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Kore'deki broyler tavuklarda Eimeria tenella ve Eimeriaa cervulina'nın internal transcribed spacer 1, ITS-1fragmentine dayalı filogenetik analizi ve karşılaştırılması

Year 2014, Volume: 30 Issue: 2, 89 - 94, 01.06.2014

Abstract

Amaç: Bu çalışmanın amacı Kore'de kanatlıları infekte eden Eimeria tenella, Eimeria acervulina'nın tespit edilmesini sağlayacak basit bir PZR gerçekleştirmek ve aynı ve komşu türler ile filogenetik ilişkisini belirlemektir. Gereç ve Yöntem: İki kanatlı Eimeria türünün (E. tenella ve E. acervulina) ribosomal RNA genlerinin internal transcribed spacer 1 (ITS-1) bölgelerinin sekans analizleri yapıldı ve sahadan elde edilen örneklerle bunlar arasında filogenetik ilişki analiz edildi.Bulgular: Kore’deki E. tenella ve E. acervulina'ların 273-bp ve 147-bp uzunluktaki sekansları kısmi nükleotid sekansları kullanılarak elde edilen filogramlar ile benzerlik gösterdi. Aynı türler içinde sekanslar E. tenella için %98 ve E. necatrix için %89 homologdur. Aynı türler içinde homoloji E. acervulina için %97, E. maxima için %77 ve E. mitis için %76 olarak belirlendi. Her bir Eimarei türünün ITS bölgesi yeterli türler-arası dizi varyasyonuna sahipti ve kullanılan primer Kore'deki herbir isolattan amplifikasyon yapmak için yeterli idi. Öneri: Bu çalışmada kullanılan moleküler metot benzer Eimeria türleri arasında ayrım yapmak için kullanışlıdır ve kanatlı coccidial enfeksiyonlarının epidemiyolojisi ve teşhisi için faydalı metodlar sunmaktadır

