Tuzluluğun Bazı Kanola Çeşitlerinde Çimlenme ve Fide Büyümesi Üzerine Etkileri

Öz

Abstract The present thesis focused on determination of the effect of salinity on germination and seedling growth of some canola varieties. Factorial experiments were conducted in randomized complete block design with replications including 4 canola cultivars (PR44W29, NK LINUS, DK EXTORM, ES NEPTUNE) and 6 salinity levels (0, 5, 10, 15, 20, 25% EC generated through addition of 0, 2.75, 5.72, 9, 12.45 and 15.70 g/l NaCl, respectively). Following 7 day germination tests, number of germinated seeds were counted and length of whole plant, shoot and root fresh and dry weights were measured. The highest germination rate (85.5%) was obtained from 0% EC salinity level of DK EXTORM and the lowest (1.5%) from 25% EC level of NK LINUS variety. The differences in germination rates of the experimental groups were not found to be significant (P>0.05). The highest seedling height (74 mm) was obtained from 5% EC level of NK LINUS variety and the lowest from 25% EC level of PR44W29 and DK EXTORM varieties (P<0.05). The highest root length (64.1 mm) was observed in 5% EC level of NK LINUS variety. However, because of high salinity levels, ES Neptune and DK EXTORM and PR44W29 varieties did not achieve root development at 25% EC salinity level (P<0.05). The highest fresh weight (125.9 g) was obtained from 5% EC level of NK LINUS variety and the lowest (0 g, all seedlings were dead) from 25% EC level of ES Neptune and DK EXTORM varieties (P<0.05). The highest dry weight (5.5 g) was obtained from 20% EC level of NK LINUS variety. Dry weights were not able to be measured in ES Neptune and DK EXTORM varieties since seeds died at high level salinity levels. The lowest dry weights (2.1 and 2.7 g) were obtained from 25% EC level of DK EXTORM PR44W29 varieties, respectively. In general, decreasing germination, seedling length, fresh and dry weights were observed with increasing salinity levels (from 0% EC to 25% EC).

Anahtar Kelimeler

• canola
• salinity
• germination
• seedling development

The Effects of Salinity on Germination and Seedling Growth of Some Canola Varieties

Abstract

Abstract The present thesis focused on determination of the effect of salinity on germination and seedling growth of some canola varieties. Factorial experiments were conducted in randomized complete block design with replications including 4 canola cultivars (PR44W29, NK LINUS, DK EXTORM, ES NEPTUNE) and 6 salinity levels (0, 5, 10, 15, 20, 25% EC generated through addition of 0, 2.75, 5.72, 9, 12.45 and 15.70 g/l NaCl, respectively). Following 7 day germination tests, number of germinated seeds were counted and length of whole plant, shoot and root fresh and dry weights were measured. The highest germination rate (85.5%) was obtained from 0% EC salinity level of DK EXTORM and the lowest (1.5%) from 25% EC level of NK LINUS variety. The differences in germination rates of the experimental groups were not found to be significant (P>0.05). The highest seedling height (74 mm) was obtained from 5% EC level of NK LINUS variety and the lowest from 25% EC level of PR44W29 and DK EXTORM varieties (P<0.05). The highest root length (64.1 mm) was observed in 5% EC level of NK LINUS variety. However, because of high salinity levels, ES Neptune and DK EXTORM and PR44W29 varieties did not achieve root development at 25% EC salinity level (P<0.05). The highest fresh weight (125.9 g) was obtained from 5% EC level of NK LINUS variety and the lowest (0 g, all seedlings were dead) from 25% EC level of ES Neptune and DK EXTORM varieties (P<0.05). The highest dry weight (5.5 g) was obtained from 20% EC level of NK LINUS variety. Dry weights were not able to be measured in ES Neptune and DK EXTORM varieties since seeds died at high level salinity levels. The lowest dry weights (2.1 and 2.7 g) were obtained from 25% EC level of DK EXTORM PR44W29 varieties, respectively. In general, decreasing germination, seedling length, fresh and dry weights were observed with increasing salinity levels (from 0% EC to 25% EC).

