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Azadirachtin’in model böcek Galleria mellonella (Lepidoptera: Pyralidae)'nın gelişimi üzerine etkileri

Year 2019, Volume: 8 Issue: 1, 85 - 91, 01.01.2019

Hülya Altuntaş Beyza Dere Zahide Ulya Nurullahoğlu

Abstract

Bitkisel köekenli bir allelokimyasal olan Azadirachtin (AZA), zararlı organizmalara karşı kullanılmakta ve Lepidopter türleri bu maddeye karşı oldukça hassastır. Çalışmamızda, model böcek büyük balmumu güvesi olarak bilinen Galleria mellonella L. (Lepidoptera: Pyralidae) larvalarına besleme yöntemi ile uygulanan AZA’nın biyolojik etkileri araştırıldı. Farklı dozlardaki teknik AZA (0,2 - 5 µg/larva)’nın etkilerinin araştırılması için 0.16 ± 0.01 gr ağırlığındaki larvalar seçildi ve böceklere ait zorla besleme yöntemi uygulandı. Sonuçlar AZA’nın farklı dozlarının G. mellonella’nın larval gelişim sürecinde letal ve subletal etkilerinin olduğunu gösterdi. Artan AZA dozlarına bağlı olarak larval yaşam süresinin uzadığını ve pupal yaşam süresinin değişmediği belirlendi. AZA dozlarına bağlı olarak ergin yaşam süresi, pupal ve ergin ağırlıkları azaldı. Sonuç olarak, bu çalışmada AZA'nın insektisidal aktiviteye sahip olduğu ve böceklerin yaşam süreleri üzerinde olumsuz etkilere neden olduğu model böcek G. mellonella kullanılarak gösterilmiştir.

