Determination of Resistance to Tomato Yellow Leaf Curl Virus by Molecular Methods in Pink Beef Tomatoes

Year 2024, Volume: 10 Issue: 1, 52 - 58, 01.02.2024

Mine Bulut Sevinç Tekin Elif Ürün Köksalan Veysel Aras

Abstract

In both public and private tomato breeding projects, marker assisted selection (MAS) for disease resistance is frequently
used. In tomato molecular breeding programs, the development and application of molecular markers have been
extensively pursued, particularly for disease resistance to enable the selection of a single resistance gene or a combination
of multiple resistance genes. Tomato Yellow Leaf Curl Virus (TYLCV) is one of the most dangerous viruses affecting
tomato production and growth worldwide. Using resistant cultivars is the most effective and eco-friendly way to combat
TYLCV. In this study, the TYLCV was tested against 155 genotypes of pink beef tomatoes utilizing the MAS (MarkerAssisted Selection) technique. Resistance against TYLCV was determined with the SCAR (P6-25) primer developed in
connection with the Ty-3 gene. 42 pink tomato genotypes were determined to be sensitive (rr), 99 to be heterozygous
resistant (Rr), and 8 to be homozygous resistant (RR) to TYLCV as a consequence of MAS testing. Furthermore, no
molecular marker was found in any of the six pink beef tomato genotypes. These findings suggested that P6-25 (SCAR)
primers could be used successfully in breeding studies to identify disease resistance.

Keywords

Tomato, MAS, TYLCV, resistance breeding

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