Allergenic Proteins of Tilia Cordata

Abstract

There are a lot of environmental allergenic factors; pollens, fungal spores, house dust mites, some fooods and drinks. These factors are harmless for many people in despite of making hypersensitivity any people. This sensitivity is calling “allergy’’. Allergens generally has a protein structure and when Charles Blakey’s discovered of pollens making allergenic diseases in 1873 after that pollens have determined a pivotal role to allergenic diseases. The pollens allergenic qualifications have known orginated layers of exine and intine including of glycoproteins, lipoproteins, polysaccaride and proteins. In this study, we investigated that allergenic proteins of Tilia cordata collected from Gaziantep. T. cordata pollen samples were collected during dissemination period and pollen extract prepared. We used Wodehouse method and prepared slides for determination of pollen morfology and were taken photos under light microscopy and measured morphological parameters. Total concentration of pollen proteins was determined using by BCA method. The amount of T.cordata allergen proteins was calculated as standard graph obtained from BSA. The average diameter of the T. cordata pollens diameter was measured as 88,83 μm and allergenic proteins from T. cordata was determined as significantly high. 1259.28 μg/ml concentration of T. cordata protein was measured. We suggested that this study provides important data to the literature by determining the amount of allergenic protein of T. cordata in this region because of differences about the content of allergenic proteins among regions.

Keywords

• Allergy
• Allergen
• Pollen
• T. cordata

References

1. Behrendt, H. & Becker, W.M., (2001). Localization, release and bioaviability of pollen allergens: the influence of enviromental factors, Journal Current Opinion in Immunology, 13,709-715
2. Boral, D., Chatterjee, S. & Bhattacharya, K. (2004). The occurrence and allergengising potential of airborne pollen in West Bengal. India, Annals of Agricultural and Enviromental Medicine, 11, 45-52.
3. Celik, A., Guvensen, A., Uysal, I. & Ozturk, M., (2005), Differences in concentrations of allergenic pollens at different heights in Denizli, Turkey, Pakistan Journal of Botany, 37(3), 519-530.
4. Dykewicz, M.S., & Hamilios, D.L. (2010). Rhinitis and sinusitis, Journal of Allergy and Clinical Immunology, 125, 103-15
5. Heptt, W., Dinh, Q.T., Cryer, A., Zweng, M., Noga, O., Peiser, C.,Melvan, M., Witt, C., Fincher, A. & Gronberg, D.A. (2004). Phenotypic alteration of neuropeptide-containing nervefibres in seasonal intermittent allergic rhinitis, Clinical and Experimental Allergy, 34, 1105-10.
6. Knox, R.B. (1979), Pollen and allergy, London, Edward arnold limited, 60p.
7. Knox R.B. (1984). The pollen grain. Embryology of angiosperms. Springer, Berlin,pp. 197-271
8. Ring, J., Przybilla, B., & Ruzicka, T., (2006). Atopy: Condition, Disease, or Syndrome?, Handbook of Atopic Eczama, 2th ed. Springer, Germany, ISBN 3-540-23133-1

9. Rauder, C & Breiteneder, H. (2006), Pollen allergens are restricted to few proteins familles and show distinct patterns of species distribution, Journal of Allergy and Clinical Immunology, 117, 141-7.
10. Spieksma, F.T.M., (1991). Regional Europen pollen calenders. In: D’Amato G, Spieksma, F.T.M, Bonini S Editors, Allergenic pollen and polinosis in Europe. Oxford: Blackwell Sci. Publ., 49-65
11. Weerd, N.A., Bhalla, P.L & Singh, M.B., (2002). Aeroallergens and polinosis: molecular and immunological characteristics of cloned pollen allergens, Aerobiologia 18, 87-106