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Herpes Simplex Virus tip 1 inokule edilen Vero hücre kültüründe antioksidan enzim aktiviteleri

Year 2015, Volume: 31 Issue: 2, 122 - 126, 01.06.2015

Abstract

Amaç: Bu çalışma Herpes Simplex Virus tip 1 (HSV-1) inokule edilen Vero hücre kültürlerinde antioksidan kapasite/oksidatif stres biomarkırlarını belirlemek amacı ile yapıldı.Gereç ve Yöntem: Vero hücre kültürüne HSV-1 inokule edilerek 21 saat boyunca (1. saate kadar 15 dk’da bir, 6. saate kadar saatte bir, 6-10. saat arası 30 dk’da bir, 10-21. saat arası saatte bir olmak üzere) hücre süpernatantları toplandı. Hücre süpernatantlarındaki süperoksit dismütaz (SOD), katalaz (CAT), glutasyon peroksidaz (GPX) enzimleri, glutasyon (GSH) ve malondialdehit (MDA) miktarı ticari ELISA kitleri ile ölçüldü. HSV-1’in meydana getirdiği sitopatojenik etki (CPE) ise invert mikroskop yardımı ile periyodik olarak değerlendirildi. Bulgular: Araştırmada HSV-1 inokulasyonunu takiben 6. saatte CPE gözlendi. SOD’un en yüksek ve en düşük düzeyi sırasıyla 17. ve 1. saatte belirlenirken, 18. saatte CAT ve 7. saatte GPX seviyeleri en düşük düzeyde tespit edildi. GSH miktarı ilk 30 dk içinde minimum seviyeye ulaştıktan sonra 10. saatte miktarında artış meydana geldi. MDA miktarında 3. saatte ani yükseliş olurken 18. saatte maksimum seviyeye ulaştığı belirlendi. Oksidatif stresin önemli belirteçleri olan SOD ve GSH düzeylerinin HSV-1’in neden olduğu CPE oluşumundan önce azaldığı tespit edildi. Öneri: Lipid peroksidasyonunun HSV-1’in patogenezinde rol alabileceği ve HSV-1 enfeksiyonu tedavisinde antioksidan uygulamalarının faydalı olabileceği ifade edilebilir.