Keywords

Azadirachtin Galleria mellonella model böcek gelişim süreleri

References

  • Hull L.A, Beers E.H. Ecological selectivity: Modifying chemical control practices to preserve natural enemies. In: Hoy MA, Herzog DC (eds) Biological Pest Control in Agricultural Ecosystem, Academic. Press, Orlando, Florida, pp 589, 1985.
  • Senthil-Nathan S. Physiological and biochemical effect of neem and other Meliaceae plants secondary metabolites against lepidopteran insects. Frontiers in Physiology 2013; 4, 1-17.
  • Biswas K, Chattopadhyay I, Banerjee R.K, Bandyopadhyay U. Biological activities and medicinal properties of neem (Azadirachta indica). Current Science 2002; 82:1336-1345.
  • Prakash G, Bhojwani S.S, Srivastava A.K. Production of azadirachtin from plant tissue culture: state of the art and future prospects. Biotechnol Bioproc Eng 2002; 7:185-193.
  • Isman M.B, Koul O, Luczynski A, Kaminski J. Insecticidal and antifeedant bioactivities of Neem oils and their relationship to azadirachtin content. J Agr Food Chem 1990; 38:1406-1411.
  • Schmutterer H. Properties and potential of natural pesticides from the Neem tree, Azadirachta indica. Ann Rev Entomol, 1990; 35:271-297.
  • Sanchez L.E, Osornio J.J, Herrera M.A. Secondary metabolites of the Annonaceae, Solanaceae and Meliaceae families used as biological control of insects. Tropic Subtropic Agroeco, 2010; 12:445-462.
  • Singh S. Effects of aqueous extract of neem seed kernel and azadirachtin on the fecundity, fertility and post-embriyonic development of the melonfly, Bactrocera cucurbitae and the oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae). J Appl Entomol 2003; 127:540-547.
  • Bruce Y.A, Gounou S, Olaye A.C, Smith H, Schulthess F. The effects of neem (Azadirachta indica A.Juss) oil on oviposition, development and reproductive potentials of Sesamia calamistis Hampson (Lepidoptera: Noctuidae) and Eldana saccharina Walker (Lepidoptera: Pyralidae). Agr Forest Entomol 2004; 6:223-232.
  • Bajwa A. A, Ahmad A. Potential applications of Neem based products as biopesticides. The Health 2012; 3:116-120.
  • Awad T. I, Onder F, Kismali S. Azadirachta indica A. Juss (Meliaceae) ağacından elde edilen doğal pestisitler üzerinde bir inceleme. Turk Entomol Derg 1998; 22 (3), 225- 240.
  • Bhowmik D, Chiranjib J.Y, Tripathi K.K, Kumar S. Herbal remedies of Azadirachta indica and its medicinal application. J Chem Pharm Res 2010; 2 (1), 62- 72.
  • Pankaj S, Lokeshwar T, Mukesh B, Vishnu B. Review on Neem (Azadirachta indica): thousand problems one solution. Int Res J Pharm 2011; 2 (12), 97- 102.
  • Barnby M. A, Klocke J.A. Effects of azadirachtin on the nutrition and development of the tobacco budworm, Heliothis virescens (Fabr.) (Noctuidae). J Insect Physiol 1987; l33:69.
  • Koul O. Biological activity of volatile D-n-Propyl Difulside from seeds of neem, Azadirachta indica (Meliaceae), to two species of stored grain pests, Sitophilus oryzae (L.) and Tribolium oryzae (Herbst). J Econ Entomol 2004; 97:1142-1147.
  • Dere B, Altuntaş H, Nurullahoğlu Z.U. Insecticidal and oxidative effects of azadirahtin on the model organism Galleria mellonella L. (Lepidoptera: Pyralidae). Arch Insect Biochem Physiol 2015; 89:138-52.
  • Mordue (Luntz) A.J, Nisbet A. Azadirachtin from the Neem Tree Azadirachta indica: its Action Against Insects. Ann Soc Entomol 2000; 29:615-632.
  • Malczewska M, Gelman S.B, Cymborowski B. Effect of azadirachtin on development, juvenile hormone and ecdysteroid titres in chilled Galleria mellonella larvae. J Insect Physiol 1988; 34:725-732.
  • Adel M. M, Sehnal F. Azadirachtin potentiates the action of ecdysteroid agoinst RH-2485 in Spodoptera littoralis. J Insect Physiol 2000; 46: 267-274.
  • Charriere J.D, Imdorf A. Protection of honey combs from moth damage. Liebefeld, Bern, Switzerland, 1997.
  • Rajendran S, Hajira Parveen K.M. Insect infestation in stored animal products. J Stor Product Res 2005; 41, 1–30.
  • Kwadha C.A, Ong’amo G.O, Ndegwa P.N, Raina S.K, Fombong A.T. The biology and control of the greater wax moth Galleria mellonella. Insects 2017; 8(61): 1-17.
  • Altınçiçek B, Lindner M, Linder D, Preissner K, Vilcinskas A. Microbial metalloproteinases mediate sensing of invading pathogens and activate innate immune responses in the lepidopteran model host Galleria mellonella. Infection and Immunity 2007; 75: 175-183.
  • Altuntaş H, Duman E, Şanal-Demirci S.N, Ergin E. Toxicological and physiological effects of ethephon on the model organism, Galleria mellonella L. 1758 (Lepidoptera: Pyralidae). Turk Entomol Derg 2016; 40(4), 413-423.
  • Maguire R, Duggan O, Kavanagh K. Evaluation of Galleria mellonella larvae as an in vivo model for assessing the relative toxicity of food preservative agents. Cell Biol Toxicol 2016; 32(3):209-16.
  • Bronskill J.K. A cage to simplify the rearing of greater wax moth, Galleria mellonella (Pyralidae). Journal of Lepidopterists’ Society 1961; 15, 102-104.
  • Metspalu L, Jogar K, Ploomi A, Hiiesaar K, Kivimagi I, Luik A. Effects of biopesticide Neem EC on the Mamestra brassicae L. (Lepidoptera, Noctuidae). Agronomy Research 2010; 8:465-470.
  • Erdoğan P, Toros S. Azadirachta indica A. Juss ekstraktlarının Patates böceği [Leptinotarsa decemlineata Say (Col.: Chrysomelidae)] larvalarının gelişimi üzerine etkisi. Bitki Koruma Bülteni, 2010; 50:73-88.
  • Er A, Taşkıran D, Sak O. Azadirachtin-induced effects on various life history traits and cellular immune reactions of Galleria mellonella (Lepidoptera: Pyralidae). Arch of Biolog Scien 2017; 69(2): 335-344.
  • Simmonds M.S, Jarvis A.P, Johnson S, Jones G.R, Morgan E.D. Comparison of anti-feedant and insecticidal activity of nimbin and salannin photooxidation products with neem (Azadirachta indica) limonoids. Pest Manag Scien 2004; 60, 459-464.
  • Wondafrash M, Getu E, Terefe G. Survival and feeding of African bollworm, Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) affected by Neem, Azadirachta indica (A. Juss) extracts. WJAS 2012; 8 (3): 280-285.
  • Jogar K, Kuusik A, Metspalu L, Hiiesaar K, Grishakova M, Luik A. Effects of neem EC on gas exchange, tracheal ventilation, and water loss in diapausing pupae of Pieris brassicae. The Authors Entomologia Experimentalis et Applicata, 2008; 126, 165-173.
  • Roel A.R, Dourado D.M, Matias R, Porto K.R, Bednaski A.V, Da Costa R.B. The effect of sub-lethal doses of Azadirachta indica (Meliaceae) oil on the midgut of Spodoptera frugiperda (Lepidoptera, Noctuidae). Revista Brasileira de Entomologia 2010; 54(3), 505-510.

