Synthesis and Characterization of Gold/Silver Bimetallic Nanoparticles Using Trastuzumab: An Enhanced Anti-Cancer Activity

Abstract

Bimetallic nanoparticles are getting a lot of attention in medicine and society because they might have interesting physical and synthetic properties, such as higher affinity, a lower molecular weight, and a larger surface area. Trastuzumab capped gold/silver bimetallic nanoparticles (Au/Ag-BNPs) were prepared for the purpose of enhancing the effects of Au/Ag-BNPs on SKBR3 breast cancer cells in order to reduce trastuzumab resistance. Au/Ag-BNPs were produced chemically. These Au/Ag-BNPs were characterized using various techniques. UV-Visible (UV-Vis) spectroscopy were used to monitor Plasmon absorption maxima at 462 nm. X-ray powder diffraction (XRD) pattern was utilized to confirm the crystalline nature of the BNPs. Lastly, Fourier transformed infrared (FT-IR) spectroscopy was used to investigate the bonding patterns of the NPs. In SKBR3 human breast cancer cells, the biological characteristics of these NPs were exemplary. Based on the results, we concluded that these gold/silver bimetallic nanoparticles have anticancer potential and can serve as an alternative to decrease the trastuzumab resistance of SKBR3 breast cancer cells. However, additional research is required to corroborate these results.

Keywords

• Bimetallic nanoparticles
• Trastuzumab resistance
• SKBR3 cells
• Apoptosis

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