Determination of the effect of Corynebacterium cutis lysate treatment on the cytokine levels in sheep

Ayşe Er; Orhan Çorum; Burak Dik; Emre Bahçivan; Hatice Eser; Enver Yazar

Koyunlara Corynebacterium cutis lizatı uygulamasının serum sitokin düzeylerine etkisinin belirlenmesi

Year 2015, Volume: 31 Issue: 4, 209 - 213, 01.12.2015

Ayşe Er Orhan Çorum Burak Dik Emre Bahçivan Hatice Eser Enver Yazar

Abstract

Amaç: Mevcut araştırmanın amacı koyunlara Corynebacterium cutis lizatı uygulamasının serum sitokin düzeyleri üzerine etkisi ile birlikte hemogram ve rutin biyokimyasal parametrelere etkisini belirlemektir. Gereç ve Yöntem: Araştırmada 10 adet erkek Merinos toklu kullanıldı. Her bir hayvana önerilen dozda (8 mg, 0.4 mL) Corynebacterium cutis lizatı (Ultra-corn® Inj. Susp.) subkutan yolla bir kez uygulandı. Uygulamadan önce (0. saat, kontrol) ve sonraki 2, 4, 8, 12, 24, 48, 72 ve 96. saatlerde v. jugularisten kan örnekleri alındı. Elde edilen serumlardan tümör nekrozis faktör alfa (TNFα), interlöykin (IL)-1β, IL-6 ve IL-10 düzeyleri ELISA okuyucusunda belirlendi. Hemogram değerleri (Alyuvar, akyuvar, platelet, hematokrit, hemoglobin) kan hücresi sayım cihazında ve biyokimyasal (Kreatin kinaz-MB, alkalen fosfataz, alanin aminotransferaz, aspartat aminotransferaz, gamma glutamiltransferaz, total protein, albümin, kan üre nitrojen, kreatinin) değerler otoanalizörde ölçüldü.Bulgular: Corynebacterium cutis lizatı uygulamasının sitokin düzeylerinde dalgalanmalara neden olduğu gözlendi (P>0.05). Hemogram parametrelerinde istatistiki değişimlere neden olmazken, bazı biyokimyasal değerlerde (Alkalen fosfataz, total protein, albümin, kreatinin) dalgalanmalara neden olduğu tespit edildi (P

Keywords

Corynebacterium cutis, sitokin, koyun

Determination of the effect of Corynebacterium cutis lysate treatment on the cytokine levels in sheep

Year 2015, Volume: 31 Issue: 4, 209 - 213, 01.12.2015

Ayşe Er Orhan Çorum Burak Dik Emre Bahçivan Hatice Eser Enver Yazar

Abstract

Aim: The aim of this research was to determine the effect of Corynebacterium cutis lysate on cytokine levels including hemogram and routine biochemical parameters in sheep.Materials and Methods: In the research, 10 male yearling Merino sheep was used. Recommended dose (8 mg, 0.4 mL) of Corynebacterium cutis lysate (Ultra-corn® Inj.) was administered subcutaneously at a single dose to each animal. Before (0 hour, control) and after the treatments, blood samples were obtained from v. jugularis at 2, 4, 8, 12, 24, 48, 72 and 96 hours. Concentrations of serum Tumor necrosis factor alpha (TNFα), Interleukin (IL)-1β, IL-6 and IL-10 were determined with ELISA reader. Hemogram values (White blood cell, red blood cell, platelet, hematocrit, hemoglobin) were determined with hemocell counter, whereas biochemical values (Creatine kinase-MB, alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, gamma glutamyltransferase, total protein, albumin, blood urea nitrogen, creatinine) were analyzed with autoanalyzer.Results: Corynebacterium cutis lysate caused fluctuations on the cytokine levels (P>0.05). Hemogram parameters did not change statistically significant, while fluctuations were determined (P

Keywords

Corynebacterium cutis, cytokine, sheep

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Details

Other ID	JA46FY43NC
Journal Section	Research
Authors	Ayşe Er This is me Orhan Çorum This is me Burak Dik This is me Emre Bahçivan This is me Hatice Eser This is me Enver Yazar This is me
Publication Date	December 1, 2015
Published in Issue	Year 2015 Volume: 31 Issue: 4

Cite

APA	Er, A., Çorum, O., Dik, B., Bahçivan, E., et al. (2015). Determination of the effect of Corynebacterium cutis lysate treatment on the cytokine levels in sheep. Eurasian Journal of Veterinary Sciences, 31(4), 209-213.
AMA	Er A, Çorum O, Dik B, Bahçivan E, Eser H, Yazar E. Determination of the effect of Corynebacterium cutis lysate treatment on the cytokine levels in sheep. Eurasian J Vet Sci. December 2015;31(4):209-213.
Chicago	Er, Ayşe, Orhan Çorum, Burak Dik, Emre Bahçivan, Hatice Eser, and Enver Yazar. “Determination of the Effect of Corynebacterium Cutis Lysate Treatment on the Cytokine Levels in Sheep”. Eurasian Journal of Veterinary Sciences 31, no. 4 (December 2015): 209-13.
EndNote	Er A, Çorum O, Dik B, Bahçivan E, Eser H, Yazar E (December 1, 2015) Determination of the effect of Corynebacterium cutis lysate treatment on the cytokine levels in sheep. Eurasian Journal of Veterinary Sciences 31 4 209–213.
IEEE	A. Er, O. Çorum, B. Dik, E. Bahçivan, H. Eser, and E. Yazar, “Determination of the effect of Corynebacterium cutis lysate treatment on the cytokine levels in sheep”, Eurasian J Vet Sci, vol. 31, no. 4, pp. 209–213, 2015.
ISNAD	Er, Ayşe et al. “Determination of the Effect of Corynebacterium Cutis Lysate Treatment on the Cytokine Levels in Sheep”. Eurasian Journal of Veterinary Sciences 31/4 (December 2015), 209-213.
JAMA	Er A, Çorum O, Dik B, Bahçivan E, Eser H, Yazar E. Determination of the effect of Corynebacterium cutis lysate treatment on the cytokine levels in sheep. Eurasian J Vet Sci. 2015;31:209–213.
MLA	Er, Ayşe et al. “Determination of the Effect of Corynebacterium Cutis Lysate Treatment on the Cytokine Levels in Sheep”. Eurasian Journal of Veterinary Sciences, vol. 31, no. 4, 2015, pp. 209-13.
Vancouver	Er A, Çorum O, Dik B, Bahçivan E, Eser H, Yazar E. Determination of the effect of Corynebacterium cutis lysate treatment on the cytokine levels in sheep. Eurasian J Vet Sci. 2015;31(4):209-13.