EFFECTS OF AZADIRACHTIN ON DEVELOPMENT OF MODEL INSECT Galleria mellonella L. (LEPIDOPTERA: PYRALIDAE)

Year 2019, Volume: 8 Issue: 1, 85 - 91, 01.01.2019

Hülya Altuntaş Beyza Dere Zahide Ulya Nurullahoğlu

Abstract

Azadirachtin (AZA), a plant allelochemical, is used against pest organisms and lepidopteran species are quite sensitive to this substance. In this study, the developmental effects of AZA on model insect and greater wax moth, Galleria mellonella L. (Lepidoptera: Pyralidae) larvae via force feeding method were investigated. To investigate the effects of different doses of pure AZA (0.2 - 5 µg/larva), seventh instar larvae were fed by force feeding method. The results showed that all doses of AZA caused lethal and sublethal effects on G. mellonella. Larval developmental time increased while that of pupa did not change depending on the increasing doses of AZA. Adult longevity, and weights of pupae and adults decreased in at all AZA doses in a dose dependent manner. In conclusion, this study, using G. mellonella as a model organism, showed that AZA has insecticidal activity and causes adverse effects on the life history traits of G. mellonella.

Keywords

Azadirachtin Galleria mellonella model insect developmental time

References

  • Hull L.A, Beers E.H. Ecological selectivity: Modifying chemical control practices to preserve natural enemies. In: Hoy MA, Herzog DC (eds) Biological Pest Control in Agricultural Ecosystem, Academic. Press, Orlando, Florida, pp 589, 1985.
  • Senthil-Nathan S. Physiological and biochemical effect of neem and other Meliaceae plants secondary metabolites against lepidopteran insects. Frontiers in Physiology 2013; 4, 1-17.
  • Biswas K, Chattopadhyay I, Banerjee R.K, Bandyopadhyay U. Biological activities and medicinal properties of neem (Azadirachta indica). Current Science 2002; 82:1336-1345.
  • Prakash G, Bhojwani S.S, Srivastava A.K. Production of azadirachtin from plant tissue culture: state of the art and future prospects. Biotechnol Bioproc Eng 2002; 7:185-193.
  • Isman M.B, Koul O, Luczynski A, Kaminski J. Insecticidal and antifeedant bioactivities of Neem oils and their relationship to azadirachtin content. J Agr Food Chem 1990; 38:1406-1411.
  • Schmutterer H. Properties and potential of natural pesticides from the Neem tree, Azadirachta indica. Ann Rev Entomol, 1990; 35:271-297.
  • Sanchez L.E, Osornio J.J, Herrera M.A. Secondary metabolites of the Annonaceae, Solanaceae and Meliaceae families used as biological control of insects. Tropic Subtropic Agroeco, 2010; 12:445-462.
  • Singh S. Effects of aqueous extract of neem seed kernel and azadirachtin on the fecundity, fertility and post-embriyonic development of the melonfly, Bactrocera cucurbitae and the oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae). J Appl Entomol 2003; 127:540-547.
  • Bruce Y.A, Gounou S, Olaye A.C, Smith H, Schulthess F. The effects of neem (Azadirachta indica A.Juss) oil on oviposition, development and reproductive potentials of Sesamia calamistis Hampson (Lepidoptera: Noctuidae) and Eldana saccharina Walker (Lepidoptera: Pyralidae). Agr Forest Entomol 2004; 6:223-232.
  • Bajwa A. A, Ahmad A. Potential applications of Neem based products as biopesticides. The Health 2012; 3:116-120.
  • Awad T. I, Onder F, Kismali S. Azadirachta indica A. Juss (Meliaceae) ağacından elde edilen doğal pestisitler üzerinde bir inceleme. Turk Entomol Derg 1998; 22 (3), 225- 240.
  • Bhowmik D, Chiranjib J.Y, Tripathi K.K, Kumar S. Herbal remedies of Azadirachta indica and its medicinal application. J Chem Pharm Res 2010; 2 (1), 62- 72.
  • Pankaj S, Lokeshwar T, Mukesh B, Vishnu B. Review on Neem (Azadirachta indica): thousand problems one solution. Int Res J Pharm 2011; 2 (12), 97- 102.
  • Barnby M. A, Klocke J.A. Effects of azadirachtin on the nutrition and development of the tobacco budworm, Heliothis virescens (Fabr.) (Noctuidae). J Insect Physiol 1987; l33:69.
