Klorheksidinin İnsan Dişeti Fibroblastları Üzerindeki Hücre Canlılığı ve Sitotoksisite Etkinliğinin İn vitro Koşullarda Değerlendirilmesi

Year 2023, Volume: 7 Issue: 1, 27 - 32, 30.04.2023

Gözdem Bayraktar , Ayse Yilmaz , Hafize Öztürk Özener

https://doi.org/10.29228/erd.40

Abstract

Amaç: Bu in vitro çalışmada, % 0,2’lik klorheksidin (CHX) solüsyonunun, insan dişeti fibroblastları (HGF) üzerindeki hücre canlılığı ve sitotoksisite etkinliklerinin değerlendirilmesi amaçlanmıştır.
Gereç ve Yöntemler: 30 saniye ve 2 dakikalık zaman aralıklarında, nötr pH değerindeki % 0,2’lik CHX solüsyonu kullanılarak çalışılmıştır. Hücre canlılığı ve sitotoksisitesi, HGF’ye karşı 3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolyum bromür (MTT) testi ve laktat dehidrogenaz (LDH) testi kullanılarak değerlendirilmiştir. Sonuçlar ortalama ve standart sapma değerleri kullanılarak two way ANOVA testiyle istatistiksel olarak değerlendirilmiştir (p<0,05).
Bulgular: MTT testi sonuçlarına göre, % 0,2 CHX solüsyonunun zamanla hücre canlılığını azalttığı gözlendi. LDH testi sonuçlarına göre ise % 0,2 CHX’in sitotoksik etkinliğinin kontrol grubuna kıyasla arttığı ancak zaman içinde istatistiksel olarak anlamlı bir fark göstermediği gözlendi.
Sonuç: Bu in vitro çalışmanın sınırları dahilinde, % 0,2 CHX solüsyonu, 30 sn ve 2 dk’lık kısa maruz kalma sürelerinde, HGF hücre canlılığını azalttı ve HGF üzerine sitotoksik etkinlik gösterdi.

Keywords

klorheksidin, fibroblast, in vitro, sitotoksisite, hücre canlılık testleri

Supporting Institution

Marmara Üniversitesi Bilimsel Araştırma Projeleri Komisyonu

Project Number

TDK-2021-10281

In vitro Evaluation of Cell Viability and Cytotoxicity Effects of Chlorhexidine on Human Gingival Fibroblasts

Abstract

Objectives: The aim of this study was to evaluate the cell viability and cytotoxicity effects of a 0.2 % chlorhexidine (CHX) solution on human gingival fibroblasts (HGF) in vitro.
Materials and Methods: A 0.2 % CHX solution with a neutral pH was tested at 30 second and 2 minute intervals. Cell viability and cytotoxicity against HGF were determined using the 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test and the lactate dehydrogenase (LDH) test. Results were evaluated by calculating means and standard deviations using the two-way ANOVA test (p<0.05).
Results: According to the MTT test results, it was observed that a 0.2 % CHX solution decreased cell viability over time. According to the LDH test results, the cytotoxic efficacy of 0.2 % CHX was higher compared to the control group, but did not show a statistically significant difference over time.
Conclusions: Within the limits of this in vitro study, 0.2 % CHX solution reduced HGF cell viability and showed cytotoxic activity on HGF during short exposure times of 30 sec and 2 min.

Keywords

Chlorhexidine, fibroblasts, in vitro, cytotoxicity, cell viability assays

Project Number

TDK-2021-10281

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