose family oligosaccharides and galactosyl pinitols breakdown delays germination of winter vetch (Vicia villosa Roth.) seeds. Acta Soc Botan Poloniae 78: 203-208.
  • Lahuta LB, Goszczyńska J and Horbowicz M (2010). Seed α-D-galactosides of selected Vicia species and enzymes involved in their biosynthesis.Acta Biol Cracoviensia Ser Bot 52: 27-35.
  • Lahuta LB, Horbowicz M, Gojło E, Goszczyńska J and Górecki RJ (2005). Exogenously applied D-pinitol and D-chiro-inositol modifies the accumulation of α-D-galactosides in developing tiny vetch (Vicia hirsuta [L.] S.F. Gray) seeds. Acta Soc Botan Poloniae 74: 287-296.
  • Leblanc JG, Garro MS, Silves troni A, Connes C, Piard JC, Sesma F and Savoy de Giori G (2004). Reduction of α-galactooligosaccharides in soyamilk by Lactobacillus fermentum CRL 722: In vitro and in vivo evaluation of fermented soyamilk. J Appl Microbiol 97: 876- 881.
  • Lehle L and Tanner W (1973). The function of myo-inositol in the biosynthesis of raffinose. Purification and characterization of galactinol:sucrose 6-galactosyltransferase from Vicia faba seeds. Eur J Biochem 38: 103-110.
  • Leske KL and Coon CN (1999). Nutrient content and protein and energy diges tibilities of ethanolextracted, low α-galactoside soybean meal as compared to intact soybean meal. Poult Sci 78: 1177-1183.
  • Leske KL, Zhang B and Coon CN (1995). The use of low α-galactoside protein products as a protein source in chicken diets. Anim Feed Sci Technol 54: 275-286.
  • Li S, Kim WD, Kaneko S, Prema PA, Nakajima M and Kobayashi H (2007). Expression of rice (Oryza sativa L. var. Nipponbare) alpha-galactosidase genes in Escherichia coli and characterization. Biosci Biotechnol Biochem 71: 520-526.
  • Liu Y, Sun Y, Li Y, Xu S, Tang J, Ding J and Xu Y (2011). Preparation and characterization of α-galactosidase-loaded chitosan nanoparticles for use in foods.Carbo Poly 83: 1162-1168.
  • Machaiah JP and Pednekar MD (2002). Carbohydrate composition of low dose radiation-processed legumes and reduction in flatulence factors. Food Chem 79: 293-301.
  • Machaiah JP, Pednekar MD and Thomas P (1999). Reduction in flatulence factors in mung beans (Vigna radiata) using low-dose γ-irradiation. J Sci Food Agric 79: 648-652.
  • Martín-Cabrejas MA, Aguilera Y, Benitez V, Molla E, Lopez-Andreu FJ and Es teban RM (2006). Effect of indus trial dehydration on soluble carbohydrates and dietary fibre fractions in legumes. J Agric Food Chem 54: 7652- 7657.
  • Martín-Cabrejas MA, Díaz MF, Aguilera Y, Benítez V, Mollá E and Es teban RM (2008). Influence of germination on the soluble carbohydrates and dietary fibre fractions in non-conventional legumes. Food Chem 107: 1045-1052.
  • Martín-Cabrejas MA, Sanfiz B, Vidal A, Molla E, Es teban RM and Lopez-Andreu FJ (2004). Effect of fermentation and autoclaving on dietary fibre fractions and antinutritional factors of beans (Phaseolus vulgaris L.) J Agric Food Chem 52: 261-266.
  • Martínez-Villaluenga C, Chicholoska J, Kliber A and Gulewicz K (2008a). Raffinose family oligosaccharides of lupin (Lupinus albus L. cv Multolupa) as a potential prebiotic. Proc Nutr Soc 67: E55.
  • Martínez-Villaluenga C, Frias J and Vidal-Valverde C (2008b). Alpha-galactosides: antinutritional factors or functional ingredients? Crit Rev Food Sci Nutr 48: 301-316.
  • McCleary X and Matheson NK (1974). α-d- Galactosidase activity and galactomannan and galactosyl sucrose oligosaccharide depletion in germinating legume seeds. Phytochem 13: 1747- 1757.
  • Mozzoni L, Shi A and Chen P (2013). Genetic Analysis of high sucrose, low raffinose, and low s tachyose content in V99-5089 soybean seeds. J Crop Improv 27: 606-616.
  • Mubarak AE (2005). Nutritional composition and antinutritional factors of mung bean seeds (Phaseolus aureus) as affected by some home traditional processes. Food Chem 89: 489-495.
