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The assessment of new bone formation induced by unfocused extracorporeal shock wave therapy applied on pre-surgical phase of distraction osteogenesis

Year 2019, Volume: 53 Issue: 3, 125 - 131, 01.10.2019

Abstract

Purpose: This study aims to evaluate the effects of extracorporeal shock wave therapy applied before and/or immediately after the osteotomy on the maturation during the consolidation phase.
Materials and Methods: 21 female New Zealand rabbits were used in the study. Subjects were divided randomly into three groups: Control (Distraction without ESWT), A (Distraction +ESWT After Osteotomy), AB (Distraction+ESWT After and Before Osteotomy). ESWT (500 pulses, 5 Hz, 0.19 mJ/mm2 energy flux density) was applied to group A and group AB after 5, 12 and 19 days after osteotomy and group AB only on days 7,14 and 21 before osteotomy. On the 28th day of the consolidation period, all subjects were sacrificed. Dual-energy x-ray absorptiometry (DEXA) was used to determine bone mineral density (BMD) and bone mineral content (BMC), and stereological methods were used to determine the new bone, connective tissue and neovascularization volumes.
Results: As a result of DEXA examinations made on the 1st and 4th week of consolidation, there was no significant difference between groups regarding BMD and BMC values. According to the results of stereological examination, when the connective tissue and new bone tissue were evaluated, higher values were observed in AB when compared to A, and in AB and A compared to the control group, but the differences are not statistically significant. There was no difference between the groups in terms of neovascularization.
Conclusion: ESWT in these parameters was not positively effective in bone maturation during consolidation when applied before osteotomy or both before and after osteotomy.

Supporting Institution

This study was supported by Project Management Office Coordinatorship of Ondokuz Mayıs University with PYO.DIS.1904.12.007 project code.

Project Number

PYO.DIS.1904.12.007

Thanks

We would like to thank the staff of Nuclear Medicine Department of Ondokuz Mayıs University, Faculty of Medicine for DEXA imaging.