References

  • Allen PC, Fetterer RH, 2002. Recent advances in biology and immunobiology of Eimeria species and in diagnosis and control of infection with these coccidian parasites of poultry. Clin Microbiol Rev, 15, 58-65.
  • Allen PC, Jenkins MC, Miska KB, 2005. Cross protection studies with Eimeria maxima strains. Parasitol Res, 97, 179-185.
  • Bhaskaran MS, Venkatesan L, Aadimoolam R, Tirunelveli Jayagopal H, Sriraman R, 2010. Sequence diversity of internal transcribed spacer-1 (ITS-1) region of Eimeria infecting chicken and its relevance in species identification from Indian field samples. Parasitol Res, 106, 513-521.
  • Bornstein S, Gluecks IV, Younan M, Thebo P, Mattsson JG, 2008. Isospora orlovi infection in suckling dromedary camel calves (Camelus dromedarius) in Kenya. Vet Parasitol, 152, 194-201.
  • Daszak P,1999. Zoite migration during Eimeria tenella infection: Parasite adaptation to host defences. Parasitology Today, 15, 67-72.
  • Del Cacho E, Gallego M, Lopez-Bernad F, Quilez J, SanchezAcedo C, 2004. Expression of anti-apoptotic factors in cells parasitized by second-generation schizonts of Eimeria tenella and Eimeria necatrix. Vet Parasitol, 125, 287-300.
  • Doran DJ, Farr MM,1962. Excystation of the poultry coccidium, Eimeria acervulina. J Protozool, 9, 154-161.
  • Dunn A, Keymer A, 1986. Factors affecting the reliability of the McMaster technique. J Helminthol, 60, 260-262.
  • Fernandez S, Pagotto AH, Furtado MM, Katsuyama AM, Madeira AM, Gruber A, 2003. A multiplex PCR assay for the simultaneous detection and discrimination of the seven Eimeria species that infect domestic fowl. Parasitology, 127, 317-325.
  • Haug A, Thebo P, Mattsson JG, 2007. A simplified protocol for molecular identification of Eimeria species in field samples. Vet Parasitol, 146, 35-45.
  • Johnson J, Reid WM,1970. Anticoccidial drugs: lesion scoring techniques in battery and floor-pen experiments with chickens. Exp Parasitol, 28, 30-36.
  • Lee BH, Kim WH, Jeong J, Yoo J, Kwon YK, Jung BY, Kwon JH, Lillehoj HS, Min W, 2010. Prevalence and cross-immunity of Eimeria species on Korean chicken farms. J Vet Med Sci, 72, 985-989.
  • Park SS1, Lillehoj HS, Allen PC, Park DW, FitzCoy S, Bautista DA, Lillehoje EP, 2008. Immunopathology and cytokine
  • responses in broiler chickens coinfected with Eimeria maxima and Clostridium perfringens using an animal model of necrotic enteritis. Avian Dis, 52, 14-22.
  • Morehouse NF, Baron RR,1970. Coccidiosis: evaluation of coccidiostats by mortality, weight gains, and fecal scores. Exp Parasitol, 28, 25-29.
  • Morgan JA, Morris GM, Wlodek BM, Byrnes R, Jenner M, Constantinoiu CC, Anderson GR, Lew-Tabor AE, Molloy JB, Gasser RB, Jorgensen WK, 2009. Real-time polymerase chain reaction (PCR) assays for the specific detection and quantification of seven Eimeria species that cause coccidiosis in chickens. Mol Cell Probes, 23, 83-89.
  • Morris GM, Gasser RB, 2006. Biotechnological advances in the diagnosis of avian coccidiosis and the analysis of genetic variation in Eimeria. Biotechnol Adv, 24, 590-603.
  • Samarasinghe B, Johnson J, Ryan U, 2008. Phylogenetic analysis of Cystoisospora species at the rRNA ITS1 locus and development of a PCR-RFLP assay. Exp Parasitol,118, 592- 595.
  • Schnitzler BE, Thebo PL, Tomley FM, Uggla A, Shirley MW, 1999. PCR identification of chicken Eimeria: a simplified read-out. Avian Patholo, 28, 89-93.
  • Swinkels WJ, Post J, Cornelissen JB, Engel B, Boersma WJ, Rebel JM, 2007. Immune responses to an Eimeria acervulina infection in different broilers lines. Vet Immunol Immunopathol, 117, 26-34.
  • Tamura K, Dudley J, Nei M, Kumar S, 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol, 24, 1596-1599.
  • Tsuji N, Kawazu S, Ohta M, Kamio T, Isobe T, Shimura K, Fujisaki K,1997. Discrimination of eight chicken Eimeria species using the two-step polymerase chain reaction. J Parasitol, 83, 966-970.
  • Yun CH, Lillehoj HS, Choi KD, 2000. Eimeria tenella infection induces local gamma interferon production and intestinal lymphocyte subpopulation changes. Infect Immun, 68, 1282-1288.
  • Zhao XM, Duszynski DW, Loker ES, 2001. A simple method of DNA extraction for Eimeria species. J Microb Methods, 44, 131-137.

Comparison and analysis of phylogenetic relationship based on internal transcribed spacer 1, ITS-1 fragment of Eimeria tenella and Eimeria acervulina from broiler chicken in Korea

Year 2014, Volume: 30 Issue: 2, 89 - 94, 01.06.2014

Abstract

Aim: The objective of the present study is the simple PCR assay that permits the detection of the Eimeria tenella, Eimeria acervulina that infect domestic fowl in Korea and making phylogenetic relation with in the same and neighboring species. Materials and Methods: The internal transcribed spacer 1 (ITS-1) region of ribosomal RNA genes of two poultry Eimeria species; E. tenella and E. acervulina were sequenced and analyzed the phylogenetic relationship among them from field isolates.Results: About the 273-bp and 147-bp sequence of ITS-1 of E. tenella and E. acervulina in Korea were similar reflected in the phylogram constructed using the partial nucleotide sequences. In case of E. tenella for homology searching the sequences among the same species was 98% and with E. necatrix was 89%. In case of E. acervulina sequence similarity within the same species was 97%, whereas the similarity E. maxima and E. mitis were 77% and 76%, respectively. ITS1 region of each Eimeria species had sufficient inter-specific sequence variation and the primer is sufficient to amplify each target Korean isolates. Conclusions: The molecular method examine in the present study appears useful for discriminating among similar Eimeria species providing useful methods for diagnosis and epidemiology of avian coccidial infection.