  • Nishizawa A, Yabuta Y and Shigeoka S (2008). Galactinol and raffinose cons titute a novel function to protect plants from oxidative damage. Plant Physiol 147: 1251-1263.
  • Obendorf RL and Górecki RJ (2012). Soluble carbohydrates in legume seeds. Seed Sci Res 22: 219-242.
  • Onigbinde AO and Akinyele IO (1983). Oligosaccharide content of 20 varieties of cowpeas in Nigeria. J Food Sci 48: 1250-1254.
  • Onyenekwe PC, Njoku GC and Ameh DA (2000). Effect of cowpea (Vigna unguiculata) processing methods on flatus causing oligosaccharides. Nut Res 20: 349-358.
  • Oomah BD, Patras A, Rawson A, Singh N, Compos- Vega R (2011) Chemis try of pulses. In: Pulse Foods: Processing, quality, nutraceutical applications. Tiwari BK, Gowen A, McKenna B (eds.), Academic Press, London, UK, pp: 9- 56.
  • Oosumi C, Nozaki J and Kida T (1998). Raffinose synthetase gene, process for reducing raffinose, and transformed plant. International Patent Publication W098/49273, PCT/JP97/03879. 4(1):70-88, 2018
  • Parsons CM, Zhang Y and Araba M (2000). Nutritional evaluation of soybean meals varying in oligosaccharide content. Poult Sci 79:1127- 1131.
  • Peterbauer T and Richter A (1998). Galactosylononitol and s tachyose synthesis in seeds of adzuki bean. Purification and characterization of s tachyose synthase. Plant Physiol 117: 165-172.
  • Peterbauer T and Richter A (2001b). Biochemis try and physiology of raffinose family oligosaccharides and galactosyl cyclitols in seeds. Seed Sci Res 11: 185-197.
  • Peterbauer T, Karner U, Mucha J, Mach L, Jones DA, Hedley CL and Richter A (2003). Enzymatic control of the accumulation of verbascose in pea seeds. Plant Cell Environ 26: 1385-1391.
  • Peterbauer T, Mach L, Mucha J and Richter A (2002a). Functional expression of a cDNA encoding pea (Pisum sativum L.) raffinose synthase, partial purification of the enzyme from maturing seeds, and s teady-s tate kinetic analysis of raffinose synthesis. Planta 215: 839-846.
  • Peterbauer T, Mucha J, Mayer U, Popp M, Glössl J and Richter A (1999). S tachyose synthesis in seeds of adzuki bean (Vigna angularis): molecular cloning and functional expression of s tachyose synthase. Plant J 20: 509-518.
  • Peterbauer T, Lahuta LB, Blochl A, Mucha J, Jones DA, Hedley CL, Gorecki RJ and Richter A (2001a). Analysis of raffinose family oligosaccharide pathway in pea seeds with contras ting carbohydrate composition. Plant Physiol 127: 1764-1772.
  • Peterbauer T, Mucha J, Mach L and Richter A (2002b). Chain elongation of raffinose in pea seeds: isolation, characterization, and molecular cloning of a multifunctional enzyme catalyzing the synthesis of s tachyose and verbascose. J Biol Chem 277: 194-200.
  • Peters S, Egert A, S tieger B and Keller F (2010). Functional identification of Arabidopsis ATSIP2 (At3g57520) as an alkaline α-galactosidase with a subs trate specificity for raffinose and an apparent sink-specific expression pattern. Plant Cell Physiol 51: 1815-1819.
  • Polowick PL, Baliski DS, Bock C, Heather R and Fawzy G (2009). Over-expression of α-galactosidase in pea seeds to reduce raffinose oligosaccharide content. Botany/Botanoque 87: 526-532.
  • Qiu D, Vuong T, Valliyodan B, Shi H, Guo B, Shannon JG and Nguyen HT (2015). Identification and characterization of a s tachyose synthase gene controlling reduced s tachyose content in soybean. Theor Appl Genet 128: 2167-2176.
  • Quemener B and Brillouet J (1983). Ciceritol, a pinitol digalactoside form seeds of chickpea, lentil and white lupin. Phytochem 22: 1745-1751.
  • Rao VS and Vakil UK (1983). Effects of gammairradiation on flatulence causing oligosaccharides in green gram (Phaseolus aureus). J Food Sci 48: 1791-1795.
  • Reddy NR and Salunkhe DK (1980). Changes in oligosaccharides during germination and cooking of black gram and fermentation of black gram/ rice blend.Cereal Chem 57: 356-360.
  • Reddy NR, Pierson MD, Sathe SK and Salunkhe DK (1984). Chemical, nutritional and physiological aspects of dry bean carbohydrates: a review. Food Chem 13: 25-68.