References

  • 1. Raza H, Saltaji H, Kaur H, Flores-Mir C, El-Bialy T. Effect of Low-Intensity Pulsed Ultrasound on Distraction Osteogenesis Treatment Time: A Meta-analysis of Randomized Clinical Trials. J Ultrasound Med 2016;35(2):349-358.
  • 2. Zimmermann C, Thurmuller M, Troulis D, Perrott B, Rahn L, Kaban B. Histology of the porcine mandibular distraction wound. Int J Oral Maxillofac Surg 2005;34(4), 411-419.
  • 3. Jiang X, Zhang Y, Fan X, Deng X, Zhu Y, Li F. The effects of hypoxia-inducible factor (HIF)-1α protein on bone regeneration during distraction osteogenesis: an animal study. Int J Oral Maxillofac Surg 2016;45(2):267-272.
  • 4. Freddo AL, Giongo CC, Ponzoni D, Corsetti A, Puricelli E. Influence of a Magnetic Field and Laser Therapy on the Quality of Mandibular Bone During Distraction Osteogenesis in Rabbits. J Oral Maxillofac Surg 2016;74(11):2287.e1-2287.e8. 5. Williams PR, Smith NC, Cooke-Yarborough C, Little DG. Bisphosphonates and nephrocalcinosis in a rabbit leg lengthening model: a histological and therapeutic comparison. Pharmacol Toxicol 2001;89:149-152.
  • 6. Primrose AC, Broadfoot E, Diner PA, Molina F, Moos KF, Ayoub AF. Patients' responses to distraction osteogenesis: a multi-centre study. Int J Oral Maxillofac Surg 2005;34(3):238-242.
  • 7. Zhu S, Song D, Jiang X, Zhou H, Hu J. Combined effects of recombinant human BMP-2 and Nell-1 on bone regeneration in rapid distraction osteogenesis of rabbit tibia. Injury 2011;42(12):1467-1473.
  • 8. Yonezawa H, Harada K, Ikebe T, Shinohara M, Enomoto S. Effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) on bone consolidation on distraction osteogenesis: a preliminary study in rabbit mandibles. J Craniomaxillofac Surg 2006;34(5):270-276.
  • 9. Castro-Govea Y, Cervantes-Kardasch VH, Borrego-Soto G, Martínez-Rodríguez HG, Espinoza-Juarez M, Romero-Díaz V, Marino-Martínez IA, Robles-Zamora A, Álvarez-Lozano E, Padilla-Rivas GR, Ortiz-López R, Lara-Arias J, Vázquez-Juárez J, Rojas-Martínez A. Human bone morphogenetic protein 2-transduced mesenchymal stem cells improve bone regeneration in a model of mandible distraction surgery. J Craniofac Surg 2012;23(2):392-396.
  • 10. Alp YE, Taskaldiran A, Onder ME, Karahan S, Kocyigit ID, Atil F, Tekin U. Effects of Local Low-Dose Alendronate Injections Into the Distraction Gap on New Bone Formation and Distraction Rate on Distraction Osteogenesis. J Craniofac Surg 2017;28(8):2174-2178.
  • 11. Taylor BA, Bezuhly M, Brace M, Carter M, Hong P. Effect of strontium citrate on bone consolidation during mandibular distraction osteogenesis. Laryngoscope 2017;127(7):e212-e218.
  • 12. Onger ME, Bereket C, Sener I, Ozkan N, Senel E, Polat AV. Is it possible to change of the duration of consolidation period in the distraction osteogenesis with the repetition of extracorporeal shock waves? Med Oral Patol Oral Cir Bucal 2017;22(2):e251-e257.
  • 13. Martini L, Giavaresi G, Fini M, Torricelli P, de Pretto M, Schaden W, Giardino R. Effect of extracorporeal shock wave therapy on osteoblastlike cells. Clin Orthop Relat Res 2003;413:269-280.
  • 14. Huang HM, Li XL, Tu SQ, Chen XF, Lu CC, Jiang LH. Effects of roughly focused extracorporeal shock waves therapy on the expressions of bone morphogenetic protein-2 and osteoprotegerin in osteoporotic fracture in rats. Chin Med J (Engl) 2016;129(21):2567-2575.
