UTILIZATION OF FMN07 AND FGN37 MICROMONOSPORA SP. IN REGULATING THE NITROGEN LOSS DURING COMPOSTING

Abstract

Isolated and purified Micromonospora sp. FMN07 and FGN37 strains were utilized in composting experiments in a miniscule system in order to determine their effects on mesophilic stage. Results indicated deceleration of organic matter degradation in the presence of these microorganisms. This result also indicated that these microorganisms had no or negligible activity towards cellulose degradation which was the main constituent of compost mixture. Statistical analyses and measurements were also conducted to determine the effects of microorganisms on nitrogen content. Result indicated an increase in nitrogen amount in their presence. It was our belief that the activity of microorganisms effective in thermophilic stage of composting could have been increased by proper adjustment of FMN07 and FGN37 strains since nitrogen amount crucial for microorganism growth would have been higher in their presence.

Keywords

• Micromonospora,composting,nitrogen amaount

UTILIZATION OF FMN07 AND FGN37 MICROMONOSPORA SP. IN REGULATING THE NITROGEN LOSS DURING COMPOSTING

Abstract

References

1. [1] Pepe O, Ventorino V, Blaiotta G. Dynamic of functional microbial groups during mesophilic composting of agro-industrial wastes and free-living (N2)-fixing bacteria application. Waste Manage 2013; 33: 1616-1625.
2. [2] Zheng Y, Zhao Y, Chen Y, Lu Q, Li M, Wang X, Wei Y, Xie X, Wei Z. A regulating method for reducing nitrogen loss based on enriched ammonia-oxidizing bacteria during composting. Bioresource Technol 2016;221: 276-283.
3. [3] Iwata K, Azlan A, Yamakawa H, Omori T. Ammonia accumulation in culture broth by the novel nitrogen-fixing bacterium, Lysobacter sp. E4. J Biosci Bioeng 2010; 110(4): 415-418.
4. [4] Yates RJ, Howieson JG, Reeve WG, Nandasena KG, law IJ, Brau L, Ardley JK, Nistelberger HM, Real D, O’Hara GW. Lotononis angolensis forms nitrogen fixing, lupinoid nodules with phylogenetically unique, fast-growing, pink-pigmented bacteria, which do not nodulate L. bainesii or L. listii. Soil Biol Biochem 2007; 39: 1680-1688.
5. [5] Zhang Y, Cao C, Peng M, Xu X, Zhang P, Yu Q, Sun T. Diversity of nitrogen-fixing, ammonia-oxidizing, and denitrifying bacteria in biological soil crusts of a revegetation area in Horqin Sandy Land, Northeast China. Ecol Eng 2014; 71: 71-79.
6. [6] Yan Z, Wang G, Gao Y, Wang Y, Gu JD, Wang W. Diversity of ammonia-oxidizing bacteria and archaea in response to different aeration rates during cattle manure composting. Ecol Eng 2016; 93: 46-54.
7. [7] Meng L, li W, Zhang S, Wu C, Jiang W, Sha C. Effect of different extra carbon sources on nitrogen loss control and the change of bacterial populations in sewage sludge composting. Ecol Eng 2016; 94: 238-253.
8. [8] Chan MT, Selyam A, Wong JWC. Reducing nitrogen loss and salinity during ‘struvite’ food waste composting by zeolite amendment. Bioresource Technol. 2016; 200: 838-844.

9. [9] Nigussie A, Kuyper TW, Bruun S, de Neergaard A. Vermicomposting as a technology for reducing nitrogen losses and greenhouse gas emissions from small-scale composting. J Clean Prod 2016; 139: 429-439.
10. [10] Isik K, Gencbay T, Özdemir- Kocak F, Cil E. Molecular identification of different actinomycetes isolated from East Black Sea region plateau soil by 16S rDNA gene sequencing. Afr J Microbİol Res 2014; 8: 878-887.
11. [11] Ozdemir-Kocak F, Isik K. Molecular identification of Nocardia Diversity in soil by multilocus sequence analysis. BioDiCon 2015; 8(2): 122-133.
12. [12] Pitcher DG, Saunders NA, Owen RJ. Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett AppL Microbiol 1989; 8: 151-156.
13. [13] Lane DJ. 16S/23S rRNA sequencing. In E. Stackebrandt, M. Goodfellow, J. Wieley (eds) Nucleic acid techniques in bacterial systematics, New York pp. 1991; 115-175.
14. [14] Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol. 2013; 30: 2725–2729.
15. [15] Jukes TH, Cantor CR. Evolution of protein molecules. Academic Press, New York. 1969.
16. [16] Saitou N, Nei M. The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4: 406-425.
17. [17] ASTM. Standard test methods for moisture, ash, and organic matter of peat and other organic soils. American Society for Testing and Materials. West Conshohocken, PA. 2000.
18. [18] Barker AV, Volk RJ. Determination of Ammonium, Amide, Amino, and Nitrate Nitrogen in Plant Extracts by a Modified Kjeldahl Method. Anal Chem 1964; 36(2): 439-441.
19. [19] Artz RRE, Chapman SJ, Robertson AH, Potts JM, Laggoun-Defarge F. FTIR spectroscopy can predict organic matter quality in regenerating cutover peatlands. Soil Biol Biochem 2008; 40(2): 515-527.
20. [20] Merlin N, Lima VA, Santos-Tonial LM. Instrumental and Experimental Conditions for the Application of Fourier Transform Infrared Analysis on Soil and Humic Acid Samples, Combined with Chemometrics Tools and Scanning Electron Microscopy. J Brazil Chem Soc 2015; 26(9): 1920-1927.