  • Rehman Z and Shah WH (2005). Thermal heat processing effects on antinutrients, protein and s tarch diges tibility of food legumes. Food Chem 91: 327-331.
  • Richter A, Hoch G, Puschenreiter M, Mayer U and Peterbauer T (2000). The role of s tachyose synthase in the oligosaccharide metabolism of legume seeds. In: Seed biology: Advances and applications, Black M, Bradford KJ and Vásquez- Ramos J (eds.), CAB Int, Wallingford, pp: 75-84.
  • Roberfroid MB (1999). Concepts in Functional Foods: The Case of Inulin and Oligofructose. J Nutr 129: 1398S-1401S.
  • Roberfroid MB, Van Loo JAE and Gibson GR (1998). The bifidogenic nature of chicory inulin and its hydrolysis products. J Nutr 128: 11-19.
  • Saini HS and Knights EJ (1984). Chemical cons titution of s tarch and oligosaccharide components of ‘desi’ and ‘kabuli’ chickpea (Cicer arietinum) seed types. J Agric Food Chem 32: 940-944.
  • Sánchez-Mata MC, Cámara MM and Díez-Marqués C (1999). Effect of domes tic processes and water hardness on soluble sugars contents of chickpeas (Cicer arietinum L.). Food Chem 65: 331-338.
  • Sebas tian SA, Kerr PS, Pearls tein RW and Hitz WD (2000). Soybean germplasm with novel genes for improved diges tibility. In: Soy in animal nutrition, Drackley JK (eds.), Federation of Animal Science Societies, Savoy, pp: 56-74.
  • Sengupta S, Mukherjee S, Basak P and Majumder AL (2015) Significance of galactinol and raffinose family oligosaccharide synthesis in plants. Front Plant Sci. 6: 656.
  • Shewry PR and Halford NG (2002). Cereal seed s torage proteins: s tructures, properties and role in grain utilization. J Exp Bot 53: 947-958.
  • Shimelis EA and Rakshit SK (2007). Effect of processing on antinutrients and in vitro protein diges tibility of kidney bean (Phaseolus vulgaris L.) varieties grown in Eas t Africa. Food Chem 103: 161-172.
  • Smiricky MR, Grieshop CM, Albin DM, Wubben, JE, Gabert VM and Fahey GCJ (2002). The influence of soy oligosaccharides on apparent and true ileal amino acid diges tibilities and fecal consis tency in growing pigs. J Animal Sci 80: 2433-2441.
  • Sosulski FW, Elkowicz L and Reichert RD (1982). Oligosaccharides in eleven legumes and their air-classified protein and s tarch fractions. J Food Sci 47: 498-502.
  • Sprenger N and Keller F (2000). Allocation of raffinose family oligosaccharides to transport and s torage pools in Ajuga reptans: the roles of two dis tinct galactinol synthases. Plant J 21: 249-258.
  • Tahir M, Båga M, Vandenberg A and Chibbar RN (2012). An assessment of raffinose family oligosaccharides and sucrose concentration in genus Lens. Crop Sci 52: 1713- 1720.
  • Tahir M, Vandenberg A and Chibbar RN (2011). Influence of environment on seed soluble carbohydrates in selected lentil cultivars. J Food Compos Anal 24: 596-602.
  • Taji T, Ohsumi C, Iuchi S, Seki M, Kasuga M, Kobayashi M, Yamaguchi-Shinozaki K and Shinozaki K (2002). Important roles of droughtand cold-inducible genes for galactinol synthase in s tress tolerance in Arabidopsis thaliana. Plant J 29: 417-426.
  • Tanner W and Kandler O (1968). Myo-Inositol, a cofactor in the biosynthesis of s tachyose. Eur J Biochem 4: 233-239.
  • Tapernoux-Lüthi EM, Schneider T and Keller F (2007). The C-terminal sequence from common bugle leaf galactan:galactan galactosyltransferase is a non-sequence-specific vacuolar sorting determinant. FEBS Letters 581: 1811-1818.
  • Torres A, Frias J, Granito M and Vidal-Valverde C (2006). Fermented pigeon pea (C. cajan) ingredients in pas ta products. J Agric Food Chem 54: 6685-6691.
  • Ueda T, Coseo MP, Thomas JH and Obendorf RL (2005). A multifunctional galactinol synthase catalyzes the synthesis of fagopyritol A1 and fagopyritol B1 in buckwheat seed. Plant Sci 168: 681-690.
  • Upadhyay JK and Garcia VV (1988). Effect of soaking and cooking on reduction of oligosaccharides of cowpea (Vigna unguiculata (L) Walp). Philip J Food Sci & Tech 12: 21-28.
