First molecular evidence of Coxiella spp. from Rhipicephalus sanguineus ticks in Cebu, Philippines

Year 2014, Volume: 30 Issue: 1, 35 - 38, 01.03.2014

Adrian P. Ybañez

Abstract

Coxiella species are known to be potentially pathogenic tickborne
organisms. This study was aimed to investigate the
presence of Coxiella sp. in Rhipicephalus sanguineus ticks in
Cebu, Philippines by molecular based techniques. A total of
164 ticks were collected from 36 dogs and analyzed by a Nested
Polymerase Chain Reaction (PCR) for the amplification of
partial 16S ribosomal RNA gene region. Nine ticks (5.5%) obtained
from 5 dogs were found positive. Sequencing revealed
partial DNA fragments of Coxiella spp. which were 96.3% and
98.1-100% identical to Coxiella burnetii and Coxiella sp. endosymbiont,
respectively. The detected DNA fragments also
shared 98.3-100% identities with each other. This study is
the first report on the existence of Coxiella spp. from Rhipicephalus
sanguineus ticks in Cebu, Philippines.

Keywords

Coxiella sp., Rhipicephalus sanguineus, Philippines

Filipinler, Cebu’dan elde edilen Rhipicephalus sanguineus kenelerinden Coxiella spp. türlerinin moleküler olarak ilk belirlenmesi

Abstract

Coxiella türleri kene ısırması ile geçebilen patojenik etkendir.
Mevcut araştırma Filipinler, Cebu’da Rhipicephalus sanguineus
kenelerinden Coxielle türlerinin varlığını moleküler tekniklerle
belirlemek için yapıldı. Otuzaltı köpekten elde edilen
toplam 164 adet kene Nested Polimeraz Zincir Reaksiyonu
(PCR) ile kısmi 16S ribozomal RNA gen bölgesi çoğaltılarak
analiz edildi. Beş adet köpekten elde edilen 9 adet kene
pozitif (%5.5) bulundu. Sekans analizi sonucunda Coxiella
burnetii (%96.3) ve Coxiella sp. endosymbiont (%98.1-100)
ile benzer Coxiella spp.'ya ait DNA fragmentleri tespit edildi.
Belirlenen DNA fragmentleri birbirleri ile de %98.3-100
oranında benzerdi. Bu çalışma Filipinler Cebu'da Rhipicephalus
sanguineus kenelerinden elde edilen Coxielle spp. varlığı-
nı gösteren ilk araştırmadır.

Keywords

Coxiella sp., Rhipicephalus sanguineus, Filipinler

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