References

  • Ağaçfidan A, 2012. Cinsel yolla bulaşan hastalıklarda laboratuvarda tanı olanakları. ANKEM Derg, 26, 189-197.
  • Ajuyah AO, Fenton TW, Hardin RT, Sim JS, 1993. Measuring lipid oxidation volatiles in meats. J Food Sci, 58, 70-273. Amorati R, Valgimigli L, 2014. Advantages and limitations of common testing methods for antioxidants. Free Radic Res, 6, 1-38.
  • Avci O, Yavru S, Dik I. Determination of lipid peroxidation biomarkers in Vero cell line inoculated with Bovine Ephemeral Fever Virus. Eurasian J Vet Sci, 2014, 30, 4, 217-221.
  • Beck MA, Handy J, Levander OA, 2000. The role of oxidative stress in viral infections. Ann N Y Acad Sci, 917, 906-912.
  • Bölükbaş C, Bölükbaş FF, Horoz M, Aslan M, Çelik H, Erel O, 2005. Increased oxidative stress associated with the severity of the liver disease in various forms of hepatitis B virus infection. BMC Infect Dis, 31, 95.
  • Brady RC, Bernstein DI, 2004. Treatment of herpes simplex virus infections. Antiviral Res, 61, 73-81.
  • Chandrasena LG, Peiris H, Kamani J, Wanigasuriya P, Jayaratne SD, Wijayasiri WA, Wijesekara GU, 2014. Antioxidants in patients with dengue viral infection. Southeast Asian J Trop Med Public Health, 45, 1015-1022.
  • Cusini M, Ghislanzoni M, 2001. The importance of diagnosing genital herpes. J Antimicrob Chemother, 47, 9-16.
  • Egan KP, Wu S, Wigdahl B, Jennings SR, 2013. Immunological control of herpes simplex virus infections. J Neurovirol, 19, 328-345.
  • Fatahzadeh M, Schwartz RA, 2007. Human herpes simplex virus infections: Epidemiology, pathogenesis, symptomatology, diagnosis, and management. J Am Acad Dermatol, 57, 737-763.
  • Fernandez J, Alvarez JA, Lopez JA, 1997. Thiobarbituric acid test for monitoring lipid oxidation in meat. Food Chem, 59, 345-353.
  • Gullberg RC, Jordan Steel J, Moon SL, Soltani E, Geiss BJ, 2014. Oxidative stress influences positive strand RNA virus genome synthesis and capping. Virology, 12, 475, 219-229.
  • Hadigal S, Shukla D, 2013. Exploiting Herpes Simplex Virus entry for novel therapeutics. Viruses, 5, 1447-1465.
  • Hill JM, Lukiw WJ, Gebhardt BM, Higaki S, Loutsch JM, Myles ME, Thompson HW, Kwon BS, Bazan NG, Kaufman HE, 2001. Gene expression analyzed by microarrays in HSV-1 latent mouse trigeminal ganglion following heat stress. Virus Genes, 23, 273-280.
  • Jones C, 2013. Bovine Herpes Virus 1 (BHV-1) and Herpes Simplex Virus type 1 (HSV-1) romote survival of latently infected sensory neurons, in part by inhibiting apoptosis. J Cell Death, 6, 1-16.
  • Kleymann G, 2003. Novel agents and strategies to treat herpes simplex virus infections. Expert Opin Investig Drugs, 12, 65-183. Lykkesfeldt J, 2007. Malondialdehyde as biomarker of oxidative damage to lipids caused by smoking. Clin Chim Acta, 380, 50-58. Ma JZ, Russell TA, Spelman T, Carbone FR, Tscharke DC, 2014. Lytic gene expression is frequent in HSV-1 latent infection and correlates with the engagement of a cell-intrinsic transcriptional response. PLoS Pathog, 24, 10.
  • Mark HD, Nanda JP, Roberts J, Rompalo A, Melendez JH, Zenilman J, 2007. Performance of focus ELISA tests for HSV- 1 and HSV-2 antibodies among university students with no history of genital herpes. Sex Transm Dis, 34, 681- 685.
  • Murphy FA, Gibbs EPJ, Horzinek MC, Studdert MJ, 1999. Viral Taxonomy and Nomenclature, Veterinary Virology, 3th edition, Academic Press, USA, pp: 34-46.
  • Nagy TV, Olson SJ, Nagy KV, Montine TJ, Dermody TS, 2000. Herpes Simplex Virus Type 1 latency in the murine nervous system is associated with oxidative damage to neurons. Virology, 278, 309-321.
  • Nguyen AD, Itoh S, Jeney V, Yanagisawa H, Fujimoto M, UshioFukai M, Fukai T, 2004. Fibulin-5 is a novel binding protein for extracellular superoxide dismutase. Circ Res, 95, 1067- 1074.
  • Olagnier D, Peri S, Steel C, van Montfoort N, Chiang C, Beljanski V, Slifker M, He Z, Nichols CN, Lin R, Balachandran S, Hiscott J, 2014. Cellular oxidative stress response controls the antiviral and apoptotic programs in dengue virus-infected dendritic cells. PLoS Pathog, 18, 10(12), e1004566. doi: 10.1371/journal.ppat.1004566.
  • Paracha UZ, Fatima K, Alqahtani M, Chaudhary A, Abuzenadah A, Damanhouri G, Qadri I, 2013. Oxidative stress and hepatitis C virus. Virol J, 10, 251.
  • Paul D, Bartenschlager R, 2015. A sensor at the lipid - protein interface: Lipid peroxidation controls hepatitis C virus replication. Hepatology, 61,1083-1085.
  • Tabakoğlu E, Durgut R, 2013. Veteriner hekimlikte oksidatif stres ve bazı önemli hastalıklarda oksidatif stresin etkileri. AVKAE Derg, 3, 69-75.
  • Turrens JF, 1991. The potential of antioxidant enzymes as pharmacological agents in vivo. Xenobiotica, 21, 1033- 1040.
  • Yamane D, McGivern DR, Wauthier E, et al., 2014. Regulation of the hepatitis C virus RNA replicase by endogenous lipid peroxidation. Nat Med, 20, 927-935.
  • Yazar E, Traş B, 2001. Effects of fluoroquinolone antibiotics on hepatic superoxide dismutase and glutathione peroxidase activities in healthy and experimentally induced peritonitis mice. Revue Med Vet, 152, 235-238.
  • Yerer MB, Aydoğan S, 2000. Oksidatif stres ve antioksidanlar. EU J Health Sci, 9, 49-53.

