DETERMINATION OF THE CONTENT OF HYOSCYAMUS RETICULATUS SEEDS BY XRF METHOD AND ANTIBACTERIAL CHARACTERISTICS OF ETHYL ALCOHOL EXTRACT

Year 2020, Volume: 21 Issue: 1, 199 - 204, 31.03.2020

Perihan Akbaş , Elife Kaya , Hicran Alkan Gökhan Ceylan

https://doi.org/10.18038/estubtda.622224

Cited By: 2

Abstract

Hyoscyamus reticulatus is a member of the Solanaceae family and is
represented by six species in Turkey. Hyoscyamus species contain important
tropane alkaloids, which have antispasmodic, anticholinergic, analgesic and
sedative effects. In this study, we aimed to determinate content of Hyoscyamus reticulatus plant seed by XRF
(X-ray Fluorescence Spectrometry) and investigate the antibacterial activity of
the ethanol extract of H. reticulatus seed.
Energy dispersive X-ray fluorescence (XRF) spectrometry is one of the important
instruments which used to determine the elemental and chemical composition of
all types of liquids and solids, allows quick and accurate qualitative and
quantitative analysis of elements. In this study, content of H. reticulatus plant seeds were
determined by XRF method; While the seeds have content rates % 1.98 K₂O
(%1.65 K), %1.22 P₂O₅ (%0.53 P), % 0.80 SO₃ (%
0.32 S), % 0.49 CaO (%0.35 Ca), %0.26 Fe₂O₃ ( %0.18
Fe), %0.13 Cl, %0.11 MgO (%0.07 Mg), %0.10 ZnO ( %0.08 Zn), %0.06 MoO₃ (
%0.04 Mo),%0.05 SiO₂ (%0.02 Si), %0.02 Cu, %0.02 MnO (%0.01
Mn), NiO (67 ppm) and Al₂O₃ (94 ppm) rates were
determined at the ppm level. H.
reticulatus seeds were extracted with for antibacterial activity test and Escherichia coli, Pseudomonas aeruginosa,
Pasteurella multocida, Yersinia enterocolitica, Klebsiella pneumoniae,
Staphylococcus aureus and Salmonella
enteridis bacteria were used. The extract did affect no bacteria except Pasteurella multocida (10 mm). 

Keywords

Hyoscyamus reticulatus seed extract, antibacterial, XRF

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