References

  • Allen PC, Fetterer RH, 2002. Recent advances in biology and immunobiology of Eimeria species and in diagnosis and control of infection with these coccidian parasites of poultry. Clin Microbiol Rev, 15, 58-65.
  • Allen PC, Jenkins MC, Miska KB, 2005. Cross protection studies with Eimeria maxima strains. Parasitol Res, 97, 179-185.
  • Bhaskaran MS, Venkatesan L, Aadimoolam R, Tirunelveli Jayagopal H, Sriraman R, 2010. Sequence diversity of internal transcribed spacer-1 (ITS-1) region of Eimeria infecting chicken and its relevance in species identification from Indian field samples. Parasitol Res, 106, 513-521.
  • Bornstein S, Gluecks IV, Younan M, Thebo P, Mattsson JG, 2008. Isospora orlovi infection in suckling dromedary camel calves (Camelus dromedarius) in Kenya. Vet Parasitol, 152, 194-201.
  • Daszak P,1999. Zoite migration during Eimeria tenella infection: Parasite adaptation to host defences. Parasitology Today, 15, 67-72.
  • Del Cacho E, Gallego M, Lopez-Bernad F, Quilez J, SanchezAcedo C, 2004. Expression of anti-apoptotic factors in cells parasitized by second-generation schizonts of Eimeria tenella and Eimeria necatrix. Vet Parasitol, 125, 287-300.
  • Doran DJ, Farr MM,1962. Excystation of the poultry coccidium, Eimeria acervulina. J Protozool, 9, 154-161.
  • Dunn A, Keymer A, 1986. Factors affecting the reliability of the McMaster technique. J Helminthol, 60, 260-262.
  • Fernandez S, Pagotto AH, Furtado MM, Katsuyama AM, Madeira AM, Gruber A, 2003. A multiplex PCR assay for the simultaneous detection and discrimination of the seven Eimeria species that infect domestic fowl. Parasitology, 127, 317-325.
  • Haug A, Thebo P, Mattsson JG, 2007. A simplified protocol for molecular identification of Eimeria species in field samples. Vet Parasitol, 146, 35-45.
  • Johnson J, Reid WM,1970. Anticoccidial drugs: lesion scoring techniques in battery and floor-pen experiments with chickens. Exp Parasitol, 28, 30-36.
  • Lee BH, Kim WH, Jeong J, Yoo J, Kwon YK, Jung BY, Kwon JH, Lillehoj HS, Min W, 2010. Prevalence and cross-immunity of Eimeria species on Korean chicken farms. J Vet Med Sci, 72, 985-989.
  • Park SS1, Lillehoj HS, Allen PC, Park DW, FitzCoy S, Bautista DA, Lillehoje EP, 2008. Immunopathology and cytokine
  • responses in broiler chickens coinfected with Eimeria maxima and Clostridium perfringens using an animal model of necrotic enteritis. Avian Dis, 52, 14-22.
  • Morehouse NF, Baron RR,1970. Coccidiosis: evaluation of coccidiostats by mortality, weight gains, and fecal scores. Exp Parasitol, 28, 25-29.
  • Morgan JA, Morris GM, Wlodek BM, Byrnes R, Jenner M, Constantinoiu CC, Anderson GR, Lew-Tabor AE, Molloy JB, Gasser RB, Jorgensen WK, 2009. Real-time polymerase chain reaction (PCR) assays for the specific detection and quantification of seven Eimeria species that cause coccidiosis in chickens. Mol Cell Probes, 23, 83-89.
  • Morris GM, Gasser RB, 2006. Biotechnological advances in the diagnosis of avian coccidiosis and the analysis of genetic variation in Eimeria. Biotechnol Adv, 24, 590-603.
  • Samarasinghe B, Johnson J, Ryan U, 2008. Phylogenetic analysis of Cystoisospora species at the rRNA ITS1 locus and development of a PCR-RFLP assay. Exp Parasitol,118, 592- 595.
  • Schnitzler BE, Thebo PL, Tomley FM, Uggla A, Shirley MW, 1999. PCR identification of chicken Eimeria: a simplified read-out. Avian Patholo, 28, 89-93.
  • Swinkels WJ, Post J, Cornelissen JB, Engel B, Boersma WJ, Rebel JM, 2007. Immune responses to an Eimeria acervulina infection in different broilers lines. Vet Immunol Immunopathol, 117, 26-34.
  • Tamura K, Dudley J, Nei M, Kumar S, 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol, 24, 1596-1599.
  • Tsuji N, Kawazu S, Ohta M, Kamio T, Isobe T, Shimura K, Fujisaki K,1997. Discrimination of eight chicken Eimeria species using the two-step polymerase chain reaction. J Parasitol, 83, 966-970.
  • Yun CH, Lillehoj HS, Choi KD, 2000. Eimeria tenella infection induces local gamma interferon production and intestinal lymphocyte subpopulation changes. Infect Immun, 68, 1282-1288.
  • Zhao XM, Duszynski DW, Loker ES, 2001. A simple method of DNA extraction for Eimeria species. J Microb Methods, 44, 131-137.
There are 24 citations in total.

