Doğu Anadolu Bölgesinde Kemik İliği ve Böbrek Nakli İçin Başvuran KBY’li Hastaların İnsan Lökosit Antijen Allel ve Haplotip Frekansı Dağılımı
Abstract
Amaç: Bu çalışmada, Doğu Anadolu Bölgesinde yaşayan ve Atatürk Üniversitesi Tıp Fakültesi Tıbbi Biyoloji Anabilim Dalı Doku Tipleme Laboratuvarına başvuran, kemik iliği (Kİ) ve kronik böbrek yetmezliği (KBY) sebebiyle böbrek nakli bekleyen hastaların HLA allellerinin dağılımı ve haplotip frekanslarının
belirlenmesi amaçlanmıştır.
Yöntemler: Çalışmamızda 1000 hastanın, periferal kanlarından PCR-SSP (Polimeraz Zincir Reaksiyonu- Sekansa Özgü Primer) ve PCR-SSO (Polimeraz Zincir Reaksiyonu-Sekansa Özgü
Oligonükleotit) yöntemleri kullanılarak HLA-A-B-DR doku tiplemeleri elde edilmiştir. 2011-2019 yılları arasındaki doku tipleme laboratuvar sonuçları geriye dönük olarak taranıp, HLA allelleri ve haplotip geçişlerini içeren bir tablo oluşturuldu, bölgemizdeki HLA Sınıf I ve HLA Sınıf II moleküllerinin birlikte geçiş frekansları belirlendi.
Bulgular: HLA-A lokusunda A*24 (%19,3), A*02 (%19,2), HLA-B lokusunda B*35 (%19), B*51 (%17,5), HLA-DRB1 lokusunda DRB1*11 (%23), DRB1*04 (%15,5) en sık görülen alleller oldu. A*02-B*35-DRB1*04 (%1,4), A*24-B*51-DRB1*11 (%1,3) ve A*02-B*35-DRB1*11 (%1,2) ise en sık görülen haplotipler olarak saptandı.
Sonuç: Araştırmanın neticesinde; heterojen etnik alt grupların olduğu Erzurum ili ve Doğu Anadolu bölgesinde yaşayan 1000 hastanın HLA allel frekansları ve haplotip geçişleri tespit edilmiş oldu. Böylelikle bölgemizde en fazla tekrar eden allel ve haplotip geçişleri belirlendi. Çalışmamızın Türkiye’de yapılan diğer çalışmaları destekler nitelikte olduğu ve literatüre katkı sağladığı
gözlemlendi.
References
- Akçam, F. Z. (2005). HLA Sistemi. Türkiye Klinikleri Tıp Bilimleri. Dergisi, 25, 829–834.
- Akman, B. (2016). Tepecik Eğitim ve Araştırma Hastanesi doku tipleme laboratuvarına başvuran bireylerin insan lökosit antijen (HLA) allellerinin ve haplotiplerinin frekansları. (Yüksek Lisans Tezi), İzmir Kâtip Çelebi Üniversitesi, Sağlık Bilimleri Enstitüsü.
- Ameen, R., Al Shemmari, S. H., & Marsh, S. G. E. (2020). HLA haplotype frequencies and genetic profiles of the Kuwaiti population. Medical Principles and Practice, 29(1), 39–45. [CrossRef]
- Chinen, J., & Buckley, R. H. (2010). Transplantation immunology: Solid organ and bone marrow. Journal of Allergy and Clinical Immunology, 125(2), S324–S335. [CrossRef]
- Everly, M. J., Rebellato, L. M., Haisch, C. E., Ozawa, M., Parker, K., Briley, K. P., Catrou, P. G., Bolin, P., Kendrick, W. T., Kendrick, S. A., Harland, R. C., & Terasaki, P. I. (2013). Incidence and impact of de novo donorspecific alloantibody in primary renal allografts. Transplantation, 95(3), 410–417. [CrossRef]
- Immuno Polymorphism Database. (2022). http: //www .ebi. ac.uk /imgt /hla/stats .html . Mayıs, 30.
- Kayhan, B., Kurtoglu, E., Taskapan, H., Piskin, T., Sahin, I., Otlu, G., & Unal, B. (2013). In HLA-A.-B,-DRB1 allele and haplotype frequencies and comparison with blood group antigens in dialysis patients in the east Anatolia region of Turkey. Transplantation Proceedings, 2123–2128.
