Cell Isolating from Bovine Pericardial Fluid and Culturing for Next Tissue Engineering Applications

Abstract

Hearth is an important organ that is a responsible for pumping blood whole body, and so cardiac diseases are critically important all over the world. Pericardial fluid (PF) and its component are important for diagnosis and treatment for heart disease. In literature, PF has been analysed especially for pericarditis disease, and there has been any research on PF for tissue engineering application. The aim of this study is isolating the cells from the pericardial fluid and culturing these cells in vitro in order to be used as cell line in tissue engineering studies. PF contains heterogen cell population which are lymphocytes, glanulocytes, macrophages, eosinophils and basophils, These cells were cultured in five different media (Alpha MEM, DMEM-Low, EMEM, MCDB 131, and Med 199) in this study. As a result of cell culture was performed and concluded that, cell proliferation and viability were the best in culture with Alpha MEM media according to the microscope examination. 

Keywords

• Pericardial fluid,Heart,Cell culturing,Tissue engineering

References

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