  • Koul O. Biological activity of volatile D-n-Propyl Difulside from seeds of neem, Azadirachta indica (Meliaceae), to two species of stored grain pests, Sitophilus oryzae (L.) and Tribolium oryzae (Herbst). J Econ Entomol 2004; 97:1142-1147.
  • Dere B, Altuntaş H, Nurullahoğlu Z.U. Insecticidal and oxidative effects of azadirahtin on the model organism Galleria mellonella L. (Lepidoptera: Pyralidae). Arch Insect Biochem Physiol 2015; 89:138-52.
  • Mordue (Luntz) A.J, Nisbet A. Azadirachtin from the Neem Tree Azadirachta indica: its Action Against Insects. Ann Soc Entomol 2000; 29:615-632.
  • Malczewska M, Gelman S.B, Cymborowski B. Effect of azadirachtin on development, juvenile hormone and ecdysteroid titres in chilled Galleria mellonella larvae. J Insect Physiol 1988; 34:725-732.
  • Adel M. M, Sehnal F. Azadirachtin potentiates the action of ecdysteroid agoinst RH-2485 in Spodoptera littoralis. J Insect Physiol 2000; 46: 267-274.
  • Charriere J.D, Imdorf A. Protection of honey combs from moth damage. Liebefeld, Bern, Switzerland, 1997.
  • Rajendran S, Hajira Parveen K.M. Insect infestation in stored animal products. J Stor Product Res 2005; 41, 1–30.
  • Kwadha C.A, Ong’amo G.O, Ndegwa P.N, Raina S.K, Fombong A.T. The biology and control of the greater wax moth Galleria mellonella. Insects 2017; 8(61): 1-17.
  • Altınçiçek B, Lindner M, Linder D, Preissner K, Vilcinskas A. Microbial metalloproteinases mediate sensing of invading pathogens and activate innate immune responses in the lepidopteran model host Galleria mellonella. Infection and Immunity 2007; 75: 175-183.
  • Altuntaş H, Duman E, Şanal-Demirci S.N, Ergin E. Toxicological and physiological effects of ethephon on the model organism, Galleria mellonella L. 1758 (Lepidoptera: Pyralidae). Turk Entomol Derg 2016; 40(4), 413-423.
  • Maguire R, Duggan O, Kavanagh K. Evaluation of Galleria mellonella larvae as an in vivo model for assessing the relative toxicity of food preservative agents. Cell Biol Toxicol 2016; 32(3):209-16.
  • Bronskill J.K. A cage to simplify the rearing of greater wax moth, Galleria mellonella (Pyralidae). Journal of Lepidopterists’ Society 1961; 15, 102-104.
  • Metspalu L, Jogar K, Ploomi A, Hiiesaar K, Kivimagi I, Luik A. Effects of biopesticide Neem EC on the Mamestra brassicae L. (Lepidoptera, Noctuidae). Agronomy Research 2010; 8:465-470.
  • Erdoğan P, Toros S. Azadirachta indica A. Juss ekstraktlarının Patates böceği [Leptinotarsa decemlineata Say (Col.: Chrysomelidae)] larvalarının gelişimi üzerine etkisi. Bitki Koruma Bülteni, 2010; 50:73-88.
  • Er A, Taşkıran D, Sak O. Azadirachtin-induced effects on various life history traits and cellular immune reactions of Galleria mellonella (Lepidoptera: Pyralidae). Arch of Biolog Scien 2017; 69(2): 335-344.
  • Simmonds M.S, Jarvis A.P, Johnson S, Jones G.R, Morgan E.D. Comparison of anti-feedant and insecticidal activity of nimbin and salannin photooxidation products with neem (Azadirachta indica) limonoids. Pest Manag Scien 2004; 60, 459-464.
  • Wondafrash M, Getu E, Terefe G. Survival and feeding of African bollworm, Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) affected by Neem, Azadirachta indica (A. Juss) extracts. WJAS 2012; 8 (3): 280-285.
  • Jogar K, Kuusik A, Metspalu L, Hiiesaar K, Grishakova M, Luik A. Effects of neem EC on gas exchange, tracheal ventilation, and water loss in diapausing pupae of Pieris brassicae. The Authors Entomologia Experimentalis et Applicata, 2008; 126, 165-173.
  • Roel A.R, Dourado D.M, Matias R, Porto K.R, Bednaski A.V, Da Costa R.B. The effect of sub-lethal doses of Azadirachta indica (Meliaceae) oil on the midgut of Spodoptera frugiperda (Lepidoptera, Noctuidae). Revista Brasileira de Entomologia 2010; 54(3), 505-510.
There are 33 citations in total.

