Cytotoxic Effects of Biosynthesized zinc nanoparticles on Normal fibroblast cells and their antimicrobial effect on pathogenic strains

Öz

Nanoparticles, particularly zinc nanoparticles (ZnNPs), have garnered substantial interest in biomedical research for their potential applications in therapeutics and antimicrobial therapy. In this study, we investigated the cytotoxic effects of biosynthesized zinc nanoparticles on normal fibroblast cells and evaluated their antimicrobial efficacy against pathogenic strains. The biosynthesis of zinc nanoparticles was achieved through an eco-friendly and cost-effective approach utilizing biological agents. The cytotoxicity of biosynthesized zinc nanoparticles was assessed on normal fibroblast cells, serving as a model for evaluating biocompatibility. Our results indicate that biosynthesized zinc nanoparticles exhibited minimal cytotoxicity towards normal fibroblast cells, suggesting their potential safety for biomedical applications. Furthermore, the antimicrobial activity of biosynthesized zinc nanoparticles was evaluated against pathogenic strains, including antibiotic-resistant bacteria. The antimicrobial efficacy of zinc nanoparticles demonstrated promising results, highlighting their potential as alternative antimicrobial agents. Overall, this study provides valuable insights into the cytotoxic effects of biosynthesized zinc nanoparticles on normal fibroblast cells and underscores their antimicrobial potential against pathogenic strains, offering opportunities for the development of nanoparticle-based therapeutics and antimicrobial agents in medicine and healthcare.

Anahtar Kelimeler

• Antimicrobial activity
• MTT cell viability assay
• Scanning Electron Microscopy
• Zinc nanoparticles.

References

1. R. Gupta, H. Xie, K. Nan, et al., “Zinc nanoparticles as a potential agent for the management of acute respiratory distress syndrome,” Drug Delivery and Translational Research, Vol.10, no. 4, pp. 891-899, 2020.
2. Y. Chen, L. Zhang, L. Gao, et al. “Fibroblast cells play a pivotal role in tissue repair and maintenance, making them an important model for assessing the biocompatibility of nanoparticles,” Nanomedicine, Vol. 16, no. 19, pp. 1577-1590, 2021.
3. M.S. Mirtaleb, M. Hosseini, A. Tavakoli, et al., “Moreover, the antimicrobial properties of zinc nanoparticles have drawn attention as a promising strategy to combat microbial infections,” Journal of Global Antimicrobial Resistance, Vol. 27, pp. 243-255, 2023.
4. R. Sharmila and R. Gayathri, “Green Synthesis of Zinc Oxide Nanoparticles by using Hibiscus rosa- sinensis,” International Journal of Current Engineering and Technology, Vol. 4, no. 4, pp. 2444-2446, 2014.
5. M. Labieniec and T. Gabryelak, “Effects of tannins on Chinese hamster cell line B14,” Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Vol. 539, pp. 127-135, 2003.
6. E. Lapshina, I. Zavodnik and M. Labieniec, “Cytotoxic and genotoxic effects of tert-butyl hydroperoxide on Chinese hamster B14 cells,” Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Vol. 583, pp. 189-197, 2005.
7. M. G. Mayuri, P. A. Mane, R. P. Gambhir, V. S. Parkhe, P. A. Kamble, C. D. Lokhande & A. P. Tiwari, "Green synthesis of silver nanoparticles via Aloe barbadensis miller leaves: Anticancer, antioxidative, antimicrobial and photocatalytic properties." Applied Surface Science Advances, Vol. 16, pp. 100426, 2023.
8. O. Solabomi, A. Yasmine, Z. Muchen, F. Hatem, H. Xianxian, I. Ezzeldin , I. Md. Mahidul, H. Afsana, M. Jianchu and Li. Bin, “Green synthesis of zinc oxide nanoparticles using different plant extracts and their antibacterial activity against Xanthomonas oryzae pv. oryzae,” Artificial Cells, Nanomedicine and Biotechnology, Vol. 47, no. 1, pp. 341–352, 2019.

