Preparation of Gelatin and Chitosan Hydrogels Beads

Abstract

In this study, a series of gelatin and chitosan hydrogel beads were prepared via covalently crosslinking of these polymers. At the first step, three types of gelatin beads were obtained by dropping polymer solution into Glutaraldehyde (GA)-oil emulsion. While the concentration of polymer solution was varied from 7% to 14%, the concentration of crosslinking solution kept constant. Secondly, two types of chitosan beads were prepared by ionic gelation interaction procedure using Sodium Tripolyphosphate (TPP) as crosslinker. 1% of polymer solution was dropped into TPP solutions at different concentrations. At the last step, the different series of gelatin hydrogels were prepared by cutting the crosslinked polymer roads to form beads.  Swelling behaviors of all the hydrogel beads were studied by changing time at constant temperature and pH. Swelling percentages (S%) increased with time initially, and then remained constant at close to 24 h. In general, it was determined that the gelatin hydrogel beads were much more swellable than the chitosan beads.    

Keywords

• Gelatin,Chitosan,Hydrogel,Glutaraldehyde

References

1. Bigi A., Cojazzi G., Panzavolta S., Rubini K., Roveri N. (2001). Mechanical and ther¬mal properties of gelatin films at different degrees of glutaraldehyde crosslinking. Biomat. 22, 763-768. Blanco M.D., Bernardo M.V., Teijon C., Sastre R.L., Teijon J.M.: Transdermal application of bupivacaine-loaded p (AAm-co-mm itacon) hydrogels. Int. J. Pharm. 255,99-107 (2003). Chen K., Ku Y., Lin H., Yan T., Sheu D., Chen T., Lin F. (2005). Preparation and char¬acterization of pH-sensitive poly (N-vinyl 2-pyrrolidone/itaconic acid) copolymer hydrogels. Mat. Chem. Phys. 91, 484-489. El-Sherbiny I.M., Lins R.J., Abdel-Bary E.M., Harding D.R.K.(2005). Preparation, char¬acterization, swelling and in vitro drug release behaviour of poly(N-AG-chitosan) interpolymeric pH and therm.-responsive hydrogels. Europ. Polym. J. 41, 2584-2591. Gilsenan P, Ross-Murphy S. 2001. Shear creep of gelatin gels from mammalian and piscine collagens. International Journal of Biological Macromolecules 29: 53–61. Kotze´A F, et all. (1999). Chitosan for enhanced intestinal permeability: prospects for derivatives soluble in neutral and basic environments. Eur J Pharma Sci. 7:2, 145-151. Kushibiki T, Tomoshige R, Fukunaka Y, Kakemi M, Tabataa Y. (2003). In vivo release and gene expression of plasmid DNA by hydrogels of gelatin with different cationization extents. Journal of Control Release 90: 207–216. Macleod GS, et.all.(1999). Selective drug delivery to the colon using pectin, chitosan, hydroxypropyl methylcellulose film coated tablets. Int J Pharm. 187:251–257. Nitsae M., Madjid A., Hakim L. and Sabarudin A., (2016). Preparation of chitosan beads using trıpolyphosphate and ethylene glycol diglycıdyl ether as crosslinker for Cr(vı) adsorption. Chemistry & chemical Technology, Vol. 10, No. 1., 105-113. Pulat M, Tan N, Onurdag FK. (2011). Swelling Dynamics of IPN Hydrogels Including Acrylamide-Acrylic Acid-Chitosan and Evaluation of their Potential for Controlled Release of Piperacillin-Tazobactam, Journal of Applied Polymer Science, Vol. 120, 441–450. Pulat M., Asıl D. (2009). Fluconazole release through semi-ipn hydrogels based on chitosan, AA, and citraconic acid. J. Appl. Polym. Sci. 113, 2613-2619. Pulat M., Yoltay N. (2016). Smart fertilizers: preparation and characterization of gelatin-based hydrogels for controlled release of MAP and AN fertilizers, Agrochimica, 60:4, 249-261. Pulat M., Akalın G.O. (2013). Preparation and characterization of gelatin hydrogel support for immobilization of C. Rugosa lipase. Artif. Cells Nanomed. Biotechnol. 41, 145-151. Sapana P Ahirrao, Paraag S Gide, B Shrivastav, and Pankaj Sharma.Ionotropic Gelation: (2014).A Promising Cross Linking Technique for Hydrogels. research and revıews: journal of pharmaceutıcs and nanotechnology, 2 :1, 1-6.