Clinical Significance of The New Beckman-Coulter Parameters in the Diagnosis of Iron Deficiency Anemia
Abstract
Summary:Iron deficiency anemia (IDA) is the most common form of nutritional anemia worldwide. Despite the availability of various biochemical parameters for its diagnosis, their validity is still debatable. Therefore a simple, cheap and useful tool for diagnosis of IDA is under research. The aim of this study was to find the threshold values of the new Beckman-Coulter parameters in Turkish population, show their utility in the diagnosis of IDA and compare them with other conventional iron parameters.
Materials and Methods: A total of 115 female patients with iron deficiency anemia (serum hemoglobin <120 g/L and serum ferritin <20ng/ml), 55 female patients with iron deficiency (serum hemoglobin >120 g/L and serum ferritin <20 ng/ml) and 165 female with healthy individuals were enrolled.
Findings: Red blood cell size factor (RSf) and microcytic anemia factor (MAF) showed a significant positive correlation with hemoglobin, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean cell hemoglobin concentration (MCHC), serum iron, transferrin saturation and a significant negative correlation with transferrin and total iron binding capacity. Low hemoglobin density (LHD) showed a significant negative correlation with hemoglobin, MCV, MCH, MCHC, serum iron, transferring saturation and a significant positive correlation with transferrin and total iron binding capacity. The receiver-operating characteristic (ROC) curve analysis demonstrated 92.45 fl, 6.71% and 10.14 for RSf, LHD and MAF, respectively.
Discussion: Our data demonstrated that these parameters are useful and can be confidentially used in the diagnosis of IDA.
References
- 1. Goddard AF, McIntyreAS, Scott BB. Guidelines for the management of iron deficiency anaemia. Gut 2000; 46(Suppl IV):1-5.
- 2. Fishbane S, Galgano C, Langley RC Jr, Canfield W, Maesaka JK. Reticulocyte hemoglobin content in the evaluation of iron status of haemodialysis patients. KidneyInt 1997; 52: 217-222.
- 3. Bridges KR, Seligman PA. Disorders of iron metabolism. In: Blood: Principles&Practice of Hematology, Handin RI, Lux SE, Stossel TP (Eds), 1995. p.ch.49.
- 4. Zafer B. Anemili Hastada Ayırıcı Tanı Rehberi. İ.Ü. Cerrahpaşa Tıp Fakültesi Sürekli Tıp Eğitimi Etkinlikleri Anemiler Sempozyumu; 2001 19-20 Nisan; İstanbul, Türkiye;2001. s. 107-116
- 5. Birsen B. Demir Eksikliği Anemisi: Klinik Hematolojinin ABC’si. İ.Ü. Cerrahpaşa Tıp Fakültesi Sürekli Tıp Eğitimi Etkinlikleri Anemiler Sempozyumu; 2001 19-20 Nisan; İstanbul, Türkiye; 2001. s. 23-32
- 6. Finch CA, Bellotti V, Stray S , Lipschitz DA: Plasma ferritin determination as a diagnostic tool . West Ind J Med 1986, 145:657-663
- 7. Allan B Schartz, Vinodprasad Jaslot Garcha. Anemia of chronic Kidney Disease: A combine deffect of marginal iron stores and erythropoeitin deficiency. Nephrol Dialysis and Transplantation 2004. Vol 33 No 12, pp 758-767
- 8. Lipschitz DA,Cook JD,Finch CA;A clinical evaluation of serum ferritin as an index of iron stores. New Eng J Med 1974, 290;1213-1216
- 9. Burhan F. Anemide Çevresel Kanın İncelenmesi. İ.Ü. Cerrahpaşa Tıp Fakültesi Sürekli Tıp Eğitimi Etkinlikleri Anemiler Sempozyumu; 2001 19-20 Nisan; İstanbul, Türkiye;2001. s. 17-22
- 10. Urrechaga E. Clinical utility of thenew beckman-coulter parameter red blood cell size factor in the study of erithropoiesis. International J of LaboratoryHematology 2009, 31, 623-629