- Lachmann, N., Terasaki, P. I., Budde, K., Liefeldt, L., Kahl, A., Reinke, P., Pratschke, J., Rudolph, B., Schmidt, D., Salama, A., & Schönemann, C. (2009). Anti-human leukocyte antigen and donor-specific antibodies detected by Luminex posttransplant serve as biomarkers for chronic rejection of renal allografts. Transplantation, 87(10), 1505–1513. [CrossRef]
- Mahdi, B. M. (2013). A glow of HLA typing in organ transplantation. Clinical and Translational Medicine, 2(1), 6. [CrossRef]
- Marsh, S. G. (2014). Nomenclature for factors of the HLA system, update March 2014. Tissue Antigens, 83(6), 444–453. [CrossRef]
- Opelz, G., & Döhler, B. (2007). Effect of human leukocyte antigen compatibility on kidney graft survival: Comparative analysis of two decades. Transplantation, 84(2), 137–143. [CrossRef]
- Özbolat, G., Yenilmez, E. D., & Abdulah, T. (2014). İnsan Lökosit Antijenleri, Yapı ve İşlevleri. Arşiv Kaynak Tarama Dergisi, 23, 387–397.
- Ramosaj-Morina, A., Burek Kamenaric, M., Keka Syla, A., Baloku, A., Grubic, Z., & Zunec, R. (2021). HLA-A,-C.,& -B.-DRB1,-DQA1, and-DQB1 allele and haplotype repertoires in the Albanian population from Kosovo. Immunological Investigations, 1–11.
- Sanchez‐Mazas, A., Fernandez‐Viña, M., Middleton, D., Hollenbach, J. A., Buhler, S., Di, D., Rajalingam, R., Dugoujon, J. M., Mack, S. J., & Thorsby, E. (2011). Immunogenetics as a tool in anthropological studies. Immunology, 133(2), 143–164. [CrossRef]
- Sheldon, S., & Poulton, K. (2006). HLA typing and its influence on organ transplantation. Methods in Molecular Biology, 333, 157–174. [CrossRef]
- Thorogood, J., Van Houwelingen, J. C., Van Rood, J. J., Zantvoort, F. A., Schreuder, G. M., & Persijn, G. G. (1992). Factors contributing to longterm kidney graft survival in Eurotransplant. Transplantation, 54(1), 152–158. [CrossRef]
- Yılmaz, M. (2014). Doku Uygunluk Antijenleri. Arşiv Kaynak Tarama Dergisi, 23(1), 8–13. [CrossRef]
Abstract
Objective: In this study, the distribution of human leukocyte antigen alleles of patients living in the Eastern Anatolia Region and applying to Atatürk University Medical Faculty Medical Biology Department Tissue Typing Laboratory and waiting for kidney transplantation due to bone marrow and chronic kidney failure are investigated and haplotype frequencies are determined.
Methods: In our study, human leukocyte antigen-A, B, DR tissue typing was done by using peripheral bloods obtained from 1000 patients using PCR-SSP (Polymerase Chain Reaction-Sequence Specific Primer) and PCR-SSO (Polymerase Chain Reaction-Sequence Specific Oligonucleotide) tissue typing analysis methods. Tissue typing laboratory results between 2011 and 2019 were retrospectively scanned, and a table containing human leukocyte antigen alleles and haplotype transitions was created.
Results: By using the data in the table, the crossover frequencies of human leukocyte antigen Class I and HLA Class II molecules in our region were determined. As a result of the analyses, A*24 (19.3%) and A*02 (19.2%) are the most common alleles at the human leukocyte antigen-A locus. B*35 (19%) and B*51 (17.5%) are the most common alleles at the human leukocyte antigen -B locus. DRB1*11 (23%) and DRB1*04 (15.5%) are the most common alleles at the human leukocyte antigen-DRB1 locus. A*02-B*35-DRB1*04 (1.4%), A*24-B*51-DRB1*11 (1.3%), and A*02-B*35-DRB1*11 (%1.2) are detected to be the most common haplotypes.
Conclusion: As a conclusion of our research, it has been identified that there are some heterogeneous ethnic subgroups. Moreover, human leukocyte antigen allele frequencies and haplotype transitions of 1000 patients living in Erzurum province and Eastern Anatolia Region were detected. Thus, the most repetitive allele and haplotype transitions were determined in our region. It has been observed that our research supports other studies conducted in Turkey and contributes to the literature.
References
- Akçam, F. Z. (2005). HLA Sistemi. Türkiye Klinikleri Tıp Bilimleri. Dergisi, 25, 829–834.