  • 15. Wang FS, Yang KD, Kuo YR, Wang CJ, Sheen-Chen SM, Huang HC, Chen YJ. Temporal and spatial expression of bone morphogenetic proteins in extracorporeal shock wave-promoted healing of segmental defect. Bone 2003;32:387-396.
  • 16. Wang CJ, Wang FS, Yang KD. Biological Effects of Extracorporeal Shockwave in Bone Healing: A Study in Rabbits, Arch Orthop Trauma Surg 2008;128:879-884.
  • 17. Lai JP, Wang FS, Hung CM, Wang CJ, Huang CJ, Kuo YR. Extracorporeal shock wave accelerates consolidation in distraction osteogenesis of the rat mandible. J Trauma 2010;69(5):1252-1258.
  • 18. Şahin B, Emirzeoglu M, Uzun A, İncesu L, Bek Y, Bilgiç S, Kaplan S. Unbiased estimation of the liver volume by the Cavalieri principle using magnetic resonance images. Eur J Radiol 2003;47:164–170.
  • 19. Bulut O, Eroglu M, Ozturk H, Tezeren G, Bulut S, Koptagel E. Extracorporeal shock wave treatment for defective nonunion of the radius: a rabbit model. J Orthop Surg 2006;14(2):133-137.
  • 20. Potres Z, Deshpande S, Klöeppel H, Voss K, Klineberg I. Assisted wound healing and vertical bone regeneration with simultaneous implant placement: a histologic pilot study. Int J Oral Maxillofac Implants 2016;31(1):45-54.
  • 21. van der Jagt OP, Piscaer TM, Schaden W, Li J, Kops N, Jahr H, van der Linden JC, Waarsing JH, Verhaar JA, de Jong M, Weinans H. Unfocused extracorporeal shock waves induce anabolic effects in rat bone. J Bone Joint Surg Am 2011;93(1):38-48.
  • 22. Kearney CJ, Hsu HP, Spector M. The use of extracorporeal shock wave-stimulated periosteal cells for orthotopic bone generation. Tissue Eng Part A 2012;18(13-14):1500-1508.
  • 23. Ma D, Ren L, Yao H, Tian W, Chen F, Zhang J, Liu Y, Mao T. Locally injection of cell sheet fragments enhances new bone formation in mandibular distraction osteogenesis: a rabbit model. J Orthop Res 2013;31(7):1082-1088.
  • 24. van der Jagt OP, van der Linden JC, Schaden W, van Schie HT, Piscaer TM, Verhaar JA, Weinans H, Waarsing JH. Unfocused extracorporeal shock wave therapy as potential treatment for osteoporosis. J Orthop Res 2009;27(11):1528-1533.
  • 25. Çakir-Özkan N, Bereket C, Arici N, Elmali M, Şener I, Bekar E. The radiological and stereological analysis of the effect of low-level laser therapy on the mandibular midline distraction osteogenesis. J Craniofac Surg 2015;26(7):e595-599.
  • 26. Wang FS, Yang KD, Wang CJ, Huang HC, Chio CC, Hsu TY, Ou CY. Shockwave stimulates oxygen radical-mediated osteogenesis of the mesenchymal cells from human umbilical cord blood. J Bone Miner Res 2004;19(6):973-982.
  • 27. Özkan E, Bereket MC, Önger ME, Polat AV. The Effect of Unfocused Extracorporeal Shock Wave Therapy on Bone Defect Healing in Diabetics. J Craniofac Surg 2018;29(4):1081-1086.
  • 28. Ding Y, Li G, Ao J, Zhou L, Ma Q, Liu Y. 99mTechnetium-methylene diphosphonate bone imaging using low-intensity pulsed ultrasound: promotion of bone formation during mandibular distraction osteogenesis in dogs. Br J Oral Maxillofac Surg 2010;48:94-99.
  • 29. Cerqueira A, Silveira RL, Oliveira MG, Sant'ana Filho M, Heitz C. Bone tissue microscopic findings related to the use of diode laser (830 nm) in ovine mandible submitted to distraction osteogenesis. Acta Cir Bras 2007;22(2):92-97.
  • 30. Freddo AL, Hübler R, de Castro-Beck CA, Heitz C, de Oliveira MG. A preliminary study of hardness and modulus of elasticity in sheep mandibles submitted to distraction osteogenesis and low-level laser therapy. Med Oral Patol Oral Cir Bucal 2012;17(1):e102-107.
Year 2019, Volume: 53 Issue: 3, 125 - 131, 01.10.2019