Antioxidant enzyme activities in Vero cell line inoculated with Herpes Simplex Virus type 1

Year 2015, Volume: 31 Issue: 2, 122 - 126, 01.06.2015

Abstract

Aim: The aim of the present study was to determine of antioxidant capacity/oxidative stress biomarkers in Vero cell line inoculated with Herpes Simplex Virus type 1 (HSV-1).Materials and Methods: Cell supernatants were collected during 21 hours (15 min interval at first hour, 1 hour interval by 6th hour, 30 min interval at 6th -10th hours, 1 hour interval at 6th-21th hour) after HSV-1 inoculations. Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) enzymes, glutathione (GSH) and malondialdehyde (MDA) values were analyzed from the test media by commercially available ELISA kits. In addition to this, cytopathogenic effects (CPE) of HSV-1 in cell culture were evaluated periodically by invert microscope.Results: In this research, CPE of HSV-1 was observed at 6th hour post-inoculation. Maximum and minimum levels of SOD were determined at 17th and 1st hours, respectively. Minimum levels of CAT and GPX were determined at 18th and 7th hour, respectively. Minimum level of GSH was determined at first 30 minutes whereas maximum level of GSH was measured at 10th hours. MDA levels suddenly increased at 3rd hours and MDA reached maximum level at 18th hour. The SOD and GSH levels, important markers of oxidative stress, were reduced on HSV-1 before the formation CPE.Conclusion: It is concluded that lipid peroxidation may occur in the pathogenesis of HSV-1 and antioxidant treatment may be useful in the therapy of HSV-1 infection.

