Evaluation of salivary tumour necrosis factor–alpha in patients with recurrent aphthous stomatitis

Year 2018, Volume: 52 Issue: 3, 157 - 161, 01.09.2018

Shruthi Hegde , Vidya Ajila Subhas Babu Suchetha Kumari Harshini Ullal Ananya Madiyal

Abstract

DOI: 10.26650/eor.2018.543

Purpose

Present study was undertaken to evaluate
and compare the salivary levels of tumour necrosis factor alpha (TNF-α) in subjects with RAS, traumatic ulcers (TUs) in the oral mucosa
and in healthy controls.

Materials and Methods

Present study involved 90 participants of
which 30 subjects were diagnosed with RAS, 30 subjects with TUs and 30 healthy
controls grouped as group 1, group 2 and group 3 respectively. Unstimulated
saliva was collected from the subjects through ‘Spit Technique’ and the estimation of TNF-α was done by
enzyme linked immunosorbent assay.  The
data collected was statistically analysed.

Results

Salivary level of TNF-α was
significantly higher in RAS patients than in patients with TUs and healthy
controls. Difference between the Salivary TNF-α
level in our study groups were statistically significant (p<0.001).

Conclusion

Present study suggests that saliva is a
convenient and ideal medium for the detection of TNF-α.  Statistically significant difference in the
level of salivary TNF-α between the RAS and TUs subjects as
well as controls suggests the significant contribution of TNF-α in pathogenesis of RAS.

Keywords

Recurrent aphthous stomatitis; tumour necrosis factor-alpha; saliva; oral mucosa; traumatic ulcer

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