Antagonistic of some Trichoderma against Fusarium Oxysporum sp. f. cubense Tropical Race 4 (FocTR4)

Abstract

Fusarium wilt of banana is a very important fungus that caused the destruction of banana trees in the tropical countries. Biological control is an alternative method to control fusarium wilt diseases such as Trichoderma has been known to be particularly active in the control of the plant pathogens. This study aimed to evaluate the ability Trichoderma isolates from suppressive soils in Malaysia to suppress Fusarium wilt of banana in vitro. Thirty one of Trichoderma isolates were tested their ability to inhibit the growth of FocTR4 LJ27 strain. The isolates were screened in vitro by volatile compounds tested of Trichoderma isolates against LJ27 strain. Then eight Trichoderma spp. strains (TR10, T10v1, T1, Tveg2, TR102, TL5, Tveg1, T26) was produced the high toxic metabolites with strong activity against LJ27 strain, inhibiting the mycelia growth by 50.33%, 51.33%, 51.67%, 69%, 70.67%, 71.33%, 78%, 96% respectively. The result indicated to a high efficacy of T. parareesei T26 for inhibiting the growth of FocTR4. But five isolates of Trichoderma such as T. brevicompactum (TL7), T. reesei (T658, TL102, and TL13552), and T. harzianum (TL21) were showing the very low effect on FocTR4. The volatile compounds can produce for the inhibiting of developing of FocTR4 in vitro. This improves the high efficacy of Trichoderma to use as alternative methods in reducing the synthetic chemicals that are causing a toxic pollution for our environment. 

Keywords

• FocTR4,Trichoderma,Biocontrol,Banana,Fusarium wilt

References

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