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SIMULTANEOUS QUANTITATIVE ANALYSIS OF TWO ANTHELMINTIC DRUGS IN A VETERINARY DOSAGE FORM

Year 2022, Volume 11, Issue 1, 22 - 30, 19.01.2022
https://doi.org/10.18036/estubtdc.1051707

Abstract

In this research article, a new chromatographic method ultra-performance liquid chromatography (UPLC) method was developed for the quantification of ivermectin (IMT)-preziquantel (PRZQ) in a two-component mixture and veterinary dosage form mixture. The separation of the studied veterinary drugs (IMT and PRZQ) was carried out on Waters Acquity ® BEH C18 column (50mm x 2.1 mm i.d., 1.7 m) using a mobile phase consisting of water-acetonitrile-methanol (10:70:20, v/v). In the chromatographic analysis, flow rate was 0.38 mL/min, and column temperature was maintained at 42 0C. The chromatographic detection of IMT and PRZQ was made at the wavelength of 220.0 and 245.0 nm, respectively. The developed UPLC method was validated and applied to real samples consisting of IMT and PRZQ. Recovery assay results were found to be 100.4 % for PRZQ and 101.8 % for IMT. The presented UPLC method is introduced as an alternative choice for the quality control of pharmaceutical preparations containing these drugs. For the quality control of pharmaceutical preparations containing these drugs, the presented UPLC method is introduced as an alternative experimental analysis choice.

References

  • Del Guidice P, Marty P. Ivermectin: A new therapeutic weapon in dermatology? Arch Dermatol, 1999;135:705-6.
  • [2] Takayangui OM. Neurocisticercose avaliacao da terapeutica com praziquantel. [Neurocysticercosis. II. Evaluation of treatment with praziquantel. Arq Neuro-Psiquiat, 1990; 48(1): 11–15.
  • [3] Koul PA, Waheed A, Hayat M, Sofi BA. Praziquantel in niclosamide-resistant Taenia saginata infection. Scand J Infect Dis, 1999; 31(6): 603–4.
  • [4] Piantavini MS, Pontes FL, Weiss LX, Senab MM, Pontarolo R. Comparison between ultraviolet and infrared spectroscopies for the simultaneous multivariate determination of pyrantel and praziquantel. J Braz Chem Soc, 2015;26(7):1387-95. doi:10.5935/0103-5053.20150107
  • [5] Phatak HM, Vaidya VV, Phatak MS, Rajeghadge D. A rapid high performance liquid chromatography method for simultaneous quantification of praziquantel, ivermectin and abamectin from veterinary formulations: Development, validation and application. Int J Pharm Res Scholars, 2016;5(1):57-65.
  • [6] Vijay Kumar G, Sravanthi B, Praveen A. Analytical method development and validation for ivermectin and albendazole in combine dosage form by RPHPLC. Int J Curr Trends Pharma Res, 2019;7(1):7-12.
  • [7] Bhavya B, Nagaraju P, Mounika V, Priyadarshini GI. Stability indicating RP-HPLC method development and validation for simultaneous estimation of albendazole and ivermectin in pharmaceutical dosage form. Asian J Pharm Anal, 2017;7(1):6-14. doi:10.5958/2231-5675.2017.00002.3
  • [8] Rupali S, Shyamala B, Kulesh K, Shailendra KJ. Simultaneous estimation of pyrantel pamoate, praziquantel & febantel by high performance liquid chromatography using dual wavelength. J Appl Pharm Res, 2014;2(2):32-43.
  • [9] Rajesh R, Jithu JJ. A validated RP-HPLC method for simultaneous estimation of pyrantel pamoate and praziquantel in bulk and pharmaceutical dosage form. Int J Pharm Pharm Sci, 2019;11(5):62-7. doi:10.22159/ijpps.2019v11i5.30488
  • [10] Kumudini SR, Sunil RD, Vidhya KB, Amruta LS. Validated HPTLC method for simultaneous estimation of ivermectin and albendazole in formulation. Asian J Pharm Biol Res, 2011;1(3):330- 6.
  • [11] Pontes FL, Pontarolo RO, Campos FR, Gasparetto JC, Cardoso MA, Piantavini MS, et al. Development and validation of an HPLC-MS/MS method for simultan ous determination of ivermectin, febantel, praziquantel, pyrantel pamoate and related compounds in fixed dose combination for veterinary use. Asian J Pharm Clin Res, 2013;6(2):191-9.
  • [12] Gandla K, Lalitha R, Varun D, Shruthi P, TejaVR Analytical Method Validation of Ivermectin and Praziquantel in Bulk and Pharmaceutical Dosage Form by UPLC, International Journal of Research Publication and Reviews, 2021; 2 (4):573-583.