Details

Journal Section Articles
Authors

Hülya Altuntaş This is me

Beyza Dere This is me

Zahide Ulya Nurullahoğlu This is me

Publication Date January 1, 2019
Published in Issue Year 2019 Volume: 8 Issue: 1

Cite

APA Altuntaş, H., Dere, B., & Nurullahoğlu, Z. U. (2019). Azadirachtin’in model böcek Galleria mellonella (Lepidoptera: Pyralidae)’nın gelişimi üzerine etkileri. Eskişehir Teknik Üniversitesi Bilim Ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji, 8(1), 85-91.
AMA Altuntaş H, Dere B, Nurullahoğlu ZU. Azadirachtin’in model böcek Galleria mellonella (Lepidoptera: Pyralidae)’nın gelişimi üzerine etkileri. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji. January 2019;8(1):85-91.
Chicago Altuntaş, Hülya, Beyza Dere, and Zahide Ulya Nurullahoğlu. “Azadirachtin’in Model böcek Galleria Mellonella (Lepidoptera: Pyralidae)’nın gelişimi üzerine Etkileri”. Eskişehir Teknik Üniversitesi Bilim Ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 8, no. 1 (January 2019): 85-91.
EndNote Altuntaş H, Dere B, Nurullahoğlu ZU (January 1, 2019) Azadirachtin’in model böcek Galleria mellonella (Lepidoptera: Pyralidae)’nın gelişimi üzerine etkileri. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 8 1 85–91.
IEEE H. Altuntaş, B. Dere, and Z. U. Nurullahoğlu, “Azadirachtin’in model böcek Galleria mellonella (Lepidoptera: Pyralidae)’nın gelişimi üzerine etkileri”, Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji, vol. 8, no. 1, pp. 85–91, 2019.
ISNAD Altuntaş, Hülya et al. “Azadirachtin’in Model böcek Galleria Mellonella (Lepidoptera: Pyralidae)’nın gelişimi üzerine Etkileri”. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 8/1 (January 2019), 85-91.
JAMA Altuntaş H, Dere B, Nurullahoğlu ZU. Azadirachtin’in model böcek Galleria mellonella (Lepidoptera: Pyralidae)’nın gelişimi üzerine etkileri. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji. 2019;8:85–91.
MLA Altuntaş, Hülya et al. “Azadirachtin’in Model böcek Galleria Mellonella (Lepidoptera: Pyralidae)’nın gelişimi üzerine Etkileri”. Eskişehir Teknik Üniversitesi Bilim Ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji, vol. 8, no. 1, 2019, pp. 85-91.
Vancouver Altuntaş H, Dere B, Nurullahoğlu ZU. Azadirachtin’in model böcek Galleria mellonella (Lepidoptera: Pyralidae)’nın gelişimi üzerine etkileri. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji. 2019;8(1):85-91.