Keywords

• canola
• salinity
• germination
• seedling development

References

1. J. Bushong, J. Lofton, H. Sanders, and M. Stamm, “Great Plains Canola Production Handbook”, Kansas State University, Agricultural Experiment Station and Cooperative Extension Service, MF2734. Kansas State University, 2018.
2. S. Channaoui, R. E. Kahkahi, J. Charafi, H. E. Mazouz, and A. Nabloussi, “Germination and Seedling Growth of a Set of Rapeseed (Brassica napus) Varieties under Drought Stress Conditions”, Int. J. Environ. Agric. Biotechnol, (IJEAB), vol. 2, pp. 487–494, 2017.
3. L. Rajjou, M. Duval, K. Gallardo, J. Catusse, J. Bally, C. Job, and D. Job, “Seed germination and vigor”, Annu. Rev. Plant Biol., vol. 63, pp. 507–533, 2012.
4. N. Puppala, J.L. Fowler, L. Poindexter, and H.L. Bhardwaj, “Evaluation of salinity tolerance of canola germination”, in J. Janick, ed. Perspectives on new crops and new uses. ASHS Press, Alexandria, VA, pp. 251–253, 1999.
5. S. Gyawali, I.A.P. Parkin, H. Steppuhn, M. Buchwaldt, B. Adhikari, R. Wood, K. Wall, L. Buchwaldt, M. Singh, D. Bekkaoui, and D.D. Hegedus “Seedling, early vegetative, and adult plant growth of oilseed rapes (Brassica napus L.) under saline stress”, Canadian Journal of Plant Science, vol. 99, no. 6, pp. 921–947, 2019. M. Qasim, M. Ashraf, M.Y. Ashraf, S.U. Rehman, and E.S. Rha, “Salt-induced changes in two canola cultivars differing in salt tolerance”, Biol. Plant. vol. 46, no. 4, pp. 629–632 2003.
6. W. J. Katembe, I. A. Ungar and J. P. Mıtchell, “Effect of Salinity on Germination and Seedling Growth of two Atriplex species (Chenopodiaceae)”, Annals of Botany, vol. 82, no. 2, pp. 167-175, 1998.
7. A.A Kandil, A.E. Sharief, W.A.E. Abido and M.M.O. Ibrahim, “Response of some canola cultivars (Brassica napus L.) to salinity stress and its effect on germination and seedling properties”, J. Crop Sci. vol. 3, pp. 95–103, 2012.
8. A. Bybordi, S. Tabatabaei and A. Ahmedov, “Effect of salinity on the growth and peroxidase and IAA oxidase activities in canola, Journal of Food Agriculture and Environment” vol. 8, no. 1, pp. 109–112, 2010.

9. İ. Atiş, “Effects of Salt Stress on Germination and Seedling Development of Some Silage Sorghum (Sorghum bicolor L. Moench) Cultivars”, SDÜ Ziraat Fakültesi Dergi., vol. 6, no. 2, pp. 58–67, 2011.
10. E. Shahbazi, A. Arzani, and G. Saeidi, “Effects of NaCl treatments on seed germination and antioxidant activity of canola (Brassica napus L.) cultivars”, Bangladesh J. Bot. vol. 41, no. 1, pp. 67–73, 2011.
11. M. Arslan, B. Aydınoğlu, “Effect of salinity (NaCl) stress on germination and early seedling growth characteristics of damson (Lathyrus sativus L.)”. Akad. Ziraat Derg. 49–54. 2018.
12. M. Jamil, C.C. Lee, S. Rehman, D.B. Lee, M. Ashraf and E.S. Rha, “Salinity (NaCl) tolerance of brassica species at germination and early seedling growth”. J. Environ. Agric. Food Chem., 4: 970-976, 2005.
13. J. Huang, R.E. Redmann, “Salt tolerance of Hordeum and Brassica species during germination and early seedling growth”, Can. J. Plant Sci. 75: 815–819, 1995.
14. S. Gulzar, M.A. Khan and I.A. Ungar, “Effect of Salinity and Temperature on the Germination of Urochongra setulosa (Trin.) C.E. Hubbard”, Seed Sci. Technol., 29:21-29, 2001.
15. M. Tester and R. Davenport, “Na+ tolerance and Na+ transport in higher plants”, Ann. Bot. 91:503-527, 2003.
16. Jamil, M., K.B. Lee, K.Y. Jung, D.B. Lee, M.S. Han and E.S. Rha. 2007. Salt stress inhibits germination and early seedling growth in cabbage (Brassica oleracea capitata L.). Pak. J. Biol. Sci., 10(6): 910-914.
17. A. Baybordi, “The influence of salt stress on seed germination, growth and yield of canola cultivars, Not. Bot. Hort. Agrobot. Cluj., 38(1): 128-133, 2010.
18. F. Kayacetin, “Selection of some important species in genus Brassica against drought and salt tolerance by morphological observations on germination and seedling growth parameters”. Fresenius Environmental Bulletin, 30(1): 60–69, 2021.
19. F. Kayaçetin, “Correlation among germination and seedling parameters of Brassica juncea under PEG 6000 and NaCl treatments”, Int J Agric For Life Sci, 6(1): 8-11, 2022.
20. J.K. Zhu, “Salt and Drought Stress Signal Transduction in Plants”. Annual Review of Plant Biology, 53, 247-273, 2002.