VETERİNER DOZAJ FORMUNDA İKİ ANTELMİNTİK İLACIN EŞZAMANLI KANTİTATİF ANALİZİ

Year 2022, Volume 11, Issue 1, 22 - 30, 19.01.2022
https://doi.org/10.18036/estubtdc.1051707

Abstract

Bu araştırma makalesinde, iki bileşenli bir karışım ve veteriner dozaj formu karışımında ivermektin (IMT)-prezikuantel (PRZQ) miktarının belirlenmesi için yeni bir kromatografik yöntem ultra-performans sıvı kromatografisi (UPLC) yöntemi geliştirilmiştir. İncelenen veteriner ilaçlarının (IMT ve PRZQ) ayrılması, su-asetonitril-metanolden (10:70:20, v/v). Kromatografik analizde akış hızı 0.38 mL/dk idi ve kolon sıcaklığı 42 0C'de tutuldu. IMT ve PRZQ'nun kromatografik tespiti sırasıyla 220.0 ve 245.0 nm dalga boyunda yapıldı. Geliştirilen UPLC yöntemi valide edilmiş ve IMT ve PRZQ'dan oluşan gerçek örneklere uygulanmıştır. Geri kazanım tahlil sonuçlarının PRZQ için %100.4 ve IMT için %101.8 olduğu bulundu. Sunulan UPLC yöntemi, bu ilaçları içeren farmasötik preparasyonların kalite kontrolü için alternatif bir seçenek olarak sunulmuştur. Bu ilaçları içeren farmasötik preparatların kalite kontrolü için, alternatif bir deneysel analiz seçeneği olarak sunulan UPLC yöntemi tanıtılmıştır.

References

  • Del Guidice P, Marty P. Ivermectin: A new therapeutic weapon in dermatology? Arch Dermatol, 1999;135:705-6.
  • [2] Takayangui OM. Neurocisticercose avaliacao da terapeutica com praziquantel. [Neurocysticercosis. II. Evaluation of treatment with praziquantel. Arq Neuro-Psiquiat, 1990; 48(1): 11–15.
  • [3] Koul PA, Waheed A, Hayat M, Sofi BA. Praziquantel in niclosamide-resistant Taenia saginata infection. Scand J Infect Dis, 1999; 31(6): 603–4.
  • [4] Piantavini MS, Pontes FL, Weiss LX, Senab MM, Pontarolo R. Comparison between ultraviolet and infrared spectroscopies for the simultaneous multivariate determination of pyrantel and praziquantel. J Braz Chem Soc, 2015;26(7):1387-95. doi:10.5935/0103-5053.20150107
  • [5] Phatak HM, Vaidya VV, Phatak MS, Rajeghadge D. A rapid high performance liquid chromatography method for simultaneous quantification of praziquantel, ivermectin and abamectin from veterinary formulations: Development, validation and application. Int J Pharm Res Scholars, 2016;5(1):57-65.
  • [6] Vijay Kumar G, Sravanthi B, Praveen A. Analytical method development and validation for ivermectin and albendazole in combine dosage form by RPHPLC. Int J Curr Trends Pharma Res, 2019;7(1):7-12.
  • [7] Bhavya B, Nagaraju P, Mounika V, Priyadarshini GI. Stability indicating RP-HPLC method development and validation for simultaneous estimation of albendazole and ivermectin in pharmaceutical dosage form. Asian J Pharm Anal, 2017;7(1):6-14. doi:10.5958/2231-5675.2017.00002.3
  • [8] Rupali S, Shyamala B, Kulesh K, Shailendra KJ. Simultaneous estimation of pyrantel pamoate, praziquantel & febantel by high performance liquid chromatography using dual wavelength. J Appl Pharm Res, 2014;2(2):32-43.
  • [9] Rajesh R, Jithu JJ. A validated RP-HPLC method for simultaneous estimation of pyrantel pamoate and praziquantel in bulk and pharmaceutical dosage form. Int J Pharm Pharm Sci, 2019;11(5):62-7. doi:10.22159/ijpps.2019v11i5.30488
  • [10] Kumudini SR, Sunil RD, Vidhya KB, Amruta LS. Validated HPTLC method for simultaneous estimation of ivermectin and albendazole in formulation. Asian J Pharm Biol Res, 2011;1(3):330- 6.
  • [11] Pontes FL, Pontarolo RO, Campos FR, Gasparetto JC, Cardoso MA, Piantavini MS, et al. Development and validation of an HPLC-MS/MS method for simultan ous determination of ivermectin, febantel, praziquantel, pyrantel pamoate and related compounds in fixed dose combination for veterinary use. Asian J Pharm Clin Res, 2013;6(2):191-9.
  • [12] Gandla K, Lalitha R, Varun D, Shruthi P, TejaVR Analytical Method Validation of Ivermectin and Praziquantel in Bulk and Pharmaceutical Dosage Form by UPLC, International Journal of Research Publication and Reviews, 2021; 2 (4):573-583.