References

  • Ağaçfidan A, 2012. Cinsel yolla bulaşan hastalıklarda laboratuvarda tanı olanakları. ANKEM Derg, 26, 189-197.
  • Ajuyah AO, Fenton TW, Hardin RT, Sim JS, 1993. Measuring lipid oxidation volatiles in meats. J Food Sci, 58, 70-273. Amorati R, Valgimigli L, 2014. Advantages and limitations of common testing methods for antioxidants. Free Radic Res, 6, 1-38.
  • Avci O, Yavru S, Dik I. Determination of lipid peroxidation biomarkers in Vero cell line inoculated with Bovine Ephemeral Fever Virus. Eurasian J Vet Sci, 2014, 30, 4, 217-221.
  • Beck MA, Handy J, Levander OA, 2000. The role of oxidative stress in viral infections. Ann N Y Acad Sci, 917, 906-912.
  • Bölükbaş C, Bölükbaş FF, Horoz M, Aslan M, Çelik H, Erel O, 2005. Increased oxidative stress associated with the severity of the liver disease in various forms of hepatitis B virus infection. BMC Infect Dis, 31, 95.
  • Brady RC, Bernstein DI, 2004. Treatment of herpes simplex virus infections. Antiviral Res, 61, 73-81.
  • Chandrasena LG, Peiris H, Kamani J, Wanigasuriya P, Jayaratne SD, Wijayasiri WA, Wijesekara GU, 2014. Antioxidants in patients with dengue viral infection. Southeast Asian J Trop Med Public Health, 45, 1015-1022.
  • Cusini M, Ghislanzoni M, 2001. The importance of diagnosing genital herpes. J Antimicrob Chemother, 47, 9-16.
  • Egan KP, Wu S, Wigdahl B, Jennings SR, 2013. Immunological control of herpes simplex virus infections. J Neurovirol, 19, 328-345.
  • Fatahzadeh M, Schwartz RA, 2007. Human herpes simplex virus infections: Epidemiology, pathogenesis, symptomatology, diagnosis, and management. J Am Acad Dermatol, 57, 737-763.
  • Fernandez J, Alvarez JA, Lopez JA, 1997. Thiobarbituric acid test for monitoring lipid oxidation in meat. Food Chem, 59, 345-353.
  • Gullberg RC, Jordan Steel J, Moon SL, Soltani E, Geiss BJ, 2014. Oxidative stress influences positive strand RNA virus genome synthesis and capping. Virology, 12, 475, 219-229.
  • Hadigal S, Shukla D, 2013. Exploiting Herpes Simplex Virus entry for novel therapeutics. Viruses, 5, 1447-1465.
  • Hill JM, Lukiw WJ, Gebhardt BM, Higaki S, Loutsch JM, Myles ME, Thompson HW, Kwon BS, Bazan NG, Kaufman HE, 2001. Gene expression analyzed by microarrays in HSV-1 latent mouse trigeminal ganglion following heat stress. Virus Genes, 23, 273-280.
  • Jones C, 2013. Bovine Herpes Virus 1 (BHV-1) and Herpes Simplex Virus type 1 (HSV-1) romote survival of latently infected sensory neurons, in part by inhibiting apoptosis. J Cell Death, 6, 1-16.
  • Kleymann G, 2003. Novel agents and strategies to treat herpes simplex virus infections. Expert Opin Investig Drugs, 12, 65-183. Lykkesfeldt J, 2007. Malondialdehyde as biomarker of oxidative damage to lipids caused by smoking. Clin Chim Acta, 380, 50-58. Ma JZ, Russell TA, Spelman T, Carbone FR, Tscharke DC, 2014. Lytic gene expression is frequent in HSV-1 latent infection and correlates with the engagement of a cell-intrinsic transcriptional response. PLoS Pathog, 24, 10.
  • Mark HD, Nanda JP, Roberts J, Rompalo A, Melendez JH, Zenilman J, 2007. Performance of focus ELISA tests for HSV- 1 and HSV-2 antibodies among university students with no history of genital herpes. Sex Transm Dis, 34, 681- 685.
  • Murphy FA, Gibbs EPJ, Horzinek MC, Studdert MJ, 1999. Viral Taxonomy and Nomenclature, Veterinary Virology, 3th edition, Academic Press, USA, pp: 34-46.
  • Nagy TV, Olson SJ, Nagy KV, Montine TJ, Dermody TS, 2000. Herpes Simplex Virus Type 1 latency in the murine nervous system is associated with oxidative damage to neurons. Virology, 278, 309-321.
  • Nguyen AD, Itoh S, Jeney V, Yanagisawa H, Fujimoto M, UshioFukai M, Fukai T, 2004. Fibulin-5 is a novel binding protein for extracellular superoxide dismutase. Circ Res, 95, 1067- 1074.
  • Olagnier D, Peri S, Steel C, van Montfoort N, Chiang C, Beljanski V, Slifker M, He Z, Nichols CN, Lin R, Balachandran S, Hiscott J, 2014. Cellular oxidative stress response controls the antiviral and apoptotic programs in dengue virus-infected dendritic cells. PLoS Pathog, 18, 10(12), e1004566. doi: 10.1371/journal.ppat.1004566.
  • Paracha UZ, Fatima K, Alqahtani M, Chaudhary A, Abuzenadah A, Damanhouri G, Qadri I, 2013. Oxidative stress and hepatitis C virus. Virol J, 10, 251.
  • Paul D, Bartenschlager R, 2015. A sensor at the lipid - protein interface: Lipid peroxidation controls hepatitis C virus replication. Hepatology, 61,1083-1085.
  • Tabakoğlu E, Durgut R, 2013. Veteriner hekimlikte oksidatif stres ve bazı önemli hastalıklarda oksidatif stresin etkileri. AVKAE Derg, 3, 69-75.
  • Turrens JF, 1991. The potential of antioxidant enzymes as pharmacological agents in vivo. Xenobiotica, 21, 1033- 1040.
  • Yamane D, McGivern DR, Wauthier E, et al., 2014. Regulation of the hepatitis C virus RNA replicase by endogenous lipid peroxidation. Nat Med, 20, 927-935.
  • Yazar E, Traş B, 2001. Effects of fluoroquinolone antibiotics on hepatic superoxide dismutase and glutathione peroxidase activities in healthy and experimentally induced peritonitis mice. Revue Med Vet, 152, 235-238.
  • Yerer MB, Aydoğan S, 2000. Oksidatif stres ve antioksidanlar. EU J Health Sci, 9, 49-53.
There are 28 citations in total.

