The Effect of N-butyl-2-Cyanoacrylate on Wound Healing in Head Neck Region

Year 2019, Volume: 9 Issue: 1, 18 - 24, 19.03.2019

Zeliha Kapusuz Gencer , Mahmut Özkırış Levent Saydam Yusuf Kenan Dağlıoğlu Muzaffer Gencer Sait Polat

https://doi.org/10.32448/entupdates.425951

Abstract

OBJECTIVE

To
compare the histological regeneration characteristics of open wound
on head neck by a topical
antiseptic
solution (Batticon®
solution 5%; Adeka, Samsun, Turkey)
or
a tissue adhesive (N-butyl-2-cyanoacrylate, Glubran 2, GEM Company,
Italy) in experimentally created rabbit auricular open wound.

MATERIAL AND
METHOD

Twenty-eight
female rabbits (New Zealand genus), weighing 3 to 4 kg, were handled
daily for 2 weeks prior to surgical manipulation to condition them to
behavior testing. The control group consisted of seven rabbits. The
21 rabbits were randomly divided into three
groups based on the harvesting day. In control group a segment of ear
auricula was resected and sent to histological examination for
comparison with the study group findings. In the study group both
auricular skin of each animal was exposed and 1x1 cm epidermal
resection area was created open wound in rabbit ears that is to be a
model for open tissue injury and applied
as a
topical
antiseptic
solution
(Batticon
® solution 5%; Adeka, Samsun, Turkey)
on one side and by application of tissue adhesive (Glubran 2, GEM
Company, Italy) on the other side. Following this application at
2^nd,
4^th
and 6th days the animals were harvested and histological regeneration
characteristics of open wound examined by electron microscopy and
compared to the control group’s histological findings.

RESULTS

Electron
microscopic examination of open wound at consecutive 2^nd,
4^th
and 6^th
days revealed increased regeneration findings in tissue adhesive
group when compared to a antiseptic
solution
group.

CONCLUSION

Based
on the findings of this animal study we conclude that
N-butyl-2-cyanoacrylate
is an appropriate material for open wound on head and neck region.

