Lignocellulosic Ezymes of Basidial Fungi-Isolated from Different Ecological Niches of Georgia

Abstract

Great interest in basidiomycetes for targeted technological treatment of agro-industrial plant substrates are conditioned by their ability to produce lignocellulosic enzymes. Consisting of 29 soil-climatic zones, Georgia (South Caucasus area) represents a unique place for the isolation of microorganisms. In order to study the biochemistry and physiology of wood-degrading basidiomycetes, samples of wood-degrading basidiomycetes were collected from different taxonomic niches of Georgia. 45 strains were obtained as pure cultures and 32 ones – identified. Producers of lignocellulosic enzymes were revealed among test fungi under solid-state and submerged cultivation conditions. Pleurous ostreatus GV10, Pleurotus drynus IN 11 and Fomes fomentarius GK33 were found to be the best producers of cellulosic enzymes using orange and mandarin peels, wheat and Potato straw, wheat bran as substrates, and Ganoderma lucidum GB03 – the best producer of laccase during cultivation on orange and mandarin peels waste. The influence of lignocellulose on the accumulation of the enzymes laccase, xylanase and Filter paper assay was studied.

Keywords

• lignocellulosic materials
• Basidiomycetes
• laccase
• cellulase
• xylanase.

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