Accelerated Pepper Breeding Using Molecular Markers and Doubled Haploidy Technique

Year 2023, Volume: 9 Issue: 2, 141 - 149, 01.08.2023

Mesut Nar Hande Nur Albezirgan1 Burcu D Emirkaya Hakan Fidan Hakan Aktaş Ş. Şebnem Ellialtıoğlu

Abstract

Hatay pepper is preferred in terms of quality characteristics e.g. hot, fresh and suitable for dried consumption, but it
does not contain any disease resistance genes. Within the scope, we established the study is aiming to develop resistant
hybrid varieties to TSWV is causing high yield and quality losses in pepper production areas. For this purpose, studies
are carried out to obtain inbreed resistant lines in a short time, a Tsw gene found in Capsicum chinense, which provides
resistance to TSWV, was combined with Hatay pepper lines by crossbreeding. First of all, 20 of the Hatay pepper lines
from the gene pool were selected according to weighted grading criteria, then the number was reduced to 4. In C. chinense
lines, molecular screening was performed with SCAC568 primers and individuals with Tsw gene were determined from
donor parents and crosses. After then, backcrosses to Hatay peppers were conducted and BC1F1 was generated. Their
seed samples were sown from them and brought until the first true leaves during the transition from seed to seedling
stage. Meanwhile, molecular analyses were applied to find resistant individuals with Tsw gene. The homozygous and
heterozygous plants were planted in a greenhouse and used in anther culture study. Whole study takes 22 months from
the initiation of hybridization to the emergence of androgenic embryos and acclimatization to external conditions and
development of DH seeds. Here, these findings are presented as a case study in biotechnology and the combined techniques
are an indispensable part of accelerated breeding processes.

Keywords

Anther culture, Capsicum spp., Tsw gene, Hatay pepper, molecular marker

References

• Abak K, Pochard E and Dumas De Vaulx R, (1982). Transmission of resistance to Phytophthora capsici on root and stems of pepper plants: Study of doubled haploid lines issued from the cross “PM 217 x Yolo Wonder” through anther culture. Capsicum Newsletter 1:62-63.
• Alremi F, Taşkın H, Sönmez K, Büyükalaca S and Ellialtıoğlu Ş, (2014). Biber (Capsicum annuum L.)’de genotip ve besin ortamının anter kültürüne etkileri. Türk Tarım ve Doğa Bilimleri Dergisi 1 (2): 108-116, https://doi.org/10.30910/turkjans.160702 (in Turkish).
• Anonymous, (2020). TÜİK kurumsal. https://data. tuik.gov.tr/Bulten/Index?p=Bitkisel-Uretim istatistikleri 2020 33737 (Erişim Tarihi: 23.02.2021)
• Atasoy D, Baktemur G and Taşkın H, (2021). Bazı biber (Capsicum annuum L.) genotiplerinin anter kültürü performanslarının belirlenmesi. Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi 31(2):282-293. (in Turkish)
• Bat H, Shidfar M, Çömlekçioğlu N and Ellialtıoğlu ŞŞ, (2020). In vitro androgenesis in pepper and the affecting factors on success: I. Carbon source and concentrations. Biotech Studies, 29(2):62-68.
• Çömlekçioğlu N and Ellialtıoğlu ŞŞ, (2018). Review on the research carried out on in vitro androgenesis of peppers (Capsicum annuum L.) in Turkey. Res. J. Biotechnol., 13(6):75-84.
• Doyle JJ and Doyle JL, (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bulletin, 19:11-15.
• Dumas De Vaulx R, Pochard E and Chambonnet B, (1982). Distribution of TMVpo susceptible and resistant doubled haploid lines from anther culture of heterozygous L+ /L1 hybrids. Capsicum Newsletter 1:52-53.
• Ellialtıoğlu Ş, Kaplan F and Abak K, (2001). The effect of carrot extract and activated charcoal on the androgenesis of pepper. In Proceedings XIth EUCARPIA Meeting on Genetics and Breeding of Capsicum & Eggplant, (Vol.9, p.13), Antalya- Türkiye
• FAOSTAT, (2020). Crop Statistics http://www.fao. org/faostat/en/#data/QC/visualize. (Accessed 09.01.2020)
• German TL, Ullman DE and Moyer JW, (1992). Tospoviruses: Diagnosis. Molecular biology, phylogeny and vector relationships. Annu. Rev. Phytopathol. 30:335-348.
• Griep RA, Prins M, van Twisk C, Keller HJHG, Kerschbaumer RJ, Kormelink R, Goldbach RW and Schots A, (2000). Application of phage display in selecting tomato spotted wilt virusspecific single-chain antibodies (scFvs) for sensitive diagnosis in ELISA. Phytopathology, 90(2):183-190. doi:10.1094/phyto.2000.90.2.183
• Heberle-Bors E, (1985). In vitro haploid formation from pollen: A critical review. Theor. Appl. Genet. 71: 361-374.
• Keleş D, Pınar H, Ata A, Taşkın H, Yıldız S and Büyükalaca S, (2015). Effect of pepper types on obtaining spontaneous doubled haploid plants via anther culture. Hort Science, 50(11):1671-1676.
• Nar M, Albezirgan HN, Demirkaya B, Fidan H, Aktaş H and Ellialtıoğlu ŞŞ, (2022). Accelerated pepper breeding studies with MAS and androgenesis techniques. IV. International Plant Breeding Congress Plant Breeding for the Future: From Local to Global, Antalya Türkiye, 21-25 November 2022, Proceedings pp: 250-259.
• Öntürk G and Çürük S, (2019). Hatay’ın farklı ilçelerinde yetiştirilen biber köy popülasyonlarında bitki ve meyve özellikleri. Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi, 29 (4):689-701. doi: 10.29133/yyutbd. 639261. (in Turkish)
• Pochard E, Dumas De Vaulx R and Florent A, (1983). Linkage between partial resistance to CMV and susceptibility to TMV in the line “Perennial”. Capsicum Newsletter 2:34-35.
• Sönmez K, Ellialtıoğlu ŞŞ and Oğuz A, (2015). Yerel sofralık domates gen kaynaklarının değerlendirilmesinde tartılı derecelendirme yönteminin kullanımı. Tarım Bilimleri Araştırma Dergisi (TABAD), 8(1):14-19. (in Turkish)
• Şevik M, (2014). Domates lekeli solgunluk virüsü (TSWV)’nün tarımsal ürünlerde meydana getirdiği ekonomik kayıplar. Harran Tarım ve Gıda Bilimleri Dergisi, 15(1):35-42.
• https://dergipark.org.tr/en/pub/ harranziraat/ issue/18434/194124. (in Turkish)
• UPOV (2021). https://www.upov.int/ (Accessed: 21.12.2022). Vagera J, (1990). Pepper (Capsicum spp.): In Vitro induction of haploids. In: Biotechnology in agriculture and forestry, Vol:12, Haploids in Crop Improvement I (ed. by Y.P.S. Bajaj), Springer- Verlag Berlin Heidelberg, pp 374-392.
• Vural G, Arı E, Zengin S and Ellialtıoğlu ŞŞ, (2019). Development of androgenesis studies on eggplant (Solanum melongena L.) in Türkiye from past to present. In book: Sustainable Crop Production (Ed: Dr. Mirza Hasanuzzaman) ISBN 978-1-78985- 318-6 doi: 10.5772/intechopen.88299. pp: 1-27.