Determination of Potential Allergenic Proteins and Morphologenic Proteins and Morphology of Linden (Tilia cordata), Anatolian oak (Quercus ithaburensis) and Birch (Betula alba) Pollens in Gaziantep

Abstract

Allergic diseases are a major public health problem in the modern societies. Pollens dispersed through wind are one of the major aeroallergens. When they are released to atmosphere in sufficient amount, they can cause the development of diseases such as asthma, rhinitis, rhinoconjunctivitis etc. in allergically hypersensitive individuals. The spreading of pollens are influenced by their amount in the air, their structures, geographic areas and the climate. Therefore, the types of pollens that hypersensitive individuals who live in different regions are exposed to may differ and different allergic reactions may occur in affected individuals. The high amount of allergens in pollens enhance the sensitivity to pollens. Thus the research in region-specific plant species allergenic effects is very important. Pollens of linden (Tilia cordata), Anatolian oak (Quercus ithaburensis) and birch (Betula alba) are important allergen sources in Gaziantep province. Pollen allergens are water soluble, stable proteins or glycoproteins of molecular weight between 5-80 kD. A single pollen type usually contains several different allergens. Pollens from linden (T. cordata), Anatolian oak (Q. ithaburensis) and birch (B. alba) were collected during pollination period and their extracts were prepared. For identification of pollens morphology slides were prepared according to Wodehouse’s method and images were taken under light microscope. Total concentrations of potential allergen proteins were determined from prepared pollen extracts using the BCA method. In this study, we aimed to prepare extracts of pollens from linden, Anatolian oak and birch widely grown in wooded areas of Gaziantep University for the study of allergens and their use for diagnosing allergic diseases.

Keywords

• Allergic diseases,Tilia cordata,Quercus ithaburensis,Betula alba,pollen extract

References

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