Inhibition of the Growth of Staphylococcus Aureus by Using Some Antibiotics Produced from Bacillus Subtilis

Abstract

Bacillus subtilis can produce many substances that are considered as a means of defense in addition to being tested and approved as antibiotics. The goal of this study was to see if Bacillus subtilis crude bacteriocin had any antibacterial effects on Staphylococcus aureus .The peptidic bacteriocin is considered of importance in the industrial and medical fields as an anticancer.We got our bacteria from the Bacterial Bank at the College of Science and the Department of Biology. Identification of the isolates was confirmed by microscopically and biochemical tests which include catalase, oxidase, IMViC, blood hemolysis, starch hydrolysis for Bacillus, and growth on Mannitol Salt Agar (MSA), Voges-Jenson agar(V.J), and Coagulase test for Staphylococcus. Based on the capacity of bacteriocin to diffuse in agar, the reverse technique, which eliminated the contact between the producers and sensitive strains, was used to detect bacteriocin antibacterial action. Results demonstrate the antibacterial effect of Bacillus subtilis bacteriocin by the inhibition of Staphylococcus aureus as compared to other indicator isolates.

Keywords

• Bacillus.subtilis
• Staphylococcus.aureus
• Bacteriocin

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