  • Urbano G, Lopez-Jurado M, Hernandez J, Fernandez M, Moreu MC, Frias J, Diaz-Pollan C, Prodanov M and Vidal-Valverde C (1995). Nutritional assessment of raw, heated, and germinated lentils. J Agric Food Chem 43: 1871-1877.
  • Van Barneveld RJ (1999). Unders tanding the nutritional chemis try of lupin (Lupinus spp.) seed to improve lives tock production efficiency. Nutr Res Rev 12: 203-230.
  • Veldman A, Veen WAG, Barug D and Van Paridon PA (1993).Effect of α-galactosides and α-galactosidase in feed on ileal piglet diges tive physiology. J Animal Physiol Animal Nutr 69: 57-65.
  • Viana PA, Tavares de Rezende S, Falkoski DL, Leite T, José IC, Moreira MA and Guimarães VM (2007). Hydrolysis of oligosaccharides in soybean products by Debaryomyces hansenii UFV-1 α-galactosidases. Food Chem 103: 331-337.
  • Viana SF, Guimarães VM, José IC, Oliveira MGA, Cos ta NMB, Barros EG, Alves MM and Rezende S (2005). Hydrolysis of oligosaccharides in soybean flour by soybean a-galactosidase. Food Chem 93: 665-670.
  • Vidal-Valverde C, Frias J, Hernández A, Martín- Alvarez PJ, Sierra I, Rodríguez C, Blazquez I and Vicente G (2003). Assessment of nutritional compounds and antinutritional factors in pea (Pisum sativum) seeds. J Sci Food Agric 83: 298-306.
  • Vidal-Valverde C, Frias J, Sotomayor C, Diaz-Pollan C, Fernandez M and Urbano G (1998). Nutrients and antinutritional factors in faba beans as affected by processing. Z Lebensm Unters Forsch 207: 140-145.
  • Vijayakumari K, Pugalenthi M and Vadivel V (2007). Effect of soaking and hydrothermal processing methods on the levels of antinutrients and in vitro protein diges tibility of Bauhinia purpurea L. seeds. Food Chem 103: 968- 975.
  • Wada K, Watabe J, Mizutani J, Suzuki H, Kiriku N, Hayakawa K and Yamaguchi C (1991). Effects of soybean oligosaccharides intake on fecal microflora, enzyme activity, ammonia and frequency of evacuation in elderly persons. Bifidus 4: 135-140.
  • Wang N, Hatcher DW, Gawalko EJ (2008). Effect of variety and processing on nutrients and certain anti-nutrients in field peas (Pisum sativum).Food Chem 111: 132-138.
  • Wang N, Hatcher DW, Toews R and Gawalko EJ (2009). Influence of cooking and dehulling on nutritional composition of several varieties of lentils (Lens culinaris). Food Sci Tech 42: 842-848.
  • Wang N, Hatcher DW, Tyler RT, Toews R and Gawalko EJ (2010). Effect of cooking on the composition of beans (Phaseolus vulgaris L.) and chickpeas (Cicer arietinum L.).Food Res Int 43: 589-594.
  • Wang N, Lewis MJ, Brennan JG and Wes tby A (1997). Effect of processing methods on nutrients and anti-nutritional factors in cowpea. Food Chem 58: 59-68.
  • Wang TL, Domoney C, Hedley CL, Casey R and Grusak MA (2003). Can we improve the nutritional quality of legume seeds? Plant Physiol 131: 886–891.
  • Wiggins HS (1984). Nutritional value of sugars and related compounds undiges ted in the small intes tine. Proc Nutr Soc 43: 69-75.
  • Yamaguishi CT, Sanada CT, Gouvêa PM, Pandey A, Woiciechowski AL, Parada JL and Soccol CR (2009). Biotechnological process for producing black bean slurry without s tachyose. Food Res Intern 42: 425-429.
  • Youssef MM, Hamza MA, Abdel-Aal MH, Shekib LA and El-Banna AA (1986). Amino acid composition and in vitro diges tibility of some Egyptian foods made from faba bean (Vicia faba L.). Food Chem 22: 225-233.
  • Zhao T, Martin D, Meeley RB and Downie B (2004). Expression of the maize galactinol synthase gene family: (II) Kernel abscission, environmental s tress and myo-inositol influences accumulation of transcript in developing seeds and callus cells. Physiol Plant 121: 647-655.
  • Zuo Y, Fahey GC, Merchen NR and Bajjalieh NL (1996). Diges tion responses to low oligosaccharide soybean meal by ileally-cannulated dogs. J Anim Sci 74: 2441-2449.
There are 161 citations in total.

