Trigeminal Nevralji Hastalığında Serotonin 2A Reseptör Geni 5-HTR2A rs6311 Polimorfizminin Araştırılması

Abstract

Amaç: Trigeminal nevralji (TN), trigeminal sinirin dağılım bölgesinde ortaya çıkan ve sıklıkla tekrarlayan ağrılarla karakterize bir hastalıktır. Serotonin (5-HT), merkezi sinir sistemindeki nörotransmitterlere etki eder. Çalışmada, TN'yi genetik olarak inceleyerek hastalarda serotonin reseptör geni (5-HTR2A) duyarlılığı ve ağrı şiddeti ile rs6311 polimorfizmi arasındaki ilişkiyi araştırmak amaçlanmıştır. Gereç ve Yöntem: Çalışmada 10 TN hastası ve 10 sağlıklı birey yer almıştır. Kanlardan izole edilen DNA, StepOnePlus cihazında Real-Time PCR ve Taqman SNP Genotipleme Testleri kullanılarak 5-HTR2A rs6311 polimorfizminin genotiplenmesi üretici protokollerine göre yapıldı. Bulgular: 5HTR2A rs6311 analizinde, 10 TN hastasından 3'ünde CC, 5'inde CT ve 2'sinde TT genotipinin varlığı tespit edildi. Kontrol grubunda ise 4 kişide CC genotipi, 4 kişide CT genotipi ve 2 kişide TT genotipi tespit edildi. Sonuç: Hasta ve kontrol grupları arasındaki TN genotipi frekans farklılıklarının anlamlı olmadığı görüldü. Ancak CT genotipinin diğerlerine göre daha yaygın olduğu görüldü.

Keywords

• Trigeminal nevralji
• polimorfizm
• serotonin
• serotonin reseptörü

Investigation of Serotonin 2A Receptor Gene 5-HTR2A rs6311 Polymorphism in Trigeminal Neuralgia Disease

Abstract

Objectives: Trigeminal neuralgia (TN) is a disease that occurs in the distribution region of the trigeminal nerve and is frequently recurrent pain. Serotonin (5-HT) acts on neurotransmitters in the central nervous system. The study aims to examine the relationship between sensitivity and pain intensity of the serotonin receptor gene (5-HTR2A) and rs6311 polymorphism in patients by examining TN genetically. Materials and Methods: The study consisted of 10 TN patients and 10 healthy individuals. Genotyping of 5-HTR2A rs6311 polymorphism using DNA isolated from blood, Real-Time PCR in StepOnePlus device, and Taqman SNP Genotyping Tests were performed by manufacturers' protocols. Results: In 5HTR2A rs6311 analysis, 3 of 10 TN patients were found to have CC, 5 had CT and 2 had TT genotype. In the control group, CC genotype was detected in 4 individuals, CT genotype in 4 individuals, and TT genotype in 2 individuals. Conclusion: It was found that the TN genotype and allelic frequency differences between the patient and control groups were not significant. However, the CT genotype was found to be more common.

Keywords

• Trigeminal neuralgia
• polymorphism
• serotonin
• serotonin receptor

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