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Nerium oleander distilatının antimikrobiyal etkisinin araştırılması

Year 2013, Volume: 29 Issue: 3, 150 - 152, 01.09.2013

Abstract

Amaç: Mevcut araştırmanın amacı Nerium oleander distilatının antimikrobial etkisini araştırmaktır. Gereç ve Yöntem: Toplanan Nerium oleander bitkisi tanımlandı ve onaylandı. Nerium oleander yıkandı ve doğrandı, sonrasında yeterli oranda su ilave edildi. Karışım distilastorde distile edildi. Bitki-distile su karışımı buharlaştırılmaya başlandıktan sonra buhar kısmı temiz cam kaplara ayrıştırıldı. Elde edilen Nerium oleander distilatı küçük cam şişeler içinde liyofilize edildi ve kuru materyal toplandı. Liyofilize kuru materyal 5 mg/mL olacak şekilde distile suda çözdürüldü ve Escherichia coli, Salmonella Typhimurium, Salmonella Enteritidis, Staphylococcus aureus, Metisilin Rezistans Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumonia, Listeria monocytogenes, Pseudomonas aeruginosa ve Candida ssp.’ya karşı antimikrobiyal etkisi çukur agardifüzyon metodu ile araştırıldı. Bulgular: Nerium oleander distilatının incelenen hiçbir mikroorganizmaya karşı etkili olmadığı belirlendi. Öneri: Nerium oleander distilatının kimyasal ekstraklarının aksine antimikrobiyal etkisinin olmadığı ifade edilebilir.

References

  • Balabanlı C, Albayrak S, Türk M, Yüksel O, 2006. Türkiye çayır meralarında bulunan bazı zararlı bitkiler ve hayvanlar üzerindeki etkileri. SDÜ Orman Fakültesi Dergisi, 2, 89-96.
  • Bas AL, Demirci S, Yazihan N, Uney K, Kaya EE, 2012. Nerium oleander distillate improves fat and glucose metabolism in high-fat diet-fed streptozotocin-induced diabetic rats. Int J Endocrinol, 2012, 1-10.
  • Baytop T, 1984. Türkiye’de Bitkilerle Tedavi, İ. Ü. Yayınları, No: 3255, İstanbul, Türkiye, p:411.
  • Bhuvaneshwari L, Arthy E, Anitha C, Dhanabalan K, Meena M, 2007. Phytochemical analysis & antibacterial activity of Nerium oleander. Anc Sci Life, 26, 24-28.
  • Dik B, Uney K, Ozdemir O, Demirci S, Yazıhan NA, Bas AL, 2012. Acute oral toxicity of Nerium oleander distilate in rats (Abstract). J Vet Pharmacol Therap, 35, suppl 3, 78- 102.
  • Gupta V, Mittal P, 2010. Phytochemical and pharmacological potential of Nerium oleander: A review. IJPS, 1, 3.
  • Hadizadeh I, Peivastegan B, Kolahi M, 2009. Antifungal activity of nettle (Urtica diocia L.), colocynth (Citrullus colocynthis L. Schrad), oleander (Nerium oleande L.) and konar (Ziziphus spina-christi L.) extracts on plants pathogenic fungi. Pak J Biol Sci, 12, 58-63.
  • Hussain I, Khattak MUR, Ullah R, Muhammad Z, Khan N, Khan FA, Ullah Z, Haider S, 2011. Phytochemicals screening and antimicrobial activities of selected medicinal plants of Khyberpakhtunkhwa Pakistan. Afr J Pharm Pharmacol, 5, 746-750.
  • Hussain MA, Gorsi MS, 2004. Antimicrobial activity of Nerium oleander Linn. Asian J Plant Sci, 3, 177-180.
  • Jamal MAHM, Rahmanb S, Islama A, Karima R, Alama S, Rahmana Z, 2012. Minimum inhibitory concentration analysis of nerium oleander against bacterial pathogens. APJTB, 2, 1664-1666.
  • Kars MD, Kars G, Gunduz U, Uney K, Bas AL, 2011. Effect of Nerium oleander distillate on MCF-7 breast cancer cell lines (Abstract). Curr Opin Biotechnol, 22, 126.
  • Ozdemir O, Ciftci MK, Maden M, 2011. Oleander poisoning in cattle. Eurasian J Vet Sci, 27, 73-76.
  • Traş B, Uney K, Traş S, 2012. Bitkisel ürünlerle ilgili sağlık sorunları. Bilim ve Teknik, 534, 48-53.
  • Yılmaz B, Emre B, Ozata İ, Şener S, 1994. Nerium Oleander’ın kobayların elektrokardiyogramına etkisi. AÜ Vet Fak Derg, 41, 389-394.

