Identification and antibiotic susceptibility of coagulase negative staphylococci isolated from dairy cows with subclinical mastitis

Year 2014, Volume: 30 Issue: 1, 14 - 19, 01.03.2014

H. Hüseyin Hadimli Zafer Sayın Osman Erganiş Kürşat Kav Aslı Sakmanoğlu

Abstract

Aim: This study was aimed to identify of coagulase negative staphylococci (CNS) isolated from bovine milk samples with subclinical mastitis and to determine susceptibility of microorganisms to antibiotics. Materials and Methods: Totally, 286 CNS isolates from milk samples were identified according to colony morphology, haemolysis and biochemical properties. Results: Out of isolates, 40 (14.38%) were identified as S. simulans, 31 (11.15%) S. epidermidis, 54 (19.42%) S. chromogenes, 31 (11.15%) S. xylosus, 22 (7.91%) S. caprae, 19 (6.83%) S. warneri, 18 (6.47%) S. haemolyticus, 13 (4.67%) S. cheiferi subsp. coagulans, 10 (3.59%) S. cohneii subsp. urealyticus, 10 (3.59%) S. saprophyticus, 6 (2.15%) S. gallinarum, 7 (2.51%) S. hominis, 4 (1.43%) S. hyicus, 2 (0.71%) S. cheiferi subsp. cheiferi, 9 (3.23%) S. lentus and 2 (0.71%) S. sciuri. The susceptibilities of CNS were determined to be variable to different antimicrobial agents. Conclusions: This study was shown that identification of CNS isolated from dairy cows with subclinical mastitis should be strictly performed.

Keywords

Coagulase negative staphylococci, mastitis, dairy cow

Subklinik mastitisli süt ineklerinden izole edilen koagulaz negatif stafilokokların identifikasyonu ve antibiyotiklere duyarlılıkları

Abstract

Amaç: Bu çalışmada subklinik mastitisli süt ineklerinden izole edilen koagulaz negatif stafilokokların (KNS) identifikasyonu ve antibiyotiklere duyarlılıklarının belirlenmesi amaçlandı. Gereç ve Yöntem: Subklinik mastitisli inek sütlerinden izole edilen toplam 286 KNS izolatı koloni morfolojisi, hemoliz ve biyokimyasal özelliklerine göre identifiye edildi.Bulgular: İzolatların 40’ı S. simulans (%14.98), 31’i S. epidermidis (%11.15), 54’ü S. chromogenes (%19,42), 31’i S. xylosus (%11.15), 22’si S. caprae (%7.91), 19’u S. warneri (%6.83), 18’i S. haemolyticus (%6.47), 13’ü S. cheiferi subsp. coagulans (%4.67), 10’u S. cohneii subsp. urealyticus (%3.59), 10’u S. saprophyticus (%3.59), 6’sı S. gallinarum (%2.15), 7’si S. hominis (%2.51), 4’ü S. hyicus (%1.43), 2’si S. cheiferi subsp. cheiferi (%0.71), 9’u S. lentus (%3.23) ve 2’si S. sciuri (%0.71) olarak identifiye edildi. KNS’ların farklı antibiyotiklere duyarlılıklarının değişken olduğu gözlendi. Öneri: Bu çalışma, subklinik mastitisli inek sütlerinden izole edilen KNS’ların tam identifikasyonlarının yapılması gerektiğini gösterdi.