9. M. Samira, G. Mahmood, R. Mohammad and A. Mohsen, “Synthesis and optical characterization of copper nanoparticles prepared by laser ablation,” Bulletin of Materials Science, Vol.40, no. 1, pp. 37-43, 2017.
10. H. Tarek, M. Andrzej, D. Brian, Y. Amy, D. Jon and R. James, “A novel surfactant nanoemulsion with a unique non-irritant topical antimicrobial activity against bacteria, enveloped viruses and fungi,” Microbiological Research, Vol. 156, pp. 1–7, 2001.
11. A. A. Alyamani, S. Albukhaty, S. Aloufi, F.A. AlMalki, H. Al-Karagoly, G.M. Sulaiman, “Green fabrication of Zinc Oxide Na-noparticles Using Phlomis Leaf Extract: Characterization and in Vitro Evaluation of Cytotoxicity and Anti-bacterial Properties,” Molecules, Vol. 26, pp. 6140, 2021.
12. Sharmila, M. Thirumarimurugan & C. Muthukumaran, "Green synthesis of ZnO nanoparticles using Tecoma castanifolia leaf extract: Characterization and evaluation of its antioxidant, bactericidal and anticancer activities." Microchemical Journal, Vol. 145, pp.578-587, 2019.
13. G.Supriya, S. Chaitanya kumari, “Green synthesis of silver nanoparticles using Aloe vera extract and assessing their antimicrobial activity against skin infections,” International Journal of Scientific Research in Biological Sciences, Vol.6, Special Issue.1, pp.60-65, 2019.
14. Kumar, A. Choudhary, H. Kaur, S. Guha, S. Mehta & A. Husen, "Potential applications of engineered nanoparticles in plant disease management: a critical update." Chemosphere, Vol. 295, pp. 133798, 2022.
15. Ahmad, & D. Kalra, "Green synthesis, characterization and antimicrobial activities of ZnO nanoparticles using Euphorbia hirta leaf extract." Journal of King Saud University - Science, Vol. 32, Issue. 4, pp. 2358–2364, 2020.
16. Rambabu, G. Bharath, F. Banat & P. L. Show, "Green synthesis of zinc oxide nanoparticles using Phoenix dactylifera waste as bioreductant for effective dye degradation and antibacterial performance in wastewater treatment." Journal of Hazardous Materials, Vol. 402, pp. 123560, 2021.
17. K. He, Y. Liu, A. Mustapha & M. Lin, "Antifungal activity of zinc oxide nanoparticles against Botrytis cinerea and Penicillium expansum." Microbiological Research, Vol. 166, Issue. 3, pp. 207–215, 2011.
18. T. U. D. Thi, T. T. Nguyen, Y. D. Thi, K. H. T. Thi, B. T. Phan & K. N. Pham, "Green synthesis of ZnO nanoparticles using orange fruit peel extract for antibacterial activities." RSC Advances, Vol. 10, Issue. 40, pp. 23899–23907, 2020.
19. Umavathi, S. Mahboob, M. Govindarajan, K. A. Al-Ghanim, Z. Ahmed, et al., "Green synthesis of ZnO nanoparticles for antimicrobial and vegetative growth applications: a novel approach for advancing efficient high quality health care to human wellbeing." Saudi Journal of Biological Sciences, Vol. 28, Issue. 3, pp. 1808–1815, 2021.
20. A. M. Pillai, V. S. Sivasankarapillai, A. Rahdar, J. Joseph, F. Sadeghfar, et al., "Green synthesis and characterization of zinc oxide nanoparticles with antibacterial and antifungal activity." Journal of Molecular Structure, Vol. 1211, pp. 128107, 2020.
21. P. Chen, H. Wang, M. He, B. Chen, B. Yang & B. Hu, "Size-dependent cytotoxicity study of ZnO nanoparticles in HepG2 cells." Ecotoxicology and Environmental Safety, Vol. 171, pp. 337-346, 2019.