Evaluation of Pistacia vera Sap Waste Sections and Its Potential Role on Treatment

Abstract

It is estimated that 1.3 billion waste is produced annually in the world and this amount is expected to increase to 38-67% by the end of 2025. Considering the waste production of pistachio, it is seen that around 132 165 thousand tons of waste products have been accumulated after the product processing phase due to excessive consumption and wide usage area. However, it has been reported that there are important secondary metabolites such as "masticadienonic acid", "masticadienolic acid", "tirucallol" and "pistasionic acid" in the extract content of unused waste stem parts. Masticadienonic acid has been shown in the literature to have anti-proliferative and apoptotic activity. It has been determined that masticadienolic acid component has cytotoxic activity on five different cancer cell lines (Leukemia, Breast, Prostate, Colon and CNS). Examining the studies on the Tirukallol component, it was determined that it inhibits adhesion molecules in human endothelial cells. Pistacionic acid is a newly purified and characterized compound and only anticholinesterase and antidiabetic activities have been studied. The aim of our study is to determine whether these components obtained from stem parts have any cytotoxic effect on Mcf7 cells. Within the scope of the study, the stems were obtained from pistachio processing plants and kept at room temperature, in a cool environment. In order to obtain the compounds from sap extracts, column chromatography, ion layer chromatography (TLC) and crystallization methods which are moving phase systems were used. Mcf7 cells were exposed to different concentrations of the purified components and their cytotoxic activity was evaluated using the MTT test. Mcf7 cells were exposed to different concentrations of the purified components and their cytotoxic activity was evaluated using the MTT test. The results of our study are important preliminary data in the literature and we believe that they may contribute to further studies.

Keywords

• Waste Solid
• Pistacia vera
• Bioactive Metabolite
• Antitumoral activity
• Phytotherapy

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