Cytoplasmic Genome Prediction in Cucumber (Cucumis sativus L.) Hybrid Variety Breeding

Year 2023, Volume: 9 Issue: 1, 19 - 23, 01.02.2023

Leyla Öztürk Akar Osman Gülsen Sinan Zengin G. Elif Vural

Abstract

Breeding studies in Cucurbitaceae species take a long time. It has become necessary to shorten the time and support
traditional breeding methods with modern biotechnological methods to get qualified domestic cucumber varieties.
Cytoplasmic genome prediction within the scope of molecular-based breeding is a very important application. To increase
heterosis in test crosses, reciprocal ‘double way’ crosses can be made as well as single crosses. Cytoplasmic organelles
‘plastid and mitochondria’ are considered to be different from each other between individuals and reciprocal crosses are
made based on this idea. However it significantly increases the labor. In this study, 4 plastid genome regions (rbcL, psbtrnS, trnHK, trnSt) located within non-conserved regions therefore expected to be variable of 50 donor genotypes were
sequenced, analyzed and their cytoplasmic genome prediction was estimated. A total of 6300 bp including four plastid
regions indicated no polyfmorphism and all sequences were identical among the 50 donor genotypes analyzed. This may
imply no cytoplasmic organelle variation. In conclusion, reciprocal crosses were excluded from our breeding studies. So
cytoplasmic genome prediction can provide rapidity and savings in breeding by eliminating unnecessary reciprocal test
crosses.

Keywords

Breeding, Cucumber, Cucumis sativus L., Cytoplasmic Genome Prediction, Double Haploid, Hybrid Variety Breeding., Breeding, Cucumber, Cucumis sativus L., Cytoplasmic Genome Prediction, Double Haploid, Hybrid Variety Breeding.

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