Keywords

Koagulaz negatif stafilokok, mastitis, süt ineği

References

• Aerestrup FM, Larsen HD, Jensen NE, 1999. Characterization of Staphylococcus simulans strains isolated from cases of bovine mastitis. Vet Microbiol, 66, 165-170.
• Alaçam E, Tekeli T, Erganiş O, İzgi C, 1989. İnek ve mandalarda subklinik mastitislerin tanısı, etkenlerin izolasyonu ve bunlara karşı etkili antibiyotiklerin belirlenmesi. Eurasian J Vet Sci, 5, 81-91.
• Archer GL, Climo MW, 1994. Antimicrobial susceptibility of coagulase-negative staphylococci. Antimicrob Agents Chemother, 38, 2231-2237.
• Arslan E, Uçan US, 2005. Mastitisli ineklerden izole edilen Staphylococcus aureus ve S. intermedius suşlarının slime üretiminin araştırılması. Eurasian J Vet Sci, 21, 1-2, 61-63.
• Ataee O, Hovareshti P, Bolourchi M, Niasari-Naslaji A, Barin A, 2007. Prevalence and antimicrobial susceptibilities of coagulase negative staphylococci isolated from mammary secretions in pregnant Holstein heifers. Iranian J Vet Res, 8, 3, 270-274.
• Ateş M, Erganiş O, Çorlu M, Serpek B, 1991. Konya yöresindeki mastitisli ineklerden elde edilen süt örneklerinin mikrobiyel florası ve LDH aktivitesi. Doğa Tr Vet Animal Sci, 47, 152-157.
• Bauer AW, Kirby WMM, Sherris JC, Turck M, 1966. Antibiotic susceptibilty testing by a standardized single disk method. Am J Clin Path, 45, 493.
• Brinda M, Herman V, Fodor J, 2010. Phenotypic characterization of coagulase negative staphylocci isolated from mastitis milk in cows. Luc Stiin Med Vet, 18, 1, 97-101.
• Christensen GD, Simpson WA, Bisno AL, Beachey EH, 1982. Adherence of slime producing strains of Staphylococcus epidermidis to smooth surfaces. Infect Immun, 37, 318- 326.
• Dufour S, Dohoo IR, Barkema HW, DesCoteaux L, Devries TJ, Reyher KK, Roy JP, Scholl DT, 2012. Epidemiology of coagulase-negative staphylococci intramammary infection in dairy cattle and the effect of bacteriological culture misclassification. J Dairy Sci, 95, 6, 3110-3124.
• Davenport DS, Masanari KM, Pfaller MA, Bae MJ, Streed SA, Hierholzer WS, 1986. Usefulness of a test for slime production as a marker for clinically significant infections with coagulase negative staphylococci. J Infect Dis, 153, 2, 332- 339.
• Erganiş O, Kuyucuoğlu Y, Ok Ü, 1995. İnek ve koyun mastitislerine sebep olan koagulaz negatif ve pozitif stafilokokların biyotiplendirilmesi. Veterinarium, 6, 23-27.
• Eskiizmirliler S, Öncel T, 2001. İzmir bölgesinde görü- len mastitis olgularından izole edilen β-laktamaz (+) ve β-laktamaz (-) Staphylococcus türlerinin amoksisilin ve amoksisilin+klavulonik asit’e duyarlılıklarının karşılaştı- rılması. Süt İnekçiliğinde Mastitis Sempozyumu, 4-5 Ma-yıs, Burdur.
• Gentilini E, Denamiel G, Betancor A, Rebuelto M, Rodriquez Fermepin M, De Torrest RA, 2002. Antimicrobial susceptibility of coagulase-negative staphylococci isolated from bovine mastitis in Argentina. J Dairy Sci, 85, 1913-1917.
• Hadimli HH, Ateş M, Güler L, Kav K, Öncel T, 2001. Mastitisli süt ineklerinden izole edilen stafilokokların β-Laktamaz aktiviteleri ve antibiyotiklere duyarlılıkları. Eurasian J Vet Sci, 4, 21-25.
• Havelka B, 1990. Coagulase negative staphylocci isolated from milk of dairy cows. Vet Med, 35, 12, 713-716.
• Kaliwal BB, Sadashiv SD, Kurjogi MM, Sanakal RD, 2011. Prevalence and antimicrobial susceptibility of coagulasenegative staphylococci isolated from bovine mastitis. Vet World, 4, 4, 158-161.
• Kaynarca S, Türkyılmaz S, 2010. Sığır mastitislerinden izole edilen stafilokoklarda metisilin direnci ve slaym pozitifliği. Kafkas Uni, Vet Fak Derg, 16, 4, 567-572.
• Keskin O, Altay G, Akan M, 2003. Farklı hayvansal kaynaklardan izole edilen koagulaz negatif stafilokoklarda slime üretimi ve aderans. Turk J Vet Anim Sci, 27, 252-257.
• Kırkan Ş, Göksoy EÖ, Kaya O, 2005. Identification of antimicrobial susceptibility of Staphylococcus aureus and coagulase negative staphylocci from bovine mastitis in the Aydın region of Turkey. Turk J Vet Anim Sci, 29, 791-796.
• Kudinha T, Simango C, 2002. Prevalence of coagulase negative staphylococci in bovine mastitis in Zimbabwe. Tysdskr S Afr Vet Res, 73, 2, 62-65.
• Luthje P, Schwarz S, 2006. Antimicrobial resistance of coagulase-negative staphylococci from bovine subclinical mastitis with particular reference to macrolide–lincosamide resistance phenotypes and genotypes. J Antimicrob Chemother, 57, 966-969.
• Matthews KR, Harmon RJ, Langlois BE, 1991. Effect of naturally occurring coagulase-negative staphylococci infections on new infections by mastitis pathogens in the bovine. J Dairy Sci, 74, 1855-1859.
• Moon JS, Lee AR, Kang HM, Lee ES, Kim MN, Paik YH, Park YH, Joo YS, Koo HC, 2007. Phenotypic and genetic antibiogram of methicillin-resistant staphylococci isolated from bovine mastitis in Korea. J Dairy S, 90, 1176-1185.
• NCCLS, 2003. National committe for clinical laboratory standarts: Performance standarts for antimicrobial susceptibility testing. Eighth Edition, NCCLS document M2-A8 volume 23, no 1.
• Oliver SP, Jayarao BM, 1997. Coagulase-negative staphylococcal intramammary infections in cows and heifers during the nonlactating and periparturient periods. J Vet Med B, 44, 355-363.
• Pengov A, 2001. The role of coagulase negative Staphylococcus spp. and associated somatic cell counts in the ovine mammary gland. J Dairy Res, 84, 572-574.
• Piessens V, Van Coillie E, Verbist B, Supre K, Braem G, Van Nuffel A, 2011. Distribution of coagulase-negative Staphylococcus species from milk and environment of dairy cows differs between herds. J Dairy Sci, 94, 6, 2933-2944.
• Pyölara S, Taponen S. 2009. Coagulase-negative staphylococciEmerging mastitis pathogens. Vet Microbiol, 134, 1-2, 3-8.
• Sampimon OC, Vernoji JC, Mevius DJ, Sol J, 2007. Sentivity of various antibiotics of coagulase negative staphylocci isolated from samples of milk from Dutch dairy cattle. Tijd V Dierg, 132, 6, 2000-2004.
• Schukken YH, Gonzalez RN, Tikofsky LL, Schulte HF, Santisteban CG, Welcome FL, Bennett GJ, Zurakowski MJ, Zadoks RN, 2009. CNS mastitis: Nothing to worry about? Vet Microbiol, 134, 9-14.
• Simojoki H, 2011. Bovine mastitis caused by coagulase negative staphylocci: Host response and bacterial factors. Doctora Thesis, University of Helsinki.
• Souli M, Giamarelllou H, 1998. Effects of slime produced by clinical ısolates of coagulase-negative staphylococci on activities of various antimicrobial agents, Antimicrob Agents Chemother, 42, 939-941.
• Taponen S, Simojoki H, Haveri M, Larsen HD, Pyorala S, 2006. Clinical characteristics and persistence of bovine mastitis caused by different species of coagulase-negative staphylococci identified with API or AFLP, Vet Microbiol, 115, 199-207.
• Taponen S, Koort J, Bjorkroth J, Saloniemi H, Pyölara S, 2007. Bovine mastitis caused by coagulase negative staphylocci may persist. ISAH, Tartu, Estonia, pp:476-479.
• Tenhagen BA, Koster G, Wallmann J, Heuwieser W, 2006. Prevalence of mastitis pathogens and their resistance against antimicrobial agents in dairy cows in Brandenburg, Germany. J Dairy Sci, 89; 2542–2551.
• Thorberg BM, 2008. Coagulase negative staphylococci in bovine subclinical mastitis. Licentiate Thesis, Swedish University, Uppsala.
• Thorberg BM, Danielsson-Tham ML, Emanuelson UE, Persson Waller K, 2009. Bovine subclinical mastitis caused by different types of coagulase-negative staphylococci. J Dairy Sci, 92, 10, 4962-4970.
• Turutoglu H, Ercelik S, Ozturk D, 2006. Antibiotic resistance of Staphylococcus aureus and coagulase negative staphylococci isolated from bovine mastitis. Bull Vet Inst Pulawy, 50, 41-45.
• Winter P, Colditz IG, 2002. Immunological responses of the lactating ovine udder following experimental challenge with Staphylococcus epidermidis. Vet Immun Immunpathol, 89, 57-65.
• Zadoks RN, Watts JL, 2009. Species identification of coagulase-negative staphylococci: Genotyping is superior to phenotyping. Vet Microbiol, 134, 1-2, 20-2