- Akman, B. (2016). Tepecik Eğitim ve Araştırma Hastanesi doku tipleme laboratuvarına başvuran bireylerin insan lökosit antijen (HLA) allellerinin ve haplotiplerinin frekansları. (Yüksek Lisans Tezi), İzmir Kâtip Çelebi Üniversitesi, Sağlık Bilimleri Enstitüsü.
- Ameen, R., Al Shemmari, S. H., & Marsh, S. G. E. (2020). HLA haplotype frequencies and genetic profiles of the Kuwaiti population. Medical Principles and Practice, 29(1), 39–45. [CrossRef]
- Chinen, J., & Buckley, R. H. (2010). Transplantation immunology: Solid organ and bone marrow. Journal of Allergy and Clinical Immunology, 125(2), S324–S335. [CrossRef]
- Everly, M. J., Rebellato, L. M., Haisch, C. E., Ozawa, M., Parker, K., Briley, K. P., Catrou, P. G., Bolin, P., Kendrick, W. T., Kendrick, S. A., Harland, R. C., & Terasaki, P. I. (2013). Incidence and impact of de novo donorspecific alloantibody in primary renal allografts. Transplantation, 95(3), 410–417. [CrossRef]
- Immuno Polymorphism Database. (2022). http: //www .ebi. ac.uk /imgt /hla/stats .html . Mayıs, 30.
- Kayhan, B., Kurtoglu, E., Taskapan, H., Piskin, T., Sahin, I., Otlu, G., & Unal, B. (2013). In HLA-A.-B,-DRB1 allele and haplotype frequencies and comparison with blood group antigens in dialysis patients in the east Anatolia region of Turkey. Transplantation Proceedings, 2123–2128.
- Lachmann, N., Terasaki, P. I., Budde, K., Liefeldt, L., Kahl, A., Reinke, P., Pratschke, J., Rudolph, B., Schmidt, D., Salama, A., & Schönemann, C. (2009). Anti-human leukocyte antigen and donor-specific antibodies detected by Luminex posttransplant serve as biomarkers for chronic rejection of renal allografts. Transplantation, 87(10), 1505–1513. [CrossRef]
- Mahdi, B. M. (2013). A glow of HLA typing in organ transplantation. Clinical and Translational Medicine, 2(1), 6. [CrossRef]
- Marsh, S. G. (2014). Nomenclature for factors of the HLA system, update March 2014. Tissue Antigens, 83(6), 444–453. [CrossRef]
- Opelz, G., & Döhler, B. (2007). Effect of human leukocyte antigen compatibility on kidney graft survival: Comparative analysis of two decades. Transplantation, 84(2), 137–143. [CrossRef]
- Özbolat, G., Yenilmez, E. D., & Abdulah, T. (2014). İnsan Lökosit Antijenleri, Yapı ve İşlevleri. Arşiv Kaynak Tarama Dergisi, 23, 387–397.
- Ramosaj-Morina, A., Burek Kamenaric, M., Keka Syla, A., Baloku, A., Grubic, Z., & Zunec, R. (2021). HLA-A,-C.,& -B.-DRB1,-DQA1, and-DQB1 allele and haplotype repertoires in the Albanian population from Kosovo. Immunological Investigations, 1–11.
- Sanchez‐Mazas, A., Fernandez‐Viña, M., Middleton, D., Hollenbach, J. A., Buhler, S., Di, D., Rajalingam, R., Dugoujon, J. M., Mack, S. J., & Thorsby, E. (2011). Immunogenetics as a tool in anthropological studies. Immunology, 133(2), 143–164. [CrossRef]
- Sheldon, S., & Poulton, K. (2006). HLA typing and its influence on organ transplantation. Methods in Molecular Biology, 333, 157–174. [CrossRef]
- Thorogood, J., Van Houwelingen, J. C., Van Rood, J. J., Zantvoort, F. A., Schreuder, G. M., & Persijn, G. G. (1992). Factors contributing to longterm kidney graft survival in Eurotransplant. Transplantation, 54(1), 152–158. [CrossRef]
- Yılmaz, M. (2014). Doku Uygunluk Antijenleri. Arşiv Kaynak Tarama Dergisi, 23(1), 8–13. [CrossRef]
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Health Care Administration |
| Journal Section | Articles |
| Authors | |
| Publication Date | December 30, 2022 |
| Submission Date | June 1, 2022 |
| Acceptance Date | September 8, 2022 |
| Published in Issue | Year 2022 |
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