Determination of Heat and Drought Tolerant Lines in Segregating Populations Produced by Interspecific Crosses in Eggplant

Year 2023, Volume: 9 Issue: 2, 81 - 90, 01.08.2023

Esra Cebeci H. Filiz Boyacı Yıldız Doğan Laura Toppıno Giuseppe L. Rotıno

Abstract

Nowadays abiotic stresses originated by climate change are one of the main factors causing reductions to the agricultural production. Heat and drought are the most prominent abiotic stress factors affecting both eggplant production worldwide and food security. Although eggplant is known as more tolerant to these stresses compared to other
vegetables and solanaceous crops, its quality and yield suffers from severe stress conditions. In this research, 256
F2 plants developed from the interspecific cross between the wild relative of Solanum insanum L. and the pure line
(BATEM-TDC47) from Batı Akdeniz Agricultural Research Institute, (BATEM) eggplant gene pool were used as
plant materials. Seedlings at 3-4 true leaves stage bred in three-liter pots filled with a 1:1 mixture of peat and perlite
were subjected to drought stress test to this end, a 75% deficit irrigation was applied to the plants, while control plants
were irrigated with the required amount to recover the 100% of ETp as appropriate management strategy. The stress
symptoms of plants were determined by morphological and chemical analyses. Plant heights were measured on the
25th day of the experiment and visual evaluation stress symptoms was observed according to the 0-5 scale. Morphological observations, MDA (malondialdehyde) and proline content of selected plants were performed to confirm their
tolerance levels to heat and drought. Following the drought test, 100 F2 lines, which were selected as drought tolerant,
were transferred to the greenhouse for determination of heat tolerant individuals.

Keywords

biotic stress, drought, eggplant, heat, MDA, proline

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