Investigation of antimicrobial effect of Nerium oleander distillate

Year 2013, Volume: 29 Issue: 3, 150 - 152, 01.09.2013

Abstract

Aim: The aim of the current study was to determine the antimicrobial effect of Nerium oleander distillate. Material and Method: Collected Nerium oleander plant was identified and authenticated. Nerium oleander was washed and chopped, later sufficient distilled water was added. The mixture was distillated by distillatory device. After the plantdistillated water mixture was started to evaporate, vapor was separated to other clean glass containers. Obtained Nerium oleander distillate was lyophilized in small glass bottle, and dry material was collected. Lyophilized dry material was dissolved at concentration of 5 mg/mL with distillated water, and its antimicrobial effect was investigated against to Escherichia coli, Salmonella Typhimurium, Salmonella Enteritidis, Staphylococcus aureus, Methicillin Resistant Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumonia, Listeria monocytogenes, Pseudomonas aeruginosa and Candida ssp. by agar well-diffusion method. Result: Nerium oleander distillate did no show any antimicrobial effect against to tested microorganism. Conclusion: It may be stated that Nerium oleander distillate has no antimicrobial effect contrary to its chemical extracts

References

  • Balabanlı C, Albayrak S, Türk M, Yüksel O, 2006. Türkiye çayır meralarında bulunan bazı zararlı bitkiler ve hayvanlar üzerindeki etkileri. SDÜ Orman Fakültesi Dergisi, 2, 89-96.
  • Bas AL, Demirci S, Yazihan N, Uney K, Kaya EE, 2012. Nerium oleander distillate improves fat and glucose metabolism in high-fat diet-fed streptozotocin-induced diabetic rats. Int J Endocrinol, 2012, 1-10.
  • Baytop T, 1984. Türkiye’de Bitkilerle Tedavi, İ. Ü. Yayınları, No: 3255, İstanbul, Türkiye, p:411.
  • Bhuvaneshwari L, Arthy E, Anitha C, Dhanabalan K, Meena M, 2007. Phytochemical analysis & antibacterial activity of Nerium oleander. Anc Sci Life, 26, 24-28.
  • Dik B, Uney K, Ozdemir O, Demirci S, Yazıhan NA, Bas AL, 2012. Acute oral toxicity of Nerium oleander distilate in rats (Abstract). J Vet Pharmacol Therap, 35, suppl 3, 78- 102.
  • Gupta V, Mittal P, 2010. Phytochemical and pharmacological potential of Nerium oleander: A review. IJPS, 1, 3.
  • Hadizadeh I, Peivastegan B, Kolahi M, 2009. Antifungal activity of nettle (Urtica diocia L.), colocynth (Citrullus colocynthis L. Schrad), oleander (Nerium oleande L.) and konar (Ziziphus spina-christi L.) extracts on plants pathogenic fungi. Pak J Biol Sci, 12, 58-63.
  • Hussain I, Khattak MUR, Ullah R, Muhammad Z, Khan N, Khan FA, Ullah Z, Haider S, 2011. Phytochemicals screening and antimicrobial activities of selected medicinal plants of Khyberpakhtunkhwa Pakistan. Afr J Pharm Pharmacol, 5, 746-750.
  • Hussain MA, Gorsi MS, 2004. Antimicrobial activity of Nerium oleander Linn. Asian J Plant Sci, 3, 177-180.
  • Jamal MAHM, Rahmanb S, Islama A, Karima R, Alama S, Rahmana Z, 2012. Minimum inhibitory concentration analysis of nerium oleander against bacterial pathogens. APJTB, 2, 1664-1666.
  • Kars MD, Kars G, Gunduz U, Uney K, Bas AL, 2011. Effect of Nerium oleander distillate on MCF-7 breast cancer cell lines (Abstract). Curr Opin Biotechnol, 22, 126.
  • Ozdemir O, Ciftci MK, Maden M, 2011. Oleander poisoning in cattle. Eurasian J Vet Sci, 27, 73-76.
  • Traş B, Uney K, Traş S, 2012. Bitkisel ürünlerle ilgili sağlık sorunları. Bilim ve Teknik, 534, 48-53.
  • Yılmaz B, Emre B, Ozata İ, Şener S, 1994. Nerium Oleander’ın kobayların elektrokardiyogramına etkisi. AÜ Vet Fak Derg, 41, 389-394.
There are 14 citations in total.

Details

Other ID JA69GB85EF
Journal Section Research
Authors

Burak Dik This is me

Zafer Sayın This is me

Orhan Çorum This is me

Publication Date September 1, 2013
Published in Issue Year 2013 Volume: 29 Issue: 3

Cite

APA Dik, B., Sayın, Z., & Çorum, O. (2013). Investigation of antimicrobial effect of Nerium oleander distillate. Eurasian Journal of Veterinary Sciences, 29(3), 150-152.
AMA Dik B, Sayın Z, Çorum O. Investigation of antimicrobial effect of Nerium oleander distillate. Eurasian J Vet Sci. September 2013;29(3):150-152.
Chicago Dik, Burak, Zafer Sayın, and Orhan Çorum. “Investigation of Antimicrobial Effect of Nerium Oleander Distillate”. Eurasian Journal of Veterinary Sciences 29, no. 3 (September 2013): 150-52.
EndNote Dik B, Sayın Z, Çorum O (September 1, 2013) Investigation of antimicrobial effect of Nerium oleander distillate. Eurasian Journal of Veterinary Sciences 29 3 150–152.
IEEE B. Dik, Z. Sayın, and O. Çorum, “Investigation of antimicrobial effect of Nerium oleander distillate”, Eurasian J Vet Sci, vol. 29, no. 3, pp. 150–152, 2013.
ISNAD Dik, Burak et al. “Investigation of Antimicrobial Effect of Nerium Oleander Distillate”. Eurasian Journal of Veterinary Sciences 29/3 (September 2013), 150-152.
JAMA Dik B, Sayın Z, Çorum O. Investigation of antimicrobial effect of Nerium oleander distillate. Eurasian J Vet Sci. 2013;29:150–152.
MLA Dik, Burak et al. “Investigation of Antimicrobial Effect of Nerium Oleander Distillate”. Eurasian Journal of Veterinary Sciences, vol. 29, