Investigation of Behavioural Toxicity of Alpha-cypermethrin in Drosophila melanogaster

Year 2024, Volume: 4 Issue: 1, 26 - 37, 03.05.2024

Serap Kocaoğlu Cenkci Selda Öz

https://doi.org/10.59838/etoxec.1448491

Abstract

Pyrethroids are preferred insecticides due to their low toxic effect potential. Alpha-cypermethrin is an insecticide belonging to type 2 pyrethroid group which is widely used in agriculture, public and animal health applications. In this study, the effect of alpha-cypermethrin on behavioural toxicity in Drosophila melanogaster was investigated. The behavioural toxicity of alpha-cypermethrin applied at doses of 0,01; 0,05; 0,1 and 0,3 ppm to 724 hours old larvae developing from the eggs of Drosophila Oregon R+ line individuals was evaluated by pupation and pupal emergence success, adult individual mass measurement and negative geotaxis experiments. The results obtained showed that sublethal doses of alpha-cypermethrin did not cause any significant change in pupation and pupal emergence success of larvae and mass of adult individuals. The results obtained from the negative geotaxis experiment showed that alpha-cypermethrin caused a statistically significant decrease in the flight ability of adult individuals at all doses applied.

Keywords

Alpha-cypermethrin, Drosophila melanogaster, insecticide, neurotoxicity

Alfa-sipermetrin’in Davranışsal Toksisitesinin Drosophila melanogaster’de İncelenmesi

Abstract

Piretroidler toksik etki potansiyellerinin düşük olması nedeni ile kullanımı tercih edilen insektisitlerdir. Alfa-sipermetrin tarım, halk ve hayvan sağlığı uygulamalarında yaygın olarak kullanılan tip 2 piretroid grubuna ait bir insektisittir. Bu çalışmada alfa-sipermetrin’in Drosophila melanogaster’de davranışsal toksisiteye etkisi araştırılmıştır. Drosophila Oregon R+ hattına ait bireylerin yumurtalarından gelişen 724 saatlik larvalara 0,01; 0,05; 0,1 ve 0,3 ppm dozlarında uygulanan alfa-sipermetrin’in pupa oluşturma ve pupadan çıkış başarısı, ergin birey kütle ölçümü ve negatif jeotaksis deneyleri ile davranışsal toksisitesi değerlendirilmiştir. Elde edilen sonuçlar alfa-sipermetrin’in subletal dozlarının larvaların pupa oluşturma ve pupadan çıkış başarısı üzerinde ve ergin bireylerin kütleleri üzerinde anlamlı değişikliğe neden olmadığını göstermiştir. Negatif jeotaksis deneyinden elde edilen sonuçlar ise alfa-sipermetrin’in uygulanan tüm dozlarda ergin bireylerin uçuş kabiliyetlerinde istatistiksel olarak anlamlı azalmaya neden olduğunu göstermiştir.

Keywords

Alfa-sipermetrin, Drosophila melanogaster, insektisit, nörotoksisite

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