Details

Other ID JA62GS36DG
Journal Section Research
Authors

Myung-Jo You This is me

Publication Date June 1, 2014
Published in Issue Year 2014 Volume: 30 Issue: 2

Cite

APA You, M.-J. (2014). Comparison and analysis of phylogenetic relationship based on internal transcribed spacer 1, ITS-1 fragment of Eimeria tenella and Eimeria acervulina from broiler chicken in Korea. Eurasian Journal of Veterinary Sciences, 30(2), 89-94.
AMA You MJ. Comparison and analysis of phylogenetic relationship based on internal transcribed spacer 1, ITS-1 fragment of Eimeria tenella and Eimeria acervulina from broiler chicken in Korea. Eurasian J Vet Sci. June 2014;30(2):89-94.
Chicago You, Myung-Jo. “Comparison and Analysis of Phylogenetic Relationship Based on Internal Transcribed Spacer 1, ITS-1 Fragment of Eimeria Tenella and Eimeria Acervulina from Broiler Chicken in Korea”. Eurasian Journal of Veterinary Sciences 30, no. 2 (June 2014): 89-94.
EndNote You M-J (June 1, 2014) Comparison and analysis of phylogenetic relationship based on internal transcribed spacer 1, ITS-1 fragment of Eimeria tenella and Eimeria acervulina from broiler chicken in Korea. Eurasian Journal of Veterinary Sciences 30 2 89–94.
IEEE M.-J. You, “Comparison and analysis of phylogenetic relationship based on internal transcribed spacer 1, ITS-1 fragment of Eimeria tenella and Eimeria acervulina from broiler chicken in Korea”, Eurasian J Vet Sci, vol. 30, no. 2, pp. 89–94, 2014.
ISNAD You, Myung-Jo. “Comparison and Analysis of Phylogenetic Relationship Based on Internal Transcribed Spacer 1, ITS-1 Fragment of Eimeria Tenella and Eimeria Acervulina from Broiler Chicken in Korea”. Eurasian Journal of Veterinary Sciences 30/2 (June 2014), 89-94.
JAMA You M-J. Comparison and analysis of phylogenetic relationship based on internal transcribed spacer 1, ITS-1 fragment of Eimeria tenella and Eimeria acervulina from broiler chicken in Korea. Eurasian J Vet Sci. 2014;30:89–94.
MLA You, Myung-Jo. “Comparison and Analysis of Phylogenetic Relationship Based on Internal Transcribed Spacer 1, ITS-1 Fragment of Eimeria Tenella and Eimeria Acervulina from Broiler Chicken in Korea”. Eurasian Journal of Veterinary Sciences, vol. 30, no. 2, 2014, pp. 89-94.
Vancouver You M-J. Comparison and analysis of phylogenetic relationship based on internal transcribed spacer 1, ITS-1 fragment of Eimeria tenella and Eimeria acervulina from broiler chicken in Korea. Eurasian J Vet Sci. 2014;30(2):89-94.