Details

Other ID JA59UV58VE
Journal Section Research
Authors

Sibel Yavru This is me

Oguzhan Avci This is me

İrmak Dik This is me

Kamil Atli This is me

Publication Date June 1, 2015
Published in Issue Year 2015 Volume: 31 Issue: 2

Cite

APA Yavru, S., Avci, O., Dik, İ., Atli, K. (2015). Antioxidant enzyme activities in Vero cell line inoculated with Herpes Simplex Virus type 1. Eurasian Journal of Veterinary Sciences, 31(2), 122-126.
AMA Yavru S, Avci O, Dik İ, Atli K. Antioxidant enzyme activities in Vero cell line inoculated with Herpes Simplex Virus type 1. Eurasian J Vet Sci. June 2015;31(2):122-126.
Chicago Yavru, Sibel, Oguzhan Avci, İrmak Dik, and Kamil Atli. “Antioxidant Enzyme Activities in Vero Cell Line Inoculated With Herpes Simplex Virus Type 1”. Eurasian Journal of Veterinary Sciences 31, no. 2 (June 2015): 122-26.
EndNote Yavru S, Avci O, Dik İ, Atli K (June 1, 2015) Antioxidant enzyme activities in Vero cell line inoculated with Herpes Simplex Virus type 1. Eurasian Journal of Veterinary Sciences 31 2 122–126.
IEEE S. Yavru, O. Avci, İ. Dik, and K. Atli, “Antioxidant enzyme activities in Vero cell line inoculated with Herpes Simplex Virus type 1”, Eurasian J Vet Sci, vol. 31, no. 2, pp. 122–126, 2015.
ISNAD Yavru, Sibel et al. “Antioxidant Enzyme Activities in Vero Cell Line Inoculated With Herpes Simplex Virus Type 1”. Eurasian Journal of Veterinary Sciences 31/2 (June 2015), 122-126.
JAMA Yavru S, Avci O, Dik İ, Atli K. Antioxidant enzyme activities in Vero cell line inoculated with Herpes Simplex Virus type 1. Eurasian J Vet Sci. 2015;31:122–126.
MLA Yavru, Sibel et al. “Antioxidant Enzyme Activities in Vero Cell Line Inoculated With Herpes Simplex Virus Type 1”. Eurasian Journal of Veterinary Sciences, vol. 31, no. 2, 2015, pp. 122-6.
Vancouver Yavru S, Avci O, Dik İ, Atli K. Antioxidant enzyme activities in Vero cell line inoculated with Herpes Simplex Virus type 1. Eurasian J Vet Sci. 2015;31(2):122-6.