Abstract

Project Number

PYO.DIS.1904.12.007

References

  • 1. Raza H, Saltaji H, Kaur H, Flores-Mir C, El-Bialy T. Effect of Low-Intensity Pulsed Ultrasound on Distraction Osteogenesis Treatment Time: A Meta-analysis of Randomized Clinical Trials. J Ultrasound Med 2016;35(2):349-358.
  • 2. Zimmermann C, Thurmuller M, Troulis D, Perrott B, Rahn L, Kaban B. Histology of the porcine mandibular distraction wound. Int J Oral Maxillofac Surg 2005;34(4), 411-419.
  • 3. Jiang X, Zhang Y, Fan X, Deng X, Zhu Y, Li F. The effects of hypoxia-inducible factor (HIF)-1α protein on bone regeneration during distraction osteogenesis: an animal study. Int J Oral Maxillofac Surg 2016;45(2):267-272.
  • 4. Freddo AL, Giongo CC, Ponzoni D, Corsetti A, Puricelli E. Influence of a Magnetic Field and Laser Therapy on the Quality of Mandibular Bone During Distraction Osteogenesis in Rabbits. J Oral Maxillofac Surg 2016;74(11):2287.e1-2287.e8. 5. Williams PR, Smith NC, Cooke-Yarborough C, Little DG. Bisphosphonates and nephrocalcinosis in a rabbit leg lengthening model: a histological and therapeutic comparison. Pharmacol Toxicol 2001;89:149-152.
  • 6. Primrose AC, Broadfoot E, Diner PA, Molina F, Moos KF, Ayoub AF. Patients' responses to distraction osteogenesis: a multi-centre study. Int J Oral Maxillofac Surg 2005;34(3):238-242.
  • 7. Zhu S, Song D, Jiang X, Zhou H, Hu J. Combined effects of recombinant human BMP-2 and Nell-1 on bone regeneration in rapid distraction osteogenesis of rabbit tibia. Injury 2011;42(12):1467-1473.
  • 8. Yonezawa H, Harada K, Ikebe T, Shinohara M, Enomoto S. Effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) on bone consolidation on distraction osteogenesis: a preliminary study in rabbit mandibles. J Craniomaxillofac Surg 2006;34(5):270-276.
  • 9. Castro-Govea Y, Cervantes-Kardasch VH, Borrego-Soto G, Martínez-Rodríguez HG, Espinoza-Juarez M, Romero-Díaz V, Marino-Martínez IA, Robles-Zamora A, Álvarez-Lozano E, Padilla-Rivas GR, Ortiz-López R, Lara-Arias J, Vázquez-Juárez J, Rojas-Martínez A. Human bone morphogenetic protein 2-transduced mesenchymal stem cells improve bone regeneration in a model of mandible distraction surgery. J Craniofac Surg 2012;23(2):392-396.
  • 10. Alp YE, Taskaldiran A, Onder ME, Karahan S, Kocyigit ID, Atil F, Tekin U. Effects of Local Low-Dose Alendronate Injections Into the Distraction Gap on New Bone Formation and Distraction Rate on Distraction Osteogenesis. J Craniofac Surg 2017;28(8):2174-2178.
  • 11. Taylor BA, Bezuhly M, Brace M, Carter M, Hong P. Effect of strontium citrate on bone consolidation during mandibular distraction osteogenesis. Laryngoscope 2017;127(7):e212-e218.
  • 12. Onger ME, Bereket C, Sener I, Ozkan N, Senel E, Polat AV. Is it possible to change of the duration of consolidation period in the distraction osteogenesis with the repetition of extracorporeal shock waves? Med Oral Patol Oral Cir Bucal 2017;22(2):e251-e257.
  • 13. Martini L, Giavaresi G, Fini M, Torricelli P, de Pretto M, Schaden W, Giardino R. Effect of extracorporeal shock wave therapy on osteoblastlike cells. Clin Orthop Relat Res 2003;413:269-280.
  • 14. Huang HM, Li XL, Tu SQ, Chen XF, Lu CC, Jiang LH. Effects of roughly focused extracorporeal shock waves therapy on the expressions of bone morphogenetic protein-2 and osteoprotegerin in osteoporotic fracture in rats. Chin Med J (Engl) 2016;129(21):2567-2575.
  • 15. Wang FS, Yang KD, Kuo YR, Wang CJ, Sheen-Chen SM, Huang HC, Chen YJ. Temporal and spatial expression of bone morphogenetic proteins in extracorporeal shock wave-promoted healing of segmental defect. Bone 2003;32:387-396.
  • 16. Wang CJ, Wang FS, Yang KD. Biological Effects of Extracorporeal Shockwave in Bone Healing: A Study in Rabbits, Arch Orthop Trauma Surg 2008;128:879-884.
