Diversity Analysis for Drought Tolerance in Pearl Millet Inbred Lines using SSR Markers

Year 2024, Volume: 10 Issue: 1, 36 - 51, 01.02.2024

Jagdeep Sıngh Ashok K Chhabra Rishi K Behl Akshay K Vats Pooja Malık

Abstract

50 pearl millet genotypes (inbred lines) were grown in RBD design at two contrasting locations in Haryana, India
including one at CCSHAU Hisar and another at RRS, Bawal. Genomic DNA was isolated from leaves of 2-3 weeks old
plants using CTAB extraction method. The SSR diversity data was used to determine the genetic relationship among the
fifty genotypes. A similarity matrix was first made using SIMQUAL subprogram of software. The dendrogram was then
constructed based on the simple matching coefficient using SAHN sub-program. The SAHN sub-program uses UPGMA
algorithm to perform cluster analysis. Out of 50 SSRs used for identification of single marker analysis 9 SSRs were
found polymorphic for further checking their behaviour on known drought tolerant (HTP 93-37, HTP 03/13-901-1) and
drought-sensitive inbreeds (HM S33B, HMS 42B) and were used for amplification of DNA. SSRs showed amplification
for all genotypes and thus confirmed in other genotypes for the study of drought in pearl millet genotypes. Primers
amplified a total of 237 alleles which varied from 2 to 8 with a mean of 4.54 alleles per locus. The overall size of PCRamplified products ranged from 140 bp (PSMP 2271) to 810 bp (ICMP 10). Polymorphic information content (PIC) value
ranged from 0.326 (PSMP2201) to 0.89 (XCUMP 009) with an average of 0.579. 50 Inbred lines based on SSR marker
polymorphism data were resolved into 11 diverse clusters two genotypes HTP 9337 and HMS 43B were failed to fall
into any cluster. Based on SSR markers and morphophysiological data two inbred lines (HTP 93-37, HTP 03/13-901-1)
appeared drought tolerant which may be used for hybrid development program.

Keywords

Inbred, drought tolerant, polymorphism, genotypes, SSR markers

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