- 11. Urrechaga E, Borque L, Escanero JF. Erythrocyte and ReticulocyteIndices on the LH750 as Potential Markers of Functional Iron Deficiency. Anemia 2010,doi: 10.155/2010/625919
- 12. Osta V, Caldirola MS, Fernandez M, Marcone MI, Tıssera G, Pennesi S, Ayuso C. Utility of new mature erythrocyte and reticulocyte indices in screening for iron-deficiency anemia in a pediatric population. International J of Laboratory Hematology 2013; 35(4):400-5
- 13. Dopsaj V, Mikovic-Golubovic G, Martinovic J, Kalimanovska-Ostric D: Evaluation of derived Coulter red blood cell parameters for the assesment of iron eficiency in adults with the congenital heart disease. International J of Laboratory Hematology 2012,34,461-472
- 14. Esda H Y Ng, Junior H W L, Lau Y S, Edmond S K Ma. Evaluation of new red cell parameters on BeckmanCoulter DxH800 in the detection of iron deficiency and thalasaemia carrier. International Society for Laboratory Hematology 2013
- 15. Oustamanolakis P, Koutroubakis I E, Messaritakis I, Kefalogiannis G, Niniraki M, Kouromalis EA. Measurement of reticulocyte and red blood cell indices in the evaluation of anemia in inflammatory bowel disease. Journal of Crohn’s and Colitis 2011:5, 295-300
- 16. Fishbane S and et al. The evaluation of iron status in patients receiving recombinant human erythropoietin. Journal of American Society of Nephrology, vol.7, pp.654-657,1996
- 17. Urrechaga E. The new mature red cell parameter, low hemoglobin density of the Beckman-Coulter LH 750: clinical utility in the diagnosis of iron deficiency. International J of Laboratory Hematology 2010, 32, 144-150
- 18. Dopsaj V, Martinovic J, Dopsaj M. Early detection of iron deficiency in elite athletes: could microcytic anemia factor (Maf) be useful? International J of Laboratory Hematology 2014; 36(1):pp 37-44
Abstract
Özet: Demir Eksikliği Anemisi (DEA) tanısında yeni Beckman-Coulter parametreleri kolay, ucuz ve faydalı tetkiklerdir. Bu çalışmanın amacı, Türk halkında DEA tanısında yeni Beckman-Coulter parametreleri olan Red cell size factor (RSf), low hemoglobin density (LHD) ve Microcytic Anemia factor (MAF)’ın klinik önemini belirlemek ve diğer konvansiyonel demir parametreleri ile karşılaştırmaktır.
Gereç ve Yöntemler: Çalışmaya demir eksikliği anemisi olan 155 kadın hasta ve demir eksikliği olan 55 kadın hasta ve 165 sağlıklı kontrol dahil edildi.
Bulgular: RSf ve MAF; hemoglobin, MCV, MCH, MCHC, serum demiri ve transferrin saturasyonu ile anlamlı pozitif korelasyon; transferrin ve total demir bağlama kapasitesi ile anlamlı negatif korelasyon gösterdi. LHD ise; hemoglobin, MCV, MCH, MCHC, serum demiri ve transferrin saturasyonu ile anlamlı negatif korelasyon; transferrin ve total demir bağlama kapasitesi ile anlamlı pozitif korelasyon gösterdi DEA tanısında ROC analizi ile belirlenen cut-off değerler: RSf için 92.45 fl, LHD için % 6.71 ve MAF için 10.14 olarak belirlendi.
Sonuç: Bizim verilerimiz, bu parametrelerin DEA tanısında güvenle kullanılabilecek parametreler olduğunu göstermiştir.
Keywords
References
- 1. Goddard AF, McIntyreAS, Scott BB. Guidelines for the management of iron deficiency anaemia. Gut 2000; 46(Suppl IV):1-5.
- 2. Fishbane S, Galgano C, Langley RC Jr, Canfield W, Maesaka JK. Reticulocyte hemoglobin content in the evaluation of iron status of haemodialysis patients. KidneyInt 1997; 52: 217-222.
- 3. Bridges KR, Seligman PA. Disorders of iron metabolism. In: Blood: Principles&Practice of Hematology, Handin RI, Lux SE, Stossel TP (Eds), 1995. p.ch.49.