  • 17. Lai JP, Wang FS, Hung CM, Wang CJ, Huang CJ, Kuo YR. Extracorporeal shock wave accelerates consolidation in distraction osteogenesis of the rat mandible. J Trauma 2010;69(5):1252-1258.
  • 18. Şahin B, Emirzeoglu M, Uzun A, İncesu L, Bek Y, Bilgiç S, Kaplan S. Unbiased estimation of the liver volume by the Cavalieri principle using magnetic resonance images. Eur J Radiol 2003;47:164–170.
  • 19. Bulut O, Eroglu M, Ozturk H, Tezeren G, Bulut S, Koptagel E. Extracorporeal shock wave treatment for defective nonunion of the radius: a rabbit model. J Orthop Surg 2006;14(2):133-137.
  • 20. Potres Z, Deshpande S, Klöeppel H, Voss K, Klineberg I. Assisted wound healing and vertical bone regeneration with simultaneous implant placement: a histologic pilot study. Int J Oral Maxillofac Implants 2016;31(1):45-54.
  • 21. van der Jagt OP, Piscaer TM, Schaden W, Li J, Kops N, Jahr H, van der Linden JC, Waarsing JH, Verhaar JA, de Jong M, Weinans H. Unfocused extracorporeal shock waves induce anabolic effects in rat bone. J Bone Joint Surg Am 2011;93(1):38-48.
  • 22. Kearney CJ, Hsu HP, Spector M. The use of extracorporeal shock wave-stimulated periosteal cells for orthotopic bone generation. Tissue Eng Part A 2012;18(13-14):1500-1508.
  • 23. Ma D, Ren L, Yao H, Tian W, Chen F, Zhang J, Liu Y, Mao T. Locally injection of cell sheet fragments enhances new bone formation in mandibular distraction osteogenesis: a rabbit model. J Orthop Res 2013;31(7):1082-1088.
  • 24. van der Jagt OP, van der Linden JC, Schaden W, van Schie HT, Piscaer TM, Verhaar JA, Weinans H, Waarsing JH. Unfocused extracorporeal shock wave therapy as potential treatment for osteoporosis. J Orthop Res 2009;27(11):1528-1533.
  • 25. Çakir-Özkan N, Bereket C, Arici N, Elmali M, Şener I, Bekar E. The radiological and stereological analysis of the effect of low-level laser therapy on the mandibular midline distraction osteogenesis. J Craniofac Surg 2015;26(7):e595-599.
  • 26. Wang FS, Yang KD, Wang CJ, Huang HC, Chio CC, Hsu TY, Ou CY. Shockwave stimulates oxygen radical-mediated osteogenesis of the mesenchymal cells from human umbilical cord blood. J Bone Miner Res 2004;19(6):973-982.
  • 27. Özkan E, Bereket MC, Önger ME, Polat AV. The Effect of Unfocused Extracorporeal Shock Wave Therapy on Bone Defect Healing in Diabetics. J Craniofac Surg 2018;29(4):1081-1086.
  • 28. Ding Y, Li G, Ao J, Zhou L, Ma Q, Liu Y. 99mTechnetium-methylene diphosphonate bone imaging using low-intensity pulsed ultrasound: promotion of bone formation during mandibular distraction osteogenesis in dogs. Br J Oral Maxillofac Surg 2010;48:94-99.
  • 29. Cerqueira A, Silveira RL, Oliveira MG, Sant'ana Filho M, Heitz C. Bone tissue microscopic findings related to the use of diode laser (830 nm) in ovine mandible submitted to distraction osteogenesis. Acta Cir Bras 2007;22(2):92-97.
  • 30. Freddo AL, Hübler R, de Castro-Beck CA, Heitz C, de Oliveira MG. A preliminary study of hardness and modulus of elasticity in sheep mandibles submitted to distraction osteogenesis and low-level laser therapy. Med Oral Patol Oral Cir Bucal 2012;17(1):e102-107.
There are 29 citations in total.

Details

Primary Language English
Subjects Dentistry, Health Care Administration
Journal Section Original Research Articles
Authors

Erman Senel This is me

Enes Ozkan This is me

Mehmet Cihan Bereket This is me

Mehmet Emin Onger This is me

Project Number PYO.DIS.1904.12.007
Publication Date October 1, 2019
Submission Date September 20, 2018
Published in Issue Year 2019 Volume: 53 Issue: 3

Cite

EndNote Senel E, Ozkan E, Bereket MC, Onger ME (October 1, 2019) The assessment of new bone formation induced by unfocused extracorporeal shock wave therapy applied on pre-surgical phase of distraction osteogenesis. European Oral Research 53 3 125–131.