Triklosanın Zebra Balığı (Danio rerio) İnce Bağırsağı Üzerinde Histopatolojik Etkileri

Year 2022, Volume: 2 Issue: 2, 98 - 106, 30.09.2022

Cansu Akbulut

Abstract

Bir bisfenol çeşidi olan triklosan, antibakteriyel sabun, deodorant, diş macunu, el kremi gibi kişisel bakım ürünlerinin yanı sıra kumaşlarda, oyuncak, diş fırçası sapı, mutfakta kullanılan plastikler gibi pek çok ürünün yapısında koruyucu, antiseptik, antimikrobiyal ve dezenfektan olarak kullanılmaktadır. Önemli biyositlerden ve çevresel kirleticilerden biri olan triklosan, yer altı suları aracılığı ile ekosisteme karışmakta ve sucul canlıların yaşamını tehdit etmektedir. Bu çalışma kapsamında triklosanın zebra balığı ince bağırsak dokularında yarattığı histopatolojik etkilerin incelenmesi amaçlanmıştır. Balıklara, 5 gün boyunca 34, 85 and 170 μg/L’lik triklosan uygulaması yapılmıştır. 5 günlük maruziyet süresinin sonunda ince bağırsak dokuları disekte edilmiştir. Dokular Bouin fiksatifi ile 24 saat boyunca fikse edilmiş, ardından rutin histolojik teknikler uygulanmıştır. Dokulardan mikrotom yardımı ile 5 µm kalınlığında kesitler alındıktan sonra Hematoksilen&Eozin boyaması yapılmıştır. Boyanan kesitler ışık mikroskobu altında değerlendirilerek histopatolojik değişimler gözlenmiştir. Kontrol grubunda normal ince bağırsak dokusu gözlenirken deney gruplarında villus yapısında dejenerasyon, villuslarda birleşme, inflamasyona bağlı olarak enterosit ve lenfosit sayısında artış, Goblet hücrelerinde hiperplazi ve muscularis externada kalınlaşma tespit edilmiştir.

Keywords

triklosan, ince bağırsak, zebra balığı, histopatoloji

Effects of Triclosan on Zebrafish (Danio rerio) Intestinal Histopathology

Abstract

Triclosan, a type of bisphenol, is used as a preservative, antiseptic, antimicrobial and disinfectant in the structure of many products such as antibacterial soap, deodorant, toothpaste, hand cream, as well as personal care products such as fabrics, toys, toothbrush handles, and plastics used in the kitchen. Triclosan, which is one of the important biocides and environmental pollutants, mixes with the ecosystem through groundwater and threatens the life of aquatic organisms. In this study, it was aimed to examine the histopathological effects of triclosan on zebrafish intetinal tissues. Fish were treated with 34, 85 and 170 μg/L triclosan for 5 days. At the end of the 5-days of exposure period, the intestinal tissues were dissected. Tissues were fixed with Bouin's fixative for 24 hours, followed by routine histological techniques. Hematoxylin & Eosin staining was performed after 5 µm thick sections were taken from the tissues with a microtome. Stained sections were evaluated under a light microscope and histopathological changes were observed. While normal intestinal tissue was observed in the control group, degeneration in the villus structure, fusion of the villi, an increase in the number of enterocytes and lymphocytes due to inflammation, hyperplasia in the goblet cells and thickening of the muscularis externa were detected in the experimental groups.

Keywords

triclosan, intestine, zebrafish, histopathology

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