Details

Primary Language English
Journal Section Articles
Authors

Udhaya Kannan This is me

Roopam Sharma This is me

Manu P. Gangola This is me

Ravindra N. Chıbbar This is me

Publication Date January 30, 2018
Published in Issue Year 2018 Volume: 4 Issue: 1

Cite

APA Kannan, U., Sharma, R., Gangola, M. P., Chıbbar, R. N. (2018). Improving Grain Quality in Pulses: S trategies to Reduce Raffinose Family Oligosaccharides in Seeds. Ekin Journal of Crop Breeding and Genetics, 4(1), 70-88.
AMA Kannan U, Sharma R, Gangola MP, Chıbbar RN. Improving Grain Quality in Pulses: S trategies to Reduce Raffinose Family Oligosaccharides in Seeds. Ekin Journal. January 2018;4(1):70-88.
Chicago Kannan, Udhaya, Roopam Sharma, Manu P. Gangola, and Ravindra N. Chıbbar. “Improving Grain Quality in Pulses: S Trategies to Reduce Raffinose Family Oligosaccharides in Seeds”. Ekin Journal of Crop Breeding and Genetics 4, no. 1 (January 2018): 70-88.
EndNote Kannan U, Sharma R, Gangola MP, Chıbbar RN (January 1, 2018) Improving Grain Quality in Pulses: S trategies to Reduce Raffinose Family Oligosaccharides in Seeds. Ekin Journal of Crop Breeding and Genetics 4 1 70–88.
IEEE U. Kannan, R. Sharma, M. P. Gangola, and R. N. Chıbbar, “Improving Grain Quality in Pulses: S trategies to Reduce Raffinose Family Oligosaccharides in Seeds”, Ekin Journal, vol. 4, no. 1, pp. 70–88, 2018.
ISNAD Kannan, Udhaya et al. “Improving Grain Quality in Pulses: S Trategies to Reduce Raffinose Family Oligosaccharides in Seeds”. Ekin Journal of Crop Breeding and Genetics 4/1 (January 2018), 70-88.
JAMA Kannan U, Sharma R, Gangola MP, Chıbbar RN. Improving Grain Quality in Pulses: S trategies to Reduce Raffinose Family Oligosaccharides in Seeds. Ekin Journal. 2018;4:70–88.
MLA Kannan, Udhaya et al. “Improving Grain Quality in Pulses: S Trategies to Reduce Raffinose Family Oligosaccharides in Seeds”. Ekin Journal of Crop Breeding and Genetics, vol. 4, no. 1, 2018, pp. 70-88.
Vancouver Kannan U, Sharma R, Gangola MP, Chıbbar RN. Improving Grain Quality in Pulses: S trategies to Reduce Raffinose Family Oligosaccharides in Seeds. Ekin Journal. 2018;4(1):70-88.