Details

Primary Language English
Subjects Biotechnology and Applied Microbiology
Journal Section Articles
Authors

Eda BÜKER (Primary Author)
gazi üniversitesi
0000-0002-9427-3119
Türkiye


Nagihan YAŞAR
ANKARA UNIVERSITY
0000-0002-1770-6013
Türkiye


Erdal DİNÇ
ANKARA UNIVERSITY
0000-0001-6326-1441
Türkiye

Publication Date January 19, 2022
Published in Issue Year 2022, Volume 11, Issue 1

Cite

Bibtex @research article { estubtdc1051707, journal = {Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji}, issn = {2667-4203}, address = {btdc@anadolu.edu.tr}, publisher = {Eskisehir Technical University}, year = {2022}, volume = {11}, number = {1}, pages = {22 - 30}, doi = {10.18036/estubtdc.1051707}, title = {SIMULTANEOUS QUANTITATIVE ANALYSIS OF TWO ANTHELMINTIC DRUGS IN A VETERINARY DOSAGE FORM}, key = {cite}, author = {Büker, Eda and Yaşar, Nagihan and Dinç, Erdal} }
APA Büker, E. , Yaşar, N. & Dinç, E. (2022). SIMULTANEOUS QUANTITATIVE ANALYSIS OF TWO ANTHELMINTIC DRUGS IN A VETERINARY DOSAGE FORM . Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji , 11 (1) , 22-30 . DOI: 10.18036/estubtdc.1051707
MLA Büker, E. , Yaşar, N. , Dinç, E. "SIMULTANEOUS QUANTITATIVE ANALYSIS OF TWO ANTHELMINTIC DRUGS IN A VETERINARY DOSAGE FORM" . Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 11 (2022 ): 22-30 <https://dergipark.org.tr/en/pub/estubtdc/issue/67947/1051707>
Chicago Büker, E. , Yaşar, N. , Dinç, E. "SIMULTANEOUS QUANTITATIVE ANALYSIS OF TWO ANTHELMINTIC DRUGS IN A VETERINARY DOSAGE FORM". Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 11 (2022 ): 22-30
RIS TY - JOUR T1 - SIMULTANEOUS QUANTITATIVE ANALYSIS OF TWO ANTHELMINTIC DRUGS IN A VETERINARY DOSAGE FORM AU - Eda Büker , Nagihan Yaşar , Erdal Dinç Y1 - 2022 PY - 2022 N1 - doi: 10.18036/estubtdc.1051707 DO - 10.18036/estubtdc.1051707 T2 - Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji JF - Journal JO - JOR SP - 22 EP - 30 VL - 11 IS - 1 SN - 2667-4203- M3 - doi: 10.18036/estubtdc.1051707 UR - https://doi.org/10.18036/estubtdc.1051707 Y2 - 2022 ER -
EndNote %0 Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji SIMULTANEOUS QUANTITATIVE ANALYSIS OF TWO ANTHELMINTIC DRUGS IN A VETERINARY DOSAGE FORM %A Eda Büker , Nagihan Yaşar , Erdal Dinç %T SIMULTANEOUS QUANTITATIVE ANALYSIS OF TWO ANTHELMINTIC DRUGS IN A VETERINARY DOSAGE FORM %D 2022 %J Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji %P 2667-4203- %V 11 %N 1 %R doi: 10.18036/estubtdc.1051707 %U 10.18036/estubtdc.1051707
ISNAD Büker, Eda , Yaşar, Nagihan , Dinç, Erdal . "SIMULTANEOUS QUANTITATIVE ANALYSIS OF TWO ANTHELMINTIC DRUGS IN A VETERINARY DOSAGE FORM". Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 11 / 1 (January 2022): 22-30 . https://doi.org/10.18036/estubtdc.1051707
AMA Büker E. , Yaşar N. , Dinç E. SIMULTANEOUS QUANTITATIVE ANALYSIS OF TWO ANTHELMINTIC DRUGS IN A VETERINARY DOSAGE FORM. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji. 2022; 11(1): 22-30.
Vancouver Büker E. , Yaşar N. , Dinç E. SIMULTANEOUS QUANTITATIVE ANALYSIS OF TWO ANTHELMINTIC DRUGS IN A VETERINARY DOSAGE FORM. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji. 2022; 11(1): 22-30.
IEEE E. Büker , N. Yaşar and E. Dinç , "SIMULTANEOUS QUANTITATIVE ANALYSIS OF TWO ANTHELMINTIC DRUGS IN A VETERINARY DOSAGE FORM", Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji, vol. 11, no. 1, pp. 22-30, Jan. 2022, doi:10.18036/estubtdc.1051707