Effects of Non-Surgical Periodontal Treatment on Gingival Crevicular Fluid of Interleukin 26 Levels at Stage III Grade C Periodontitis Patients

Abstract

Objectives The aim of this study was to determine interleukin (IL) 26 and IL-6 levels of gingival crevicular fluid (GCF) in stage III grade C periodontitis (SIII-C-P) and healthy individuals, and to evaluate the effect of non-surgical periodontal therapy (NSPT) on IL-26 and IL-6 levels in SIII-C-P patients. Materials and Methods A total of 24 systemically healthy non-smokers, 12 periodontally healthy and 12 with SIII-C-P, were included in the study. Initially, GCF samples were collected from all patients, and then periodontal clinical parameters including plaque index, gingival index, bleeding on probing, pocket depth, clinical attachment level were measured. In the SIII-C-P group, clinical parameter measurements and GCF sample collection were repeated 3 months after NSPT. IL-26 and IL-6 levels were determined using an enzyme-linked immunosorbent assay. Results All clinical parameters improved after NSPT in SIII-C-P group (p<0.05). At baseline GCF IL-26 and IL-6 levels were higher in the SIII-C-P group than in the healthy group (p<0.05). Statistically significant decreases were detected in GCF IL-26 and IL-6 in the SIII-C-P group after NSPT (p<0.05). There was a positive correlation between IL-26 and IL-6 and all clinical parameters at baseline (p<0.01). Furthermore, IL-26 and IL-6 were positively correlated both at baseline and at 3 months after NSPT (p<0.05). Conclusions The present study revealed that GCF IL-26 level was higher in individuals with SIII-C-P and was associated with periodontal clinical parameters. Also, the NSPT was decreased the GCF IL-26 levels.

Keywords

• Gingival crevicular fluid
• interleukins
• periodontitis

Cerrahi Olmayan Periodontal Tedavinin Evre III Derece C Periodontitisli Hastalarda Dişeti Oluğu Sıvısındaki İnterlökin- 26 Seviyesine Etkisi

Abstract

Amaç Bu çalışmanın amacı, evre III (EIII) derece C periodontitis (C-P) ve sağlıklı bireylerde diş eti oluğu sıvısı (DOS) interlökin (İL) 26 ve İL-6 seviyelerini saptamak ve EIII-C-P hastalarda cerrahi olmayan periodontal tedavinin (COPT) İL-26 ve İL-6 seviyeleri üzerine etkisini değerlendirmektir. Gereç ve Yöntemler Çalışmaya, periodontal olarak sağlıklı 12 birey ve EIII-C-P’li 12 hasta olmak üzere, sistemik olarak sağlıklı, sigara içmeyen toplam 24 birey dahil edildi. Başlangıçta, tüm hastalardan DOS örnekleri toplandı ve ardından plak indeks, gingival indeks, sondalamada kanama, cep derinliği, klinik ataşman seviyesi parametrelerini içeren periodontal klinik parametreler ölçüldü. EIII-C-P’li grupta, COPT'den 3 ay sonra klinik parametrelerin ölçümleri ve DOS örneği toplanması tekrarlandı. İL-26 ve İL-6 seviyeleri, enzyme-linked immunosorbent assay kullanılarak belirlendi. Bulgular EIII-C-P grubunda COPT sonrası tüm klinik parametrelerde düzelme meydana geldi (p<0.05). Başlangıç DOS İL-26 ve İL-6 seviyeleri, EIII-C-P grubunda sağlıklı gruba göre daha yüksek bulundu (p<0.05). EIII-C-P grubunda COPT'den sonra, DOS İL-26 ve İL-6 seviyelerinde anlamlı azalma tespit edildi (p<0.05). Başlangıç İL-26 ve İL-6 ile tüm klinik parametreler arasında pozitif korelasyon saptandı (p<0.01). İL-26 ve İL-6 arasında ise hem başlangıçta hem de tedavi sonrası 3. ayda pozitif korelasyon tespit edildi (p<0.05). Sonuç Çalışmamız, DOS İL-26 seviyesinin EIII-C-P’li hastalarda daha yüksek olduğunu ve periodontal klinik parametrelerle ilişkili olduğunu ortaya koydu. Ayrıca, DOS İL-26 seviyesinde COPT ile azalma elde edildi.

Keywords

• Diş eti oluğu sıvısı
• interlökin
• periodontitis

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