- 4. Zafer B. Anemili Hastada Ayırıcı Tanı Rehberi. İ.Ü. Cerrahpaşa Tıp Fakültesi Sürekli Tıp Eğitimi Etkinlikleri Anemiler Sempozyumu; 2001 19-20 Nisan; İstanbul, Türkiye;2001. s. 107-116
- 5. Birsen B. Demir Eksikliği Anemisi: Klinik Hematolojinin ABC’si. İ.Ü. Cerrahpaşa Tıp Fakültesi Sürekli Tıp Eğitimi Etkinlikleri Anemiler Sempozyumu; 2001 19-20 Nisan; İstanbul, Türkiye; 2001. s. 23-32
- 6. Finch CA, Bellotti V, Stray S , Lipschitz DA: Plasma ferritin determination as a diagnostic tool . West Ind J Med 1986, 145:657-663
- 7. Allan B Schartz, Vinodprasad Jaslot Garcha. Anemia of chronic Kidney Disease: A combine deffect of marginal iron stores and erythropoeitin deficiency. Nephrol Dialysis and Transplantation 2004. Vol 33 No 12, pp 758-767
- 8. Lipschitz DA,Cook JD,Finch CA;A clinical evaluation of serum ferritin as an index of iron stores. New Eng J Med 1974, 290;1213-1216
- 9. Burhan F. Anemide Çevresel Kanın İncelenmesi. İ.Ü. Cerrahpaşa Tıp Fakültesi Sürekli Tıp Eğitimi Etkinlikleri Anemiler Sempozyumu; 2001 19-20 Nisan; İstanbul, Türkiye;2001. s. 17-22
- 10. Urrechaga E. Clinical utility of thenew beckman-coulter parameter red blood cell size factor in the study of erithropoiesis. International J of LaboratoryHematology 2009, 31, 623-629
- 11. Urrechaga E, Borque L, Escanero JF. Erythrocyte and ReticulocyteIndices on the LH750 as Potential Markers of Functional Iron Deficiency. Anemia 2010,doi: 10.155/2010/625919
- 12. Osta V, Caldirola MS, Fernandez M, Marcone MI, Tıssera G, Pennesi S, Ayuso C. Utility of new mature erythrocyte and reticulocyte indices in screening for iron-deficiency anemia in a pediatric population. International J of Laboratory Hematology 2013; 35(4):400-5
- 13. Dopsaj V, Mikovic-Golubovic G, Martinovic J, Kalimanovska-Ostric D: Evaluation of derived Coulter red blood cell parameters for the assesment of iron eficiency in adults with the congenital heart disease. International J of Laboratory Hematology 2012,34,461-472
- 14. Esda H Y Ng, Junior H W L, Lau Y S, Edmond S K Ma. Evaluation of new red cell parameters on BeckmanCoulter DxH800 in the detection of iron deficiency and thalasaemia carrier. International Society for Laboratory Hematology 2013
- 15. Oustamanolakis P, Koutroubakis I E, Messaritakis I, Kefalogiannis G, Niniraki M, Kouromalis EA. Measurement of reticulocyte and red blood cell indices in the evaluation of anemia in inflammatory bowel disease. Journal of Crohn’s and Colitis 2011:5, 295-300
- 16. Fishbane S and et al. The evaluation of iron status in patients receiving recombinant human erythropoietin. Journal of American Society of Nephrology, vol.7, pp.654-657,1996
- 17. Urrechaga E. The new mature red cell parameter, low hemoglobin density of the Beckman-Coulter LH 750: clinical utility in the diagnosis of iron deficiency. International J of Laboratory Hematology 2010, 32, 144-150
- 18. Dopsaj V, Martinovic J, Dopsaj M. Early detection of iron deficiency in elite athletes: could microcytic anemia factor (Maf) be useful? International J of Laboratory Hematology 2014; 36(1):pp 37-44
Details
| Primary Language | English |
|---|---|
| Subjects | Health Care Administration |
| Journal Section | Research Articles |
| Authors | |
| Publication Date | November 30, 2022 |
| Published in Issue | Year 2022 Volume: 3 Issue: 3 |
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