Amitriptilinin Çipura (Sparus aurata) Serum Biyokimyası Üzerindeki Etkisi

Öz

Amitriptilin, depresyonun yanında, birçok nöropatik ve infalamatuar hastalığın tedavisinde sıklıkla kullanılan trisiklik antidepresandır. Bu çalışmada, sucul ortama karışması sonucunda uzun süre kalıcılık gösterme özelliğine sahip olan amitriptilin antidepresanının çipura (Sparus aurata) balıklarının serum biyokimyasına olan etkisi araştırılmıştır. Bu amaçla çipura balıkları 14 gün boyunca 0,2 µg/L, 10 µg/L, 100 µg/L ve 1 mg/L’lik amitriptilin konsantrasyonlarına maruz bırakılmıştır. Çalışmanın 7. ve 14. gününde alınan balıkların kan serum örneklerinden glukoz (GLU), albumin (ALB), globulin (GLO), total protein (TPROT), trigliserit (TRİ), kolesterol (KOL), aspartat aminotransferaz (AST), alanin aminotransferaz (ALT), alkalen fosfataz (ALP) ve laktat dehidrogenaz (LDH) biyokimyasal parametreleri belirlenmiştir. 7. günde anlamlı bir farklılık göstermeyen tüm serum biyokimya parametrelerinden, trigliserid, kolesterol, AST, ALP ve LDH değerlerinde 14. günde anlamlı değişimler meydana gelmiştir (p<0,05). Bu sonuçlar farklı amitriptilin konsantrasyonlarının balık biyokimyasında önemli değişimlere neden olduğunu ortaya koymaktadır.

Anahtar Kelimeler

• Sparus aurata
• amitriptilin
• serum biyokimyası

Effect of Amitriptyline on Serum Biochemistry of Gilthead Sea Bream (Sparus aurata)

Abstract

Amitriptyline is a tricyclic antidepressant that is frequently used in the treatment of many neuropathic and inflammatory diseases as well as depression. In this study, the effect of amitriptyline antidepressant, which has long-lasting properties as a result of contamination to the aquatic environment, on the serum biochemistry of sea bream (Sparus aurata) fish was investigated. For this purpose, sea bream were exposed to amitriptyline concentrations of 0.2 µg/L, 10 µg/L, 100 µg/L and 1 mg/L for 14 days. Glucose (GLU), albumin (ALB), globulin (GLO), total protein (TPROT), triglyceride (TRI), cholesterol (COL), aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) biochemical parameters were determined from the blood serum samples of the fish taken on the 7th and 14th days of the study. While all serum biochemistry parameters did not show a significant difference on the 7th day, significant changes occurred in the triglyceride, cholesterol, AST, ALP and LDH values on the 14th day (p<0.05). These results reveal that different amitriptyline concentrations cause significant changes in fish biochemistry.

Keywords

• Sparus aurata
• amitriptyline
• serum biochemistry

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