Keywords

Open wound, N-butyl-2-cyanoacrylate, regeneration

References

• REFERENCES
• Reference 1. McKinley SH, Yen MT (2005) Octyl-2-cyanoacrylate tissue adhesive in external dacryocystorhinostomy. Ophthal Plast Reconstr Surg 21(3):197–200Reference 2. Ardis AE. U.S. Patents No. 2467926 and 2467927(1949). Reference 3. Coover HW, Joyner FB,Shearer Jr NH, Wicker Jr TH. Chemistry and performance of cyanoacrylate adhesive. J Soc Plast Eng. 1959;15:413-17.Reference 4. Toriumi DM, Raslan WF, Friedman M, Tardy E. Histotoxicity of cyanoacrylate tissue adhesives. A comparative study. Arch Otolaryngol Head Neck Surg. 1990;116:546-50. Reference 5. Straatsma BR, Allen RA, Hale PN, Gomez R. Experimental studies employing adhesive compounds in ophthalmic surgery. Trans Am Acad Ophthalmol Otolaryngol. 1963;67:320–34. Reference 6. Giray CB, Sungur A, Atasever A, Araz K. Comparasion of silk sutures and n-butyl-2-cyanoacrylate on the healing of skin wounds. A pilot study. Aust Dent J. 1995;40:43-5.Reference 7. Weber SC, Chapman MW. Adhesives in orthopedic surgery a review of the literature and in vitro bonding strengths of bone-bonding agents. Clin Orthop. 1984;191249-56. Reference 8. Shermak MA, Wong L, Inoue W, Crain BJ, Im MJ, Chao EY, et al. Fixation of the craniofacial skeleton with butyl-2-cyanoacrylate and its effect on histotoxicity and healing. Plast Reconstr Surg. 1998;102:309-18. Reference 9. Pruitt BA, Jr, McManus AT, Kim SH, Goodwin CW. Burn wound infections: current status. World J Surg. 1998;22:135–145. Reference 10. Murray CK. Infections in burns. J Trauma. 2007;62:73.Reference 11. Cheadle WG, Turina M. Infection and organ failure in the surgical patient: a tribute to seminal contributions by Polk HC Jr. Am J Surg. 2005;190:173–177.Reference 12. Sebeny PJ, Riddle MS, Petersen K. Acinetobacter baumannii skin and soft-tissue infection associated with war trauma. Clin Infect Dis. 2008;47:444–449. Reference 13. Evans RP. Surgical site infection prevention and control: an emerging paradigm. J Bone Joint Surg Am. 2009;91:2–9. Reference 14. Neubauer T, Bayer GS, Wagner M. Open fractures and infection. Acta Chir Orthop Traumatol Cech. 2006;73:301–312. Reference 15. Broex EC, van Asselt AD, Bruggeman CA, van Tiel FH. Surgical site infections: how high are the costs? J Hosp Infect. 2009;72:193–201. Reference 16. Kawai K, Larson BJ, Ishise H, Carre AL, Nishimoto S, Longaker M, Lorenz HP.Calcium-based nanoparticles accelerate skin wound healing. PLoS One. 2011;6(11):e27106. Epub 2011 Nov 2Reference 17. Jayaraj KK, Jayachandran E, Gridhar B, Nair R, Jayakandan M, Kathiravan M, Srinivas GMl. Formulation and evaluation of povidone iodine liquid anti-dandruff shampoo. J Pharm Sci& Res 2009; 1: 108-11.Reference 18. Pérez M, Fernandez I, Márquez D, Bretaña RMG. Use of n-butyl-2-cyanoacrylate in oral surgery: biological and clinical evaluation. Artif Organs. 2000;24:241-43. Reference 19. Al-Belasy FA, Amer MZ. Hemostatic effect of n-butyl-2-cyanoacrylate (histoacryl) glue in warfarin-treated patients undergoing oral surgery. J Oral Maxillofac Surg. 2003;61:1405-09. Reference 20. Dôner F, Sari I. Facial wound closure with butyl 2-cyanoacrylate. J Basic Clin Physiol Pharmacol. 2003;14:359-71. Reference 21. Camacho-Alonso F, Lopez-Jornet P, Bermejo- Fenoll A. Effects of scalpel (with and without tissue adhesive) and cryosurgery on wound healing in rat tongues. Oral Surg Oral Med Oral Pathol Radiol Endod. 2005;100:58-63. Reference 22. Turkaslan T, Ozcan H, Dayicioglu D, Ozsoy Z. Use of adhesives in cleft palate surgery: a new flap fixation technique. J Craniofac Surg. 2005;16:719-22. Reference 23. Reece TB, Maxey TS, Kron IL. A prospectus on tissue adhesives. Am J Surg. 2001;182:40S;4S. Reference 24. Choi HB, Kim BY, Huh JY, Lee SH, Zhu SJ, Jung JH, et al. Microneural anastomosis using cyanoacrylate adhesives. Int J Oral Maxillofac Surg. 2004;33:777;80. Reference 25. Caroli A, Marcuzzi A, Limontini S, Maiorana A. An experimental study of a cyanoacrylate biological adhesive in view of its use in the fixation of various fractures of the fingers. Ann Chir Main Memb Super. 1997;16:138-45. Reference 26. Gonzalez E, Orta J, Niemshik L, Galera R, Onay R, Rojas O. Ethyl-2-cyanoacrylate fixation of the cranial bone flap after craniotomy. Surg Neurol. 2000;53:288-89. Reference 27. Ivana L Romero, João B N S Malta, Cely B Silva, Lycia M J Mimica, Kaz H Soong, and Richard Y Hida. Antibacterial properties of cyanoacrylate tissue adhesive: Does the polymerization reaction play a role?Indian J Ophthalmol. 2009 Sep-Oct; 57(5): 341–344. Reference 28. Eiferman RA, Snyder JW. Antibacterial effect of cyanoacrylate glue. Arch Ophthalmol. 1983;101:958–60.Reference 29. Chen WL, Lin CT, Hsieh CY, Tu IH, Chen WY, Hu FR.Comparison of the bacteriostatic effects, corneal cytotoxicity, and the ability to seal corneal incisions among three different tissue adhesives. Cornea. 2007 Dec;26(10):1228-34. Reference 30. Sharma A, Kaur R, Kumar S, Gupta P, Panda V S, Patnaik B, et al. Fibrin glue versus N-butyl-2-cyanoacrylate in corneal perforations. Ophthalmology. 2003;110:291–8. Reference 31. Refojo MF, Dohlman CH, Ahmad B, Carrol JM, Allen JC. Evaluation of adhesives for corneal surgery. Arch Ophthalmol. 1968;80:645–56. Reference 32. Goktas N, Karcioglu O, Coskun F, Karaduman S, Menderes A. Comparison of tissue adhesive and suturing in the repair of lacerations in the emergency department. Eur J Emerg Med. 2002; 9:155-8. Reference 33. Pelissier P, Casoli V, Le Bail B, Martin D, Baudet J. Internal use of n-butyl 2-cyanoacrylate (Indermil) for wound closure: an experimental study. Plast Reconstr Surg. 2001;108:1661-6.