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Türk sucuğunda Enterobacteriaceae ve Staphylococcaceae identifikasyonu

Year 2013, Volume: 29 Issue: 4, 192 - 197, 01.12.2013

Abstract

Amaç: Bu çalışmada, Afyonkarahisar’da beş farklı işletmede üretilen Türk sucuğu örneklerinde Enterobacteriaceae ve Staphylococcaceae familyalarına ait türlerin identifikasyonu yapıldı.Gereç ve Yöntem: Materyal olarak Afyonkarahisar'ın beş farklı bölgesinde üretilen Türk fermente sucukları kullanıldı. Enterobacteriaceae sayımı için Violet Red Bile Glucose Agar ve (ISO 21528-2 2004), Staphylococcaceae sayımı için Baird Parker Agar (FDA BAM 2001) kullanıldı. Enterobacteriaceae tür düzeyinde API-20E test kiti (bioMérieux Inc API- 20 E 2006) ile Staphylococcaceae tür düzeyinde API-Staph test kiti (bioMérieux Inc API-Staph 2009) ile identifikasyonu yapıldı.Bulgular:Enterobacteriaceae türlerinin izolasyon ve idetifikasyonu yapıldı. Araştırmada 90 Sta phylococcus izolatı Türk sucuklarından izole edildi. İzole edilen Staphylococcaceae izolatları S. aureus (%12.94), S. epidermidis (%15.29), S. simulans (%12.94) ve S. carnosus (%11.76) olarak identifiye edildi. İzole edilen Enterobacteriaceae izolatları ise Citrobacter diversus (%5.96), Enterobacter sazazaki (%11.91), Enterobacter gergoviae (%17.87), Klepsiella ozaerae (%6.38), Salmonella arizonae (%6.38), Salmonella spp (%8.94), Escherichia coli (%8.94), Serratia marcescens (%6.81), Serratia liquefaciens (%6.38) ve Yersinia enterocolitica (%4.26) olarak identifiye edildi. Öneri: Türk sucuklarında Staphylococcaceae familyasına ait starter kültür olarak kullanılabilecek S. simulans ilk defa tespit edilmiştir. Ayrıca Türk sucuklarında tespit edilen Staphylococcaceae ve Enterobacteriaceae familyasına ait patojen bakteri türlerinin gıda güvenliği ve halk sağlığı açısından risk taşıdığını göstermektedir.

Identification of Enterobacteriaceae and Staphylococcaceae at Turkish sucuk

Year 2013, Volume: 29 Issue: 4, 192 - 197, 01.12.2013

Abstract

Aim: In this study, the species from Enterobacteriaceae and Staphylococcaceae families have been identified in Turkish fermented sausages samples of five different corporations which are produced in Afyonkarahisar.Materials and Methods: Turkish fermented sausages of five different corporations which are produced in Afyonkarahisar were used as the material. In order for the calculation of Enterobacteriaceae, inoculation was made into Violet Red Bile Glucose Agar medium (ISO 21528-2 2004) and for the calculation of Staphylococcaceae Baird Parker Agar medium (FDA BAM, 2001) was used. Enterobacteriaceae were identified at species level with API- 20 E test kit (bioMérieux Inc API-20 E 2006). Staphylococcaceae were identified with APIStaph test kit (bioMérieux Inc API-Staph 2009).Results: Isolation and identification of Staphylococcaceae and Enterobacteriaceae species in Turkish sausage flora were done. 90 Staphylococcus isolates were derived from the Turkish sausage samples in the research. S. aureus (12.94%), S. epidermidis (15.29%), S. simulans (12.94%) and S. carnosus (11.76%) from Staphylococcaceae family have been identified. 235 Enterobacteriaceae isolates from Turkish sausage samples were identified at the species level as Citrobacter diversus (5.96%), Enterobacter sazazaki (11.91%), Enterobacter gergoviae (17.87%), Klepsiella ozaerae (6.38%), Salmonella arizonae (6.38%), Salmonella spp. (8.94%), Escherichia coli (8.94%), Serratia marcescens (6.81%), Serratia liquefaciens (6.38%) and Yersinia enterocolitica (4.26%). Conclusion: S. simulans from Staphylococcaceae family which can be used as a starter culture has been found in Turkish sausages for the first time. Pathogenic bacterium species from Staphylococcaceae and Enterobacteriaceae families determined in Turkish sausages indicate a risk for food safety and public health.

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Details

Other ID JA64VF34UD
Journal Section Research
Authors

Mukadderat Gökmen This is me

Recep Kara This is me

Levent Akkaya This is me

Veli Gök This is me

Publication Date December 1, 2013
Published in Issue Year 2013 Volume: 29 Issue: 4

Cite

APA Gökmen, M., Kara, R., Akkaya, L., Gök, V. (2013). Identification of Enterobacteriaceae and Staphylococcaceae at Turkish sucuk. Eurasian Journal of Veterinary Sciences, 29(4), 192-197.
AMA Gökmen M, Kara R, Akkaya L, Gök V. Identification of Enterobacteriaceae and Staphylococcaceae at Turkish sucuk. Eurasian J Vet Sci. December 2013;29(4):192-197.
Chicago Gökmen, Mukadderat, Recep Kara, Levent Akkaya, and Veli Gök. “Identification of Enterobacteriaceae and Staphylococcaceae at Turkish Sucuk”. Eurasian Journal of Veterinary Sciences 29, no. 4 (December 2013): 192-97.
EndNote Gökmen M, Kara R, Akkaya L, Gök V (December 1, 2013) Identification of Enterobacteriaceae and Staphylococcaceae at Turkish sucuk. Eurasian Journal of Veterinary Sciences 29 4 192–197.
IEEE M. Gökmen, R. Kara, L. Akkaya, and V. Gök, “Identification of Enterobacteriaceae and Staphylococcaceae at Turkish sucuk”, Eurasian J Vet Sci, vol. 29, no. 4, pp. 192–197, 2013.
ISNAD Gökmen, Mukadderat et al. “Identification of Enterobacteriaceae and Staphylococcaceae at Turkish Sucuk”. Eurasian Journal of Veterinary Sciences 29/4 (December 2013), 192-197.
JAMA Gökmen M, Kara R, Akkaya L, Gök V. Identification of Enterobacteriaceae and Staphylococcaceae at Turkish sucuk. Eurasian J Vet Sci. 2013;29:192–197.
MLA Gökmen, Mukadderat et al. “Identification of Enterobacteriaceae and Staphylococcaceae at Turkish Sucuk”. Eurasian Journal of Veterinary Sciences, vol. 29, no. 4, 2013, pp. 192-7.
Vancouver Gökmen M, Kara R, Akkaya L, Gök V. Identification of Enterobacteriaceae and Staphylococcaceae at Turkish sucuk. Eurasian J Vet Sci. 2013;29(4):192-7.