Effects of Methacrylate Exposure on Developing Zebrafish Embryos

Abstract

Aim:Methacrylate (MA), is widely used as a monomer in dentistry as well as medicine. MAderivatives have a long established role in biomedical devices and are utilized in restorative dental composites, contact lens materials, and bone cement. Zebrafish is one of the most commonly used fish species in toxicity potential testing. Aim of this study was to evaluate the effects of a MA derivative, polyethylmethacrylate (PEMA) exposure on the development of zebrafish embryo.

Materials and Method:Adult AB strain zebrafish were kept in an aquarium rack system (Zebtec, Tecniplast, Italy) at 27 ± 1 °C under a light/dark cycle of 14/10 h. Normally dividing and spherical embryos were selected and utilized for all of the described studies. After range-finding experiments zebrafish embryos were exposed to MA in well plates containing 20 embryos, having four replicates. Developmental effects and mortality rate were evaluated for 120h. Nitric oxide (NO) levels of the embryos were evaluated using Griess Method. 

Results:In present study no significant difference was observed in the NO levels of the embryos in the control group and in the MA exposed group. However, some developmental defects were observed in some of the MA exposed embryos. Lack of pigmentation was evident in one DMSO exposed embryo and pericardial edema was observed in some of the MA exposed embryos.

Conclusions: Our findings suggest that zebrafish embryos are useful models for the assessment of toxicity of dental MA and further research is needed to determine the potential effects of MA exposure in embryos.

Keywords

• Methacrylate
• zebrafish
• embryo

References

1. 1- Holmes PF, Bohrer M, Kohn J. Exploration of polymethacrylate structure-property correlations: Advances towards combinatorial and high-throughput methods for biomaterials discovery. Prog Polym Sci 2008;33(8):787–796.
2. 2- Gautam R, Singh RD, Sharma VP, Siddhartha R, Chand P, Kumar R. Biocompatibility of polymethylmethacrylate resins used in dentistry. J Biomed Mater Res B Appl Biomater 2012; 100(5):1444-1150.
3. 3- Ünal İ, Emekli-Alturfan E. Fishing for Parkinson's Disease: A review of the literature. J Clin Neurosci 2019; 62:1-6.
4. 4- Üstündağ ÜV, Ünal İ, Ateş PS, Alturfan AA, Yiğitbaşı T, Emekli-Alturfan E. Bisphenol A and di(2-ethylhexyl) phthalate exert divergent effects on apoptosis and the Wnt/β-catenin pathway in zebrafish embryos: A possible mechanism of endocrine disrupting chemical action. Toxicol Ind Health 2017; Dec;33(12):901-910.
5. 5- Litwack G. Preface. Nitric oxide. Vitamines Hormones 2014;96:xvii-xviii.
6. 6- Miranda KM, Espey MG, Wink DA. A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide 2001;5(1):62-71.
7. 7- Gosavi SS, Gosavi SY, Alla RK. Local and systemic effects of unpolymerised monomers. Dent Res J (Isfahan) 2010;7(2):82–87.
8. 8- Fakhouri J, Sarkis R, Chababi-Atallah M, Aftimos G. Toxic effects of methyl methacrylate monomer on male genital tissues. In vitro study in rats. J Med Liban 2008;56(1):22–26.

9. 9- Keyf F, Keyf I. Harmful effects of methylmethacrylate and formaldehyde from acrylic resin denture base materials. Saudi Dental Journal 1998;10(1):23–8.
10. 10- Leggat PA, Kedjarune U. Toxicity of methyl methacrylate in dentistry. Int Dent J 2003;53(3):126–131.
11. 11- Habib S, Ali A. Biochemistry of nitric oxide. Indian J Clin Biochem 2011;26(1):3–17.
12. 12- Miura GI, Yelon D. A guide to analysis of cardiac phenotypes in the zebrafish embryo. Methods Cell Biol 2011;101:161–180.