Atık küçük ruminant fötuslarında Bovine Herpes Virus-1’in moleküler olarak araştırılması

Year 2015, Volume: 31 Issue: 1, 57 - 59, 01.03.2015

Murat Sevik Oguzhan Avci

Abstract

Bu çalışmada atık küçük ruminant fötus dokularında, Bovine
Herpes Virus-1 (BoHV-1) varlığının PCR tekniği kullanılarak
belirlenmesi amaçlandı. Bu amaçla İç Anadolu Bölgesi’ndeki
farklı illerden (Konya, Aksaray ve Niğde), BoHV-1 seropozitif
sığırlar ile bir arada yaşayan, her biri farklı sürülerdeki
küçük ruminantlara ait atıklardan (43 koyun ve 23 keçi) iç
organ (akciğer, karaciğer ve dalak, toplam n=198) örnekleri
toplandı. Glikoprotein D (gD) genine spesifik polimeraz zincir
reaksiyonu (PZR) kullanılarak, BoHV-1 varlığı araştırıldı.
Doku örneklerinin hiçbirinde BoHV-1 varlığı tespit edilemedi.
Elde edilen sonuçlara dayanarak, İç Anadolu Bölgesi’nde
BoHV-1’in küçük ruminant abort vakalarında rol oynamadığı
ifade edilebilir.

Keywords

Bovine Herpesvirus 1, küçük ruminant, abort, PCR

Molecular investigation of Bovine Herpesvirus Type 1 from aborted small ruminant foetuses

Abstract

The aim of this study was carried out to determine the presence
of Bovine Herpes Virus-1 (BoHV-1) in aborted small
ruminant foetus’ tissues by using Polymerase Chain Reaction
(PCR) technique. For this purpose, aborted tissue samples
(liver, lung, and spleen, totally n=198) were collected from
small ruminants (43 sheep and 23 goats), each from different
flocks and lived together with BoHV-1 seropositive cattle, in
Central Anatolia region (Konya, Aksaray, and Niğde provinces)
of Turkey. Glycoprotein D (gD) gene based PCR was used
for the detection of BoHV-1. All tissue samples were detected
as negative for BoHV-1 DNA. According to results, it can be
expressed that BoHV-1 is not play a role in abortion cases in
small ruminants in Central Anatolia Region.

Keywords

Bovine Herpesvirus 1, small ruminant, abortion, PCR

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