Çeşitli Hayvansal Klinik Örneklerden İzole Edilen Staphylococcus aureus Suşlarında Slaym Pozitifliği ve Antibiyotik Direnci

Year 2014, Volume: 11 Issue: 3, 163 - 168, 01.12.2014

Esra Şeker Nilgün Ünal

Abstract

Bu çalışmada, inek sütü, inek uterus sıvabı, köpek kulak sıvabı, köpek deri sıvabı ve tavuk sinoviyal sıvı örneklerinden

izole edilen toplam 50 Staphylococcus aureus suşu slaym faktör üretimi ve antibiyotik direnci yönünden incelendi.

Suşların slaym üretimi Congo red agar ve standart tüp yöntemleri kullanılarak belirlendi. Elli S. aureus suşunun 21’i

(%42) Congo red agarda, 18’i (%36) standart tüp yöntemi ile slaym pozitif olarak belirlendi. İki farklı yöntem arasında

istatistiksel olarak önemli bir farklılık bulunmadı (p>0.05). İzolatların antibiyotik dirençlerinin belirlenmesinde standart Etest

yöntemi kullanıldı. İzolatlar arasında en yüksek direnç oranı penisilin G’ye (%20) karşı iken, bunu sefalotin (%16),

oksasilin (%16) ve tetrasikline (%14) karşı direnç oranları izledi. Test edilen izolatlardan sadece biri (%2) enrofloksasine

karşı dirençliydi. Eritromisin, trimetoprim/sulfametoksazol, rifampin ve gentamisine karşı ise direnç tespit edilemedi.

Slaym pozitif ve negatif suşların antibiyotik dirençleri karşılaştırıldığında, slaym pozitif suşlarda penisilin G, sefalotin ve

oksasiline karşı direnç önemli oranda yüksekti (p<0.05).

Keywords

Antibiyotik direnci, slaym faktör, Staphylococcus aureus

Slime Positivity and Antibiotic Resistance in Staphylococcus aureus Strains Isolated from Various Animal Clinical Samples

Abstract

In this study, a total of 50 Staphylococcus aureus strains isolated from bovine milk, bovine uterus swabs,

dog ear swab, dog skin swab and chicken synovial fluid samples were examined in terms of slime factor production

and antibiotic resistance. The slime production of strains was determined by using Congo red agar and standard tube

methods. Of 50 S. aureus strains, 21 (42%) and 18 (36%) were determined as slime positive in the Congo red agar

and standard tube methods, respectively. A significant difference was not found between two different methods

(p>0.05). Standard E-test method was used to detect the antibiotic resistance of isolates. Among the isolates, the

highest resistance rate was against penicillin G (20%), followed by cephalothin (16%), oxacillin (16%) and tetracycline

(14%). Only one (2%) of the tested strains was resistant to enrofloxacin. However, resistance to erythromycin,

trimethoprim/sulfamethoxazole, rifampin and gentamicin were not determined in any strains. When the antibiotic

resistance of slime positive and negative strains was compared, resistance to penicillin G, cephalothin and oxacillin

was significantly high in slime positive strains (p<0.05).

Keywords

Antibiotic resistance, slime factor, Staphylococcus aureus

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