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Resistance levels and detoxification enzymes against some acaricides in populations of Tetranychus urticae Koch (Acari:Tetranychidae) collected from tomato greenhouses in central district of Isparta province

Year 2014, Volume: 4 Issue: 3, 185 - 195, 23.10.2014
https://doi.org/10.16969/teb.99216

Abstract

In this study, the resistance levels of Tetranychus urticae Koch (Acari:Tetranychidae) populations collected from the greenhouses in the central district of the province of Isparta, Deregumu village, in 2014 against abamectin, spiromesifen and hexythiazox were determined by bioassay and biochemical methods. LC50 values of T. urticae populations were found through leaf disk method by using spray tower. The resistance levels of T. urticae populations was determined as between 8.36-25.26 folds against abamectine, 8.16-22.82 folds against spiromesifen and 8.85-11.76 folds against hexythiazox. Enzymes of glutathione S-transferase (GST) and monooxygenase (P450) in the T. urticae popluations were determined by using the kinetic method; and the enzyme of esterase was determined by using the electrophoresis and kinetic methods. The levels of esterase, glutathione S-transferase (GST), and monooxygenases (P450) in the populations of T. urticae were 10.80-22.60, 2.56-2.78 and 0.0020-0.0040 mOD min-1 mg-1 protein, respectively. As a result, it was concluded that the esterase and P450 enzymes can be effective, but GST enzyme is not effective in the development of resistance against three acaricides in Tetranychus urticae populations collected from tomato greenhouses.

Isparta ili merkez ilçesinde domates seralarından toplanan Tetranychus urticae Koch (Acari: Tetranychidae) popülasyonlarının bazı akarisitlere karşı direnç düzeyleri ve detoksifikasyon enzimleri

Year 2014, Volume: 4 Issue: 3, 185 - 195, 23.10.2014
https://doi.org/10.16969/teb.99216

Abstract

Bu çalışmada, Isparta ili Merkez ilçe Deregümü köyü domates seralarından 2014 yılında toplanan T. urticae popülasyonlarının abamectin, spiromesifen ve hexythiazox'a karşı direnç düzeyler bioassay ve biyokimyasal yöntemlerle belirlenmiştir. T. urticae popülasyonlarının LC50 değeri ilaçlama kulesi kullanılarak yaprak disk metodu ile bulunmuştur. T. urticae popülasyonlarında abamectine’e karşı 8.36-25.26 kat; spiromesifen'e karşı 8.16-22.82 kat ve hexythiazox'a karşı 8.85- 11.76 kat arasında direnç düzeyleri belirlenmiştir. T. urticae popülasyonlarında dirençle bağlantılı detoksifikasyon enzimlerinden esteraz enzimi elektroforez ve kinetik yöntemlerle, GST ve P450 enzimleri ise kinetik yöntemle belirlenmiştir. T. urticae popülasyonlarının esteraz, GST ve P450 seviyeleri sırasıyla 10.80-22.60, 2.56-2.78 ve 0.0020-0.0040 mOD min-1 mg-1 protein arasında değişmiştir. Sonuç olarak domates seralarından toplanan T. urticae popülasyonlarında üç akarisite karşı gelişen dirençte esteraz ve P450 enzimlerinin etkili olabileceği, ancak GST enziminin ise direnç gelişiminde etkili olmadığı görüşüne varılmıştır.

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Details

Primary Language English
Journal Section Original Article
Authors

Sibel Yorulmaz Salman

Bengi Kaplan This is me

Publication Date October 23, 2014
Published in Issue Year 2014 Volume: 4 Issue: 3

Cite

APA Yorulmaz Salman, S., & Kaplan, B. (2014). Resistance levels and detoxification enzymes against some acaricides in populations of Tetranychus urticae Koch (Acari:Tetranychidae) collected from tomato greenhouses in central district of Isparta province. Türkiye Entomoloji Bülteni, 4(3), 185-195. https://doi.org/10.16969/teb.99216
AMA Yorulmaz Salman S, Kaplan B. Resistance levels and detoxification enzymes against some acaricides in populations of Tetranychus urticae Koch (Acari:Tetranychidae) collected from tomato greenhouses in central district of Isparta province. Türkiye Entomoloji Bülteni. December 2014;4(3):185-195. doi:10.16969/teb.99216
Chicago Yorulmaz Salman, Sibel, and Bengi Kaplan. “Resistance Levels and Detoxification Enzymes Against Some Acaricides in Populations of Tetranychus Urticae Koch (Acari:Tetranychidae) Collected from Tomato Greenhouses in Central District of Isparta Province”. Türkiye Entomoloji Bülteni 4, no. 3 (December 2014): 185-95. https://doi.org/10.16969/teb.99216.
EndNote Yorulmaz Salman S, Kaplan B (December 1, 2014) Resistance levels and detoxification enzymes against some acaricides in populations of Tetranychus urticae Koch (Acari:Tetranychidae) collected from tomato greenhouses in central district of Isparta province. Türkiye Entomoloji Bülteni 4 3 185–195.
IEEE S. Yorulmaz Salman and B. Kaplan, “Resistance levels and detoxification enzymes against some acaricides in populations of Tetranychus urticae Koch (Acari:Tetranychidae) collected from tomato greenhouses in central district of Isparta province”, Türkiye Entomoloji Bülteni, vol. 4, no. 3, pp. 185–195, 2014, doi: 10.16969/teb.99216.
ISNAD Yorulmaz Salman, Sibel - Kaplan, Bengi. “Resistance Levels and Detoxification Enzymes Against Some Acaricides in Populations of Tetranychus Urticae Koch (Acari:Tetranychidae) Collected from Tomato Greenhouses in Central District of Isparta Province”. Türkiye Entomoloji Bülteni 4/3 (December 2014), 185-195. https://doi.org/10.16969/teb.99216.
JAMA Yorulmaz Salman S, Kaplan B. Resistance levels and detoxification enzymes against some acaricides in populations of Tetranychus urticae Koch (Acari:Tetranychidae) collected from tomato greenhouses in central district of Isparta province. Türkiye Entomoloji Bülteni. 2014;4:185–195.
MLA Yorulmaz Salman, Sibel and Bengi Kaplan. “Resistance Levels and Detoxification Enzymes Against Some Acaricides in Populations of Tetranychus Urticae Koch (Acari:Tetranychidae) Collected from Tomato Greenhouses in Central District of Isparta Province”. Türkiye Entomoloji Bülteni, vol. 4, no. 3, 2014, pp. 185-9, doi:10.16969/teb.99216.
Vancouver Yorulmaz Salman S, Kaplan B. Resistance levels and detoxification enzymes against some acaricides in populations of Tetranychus urticae Koch (Acari:Tetranychidae) collected from tomato greenhouses in central district of Isparta province. Türkiye Entomoloji Bülteni. 2014;4(3):185-9.