Determination of Morphology and Allergenic Proteins of Pistachio (Pistachia vera L.) Pollens in Gaziantep

Abstract

Allergens can be defined as antigens that stimulate specific IgE (Immunoglobulin E) antibody formation and react with it. In this study, the morphologies of Pistachia vera allergenic pollens belonging to Anacardiaceae family in South Eastern Anatolia were examined by light microscopy and pollens extracts were prepared from these species. The specimens used in morphological studies were obtained from Gaziantep University campus area. Slides of pollens were prepared according to Wodehouse (1935) method and the morphological characteristics of pollens were determined using microphotographs taken by light microscopy. Pollens crude extracts were obtaıned from the flora grown in this region. Extraction method established by Aytug et al. (1991) was used to prepare crude extracts from the collected pollens, 'Coca' solution was added extensively and sterile filtration technique was used for sterilization. BCA assay and absorbance measurements were used to determine the protein concentration. Standard curve was generated using concentrations and absorbance values of standard protein samples prepared at different concentrations (0, 1-1mg/ml). The protein concentration of the pollens extracts was calculated using the correct equation obtained from this curve. These crude extracts contain many major and minor allergens that can be used to diagnose allergic diseases. Due to the very high concentration of allergenic proteins in P. vera, it can be included in allergen kits. Moreover, allergy tests should be conducted to those living in this region. The results obtained from this study can both contribute to systematic studies and to the treatment of allergic diseases caused by these types of pollens.

Keywords

• Pollen allergy,Pollen extract,P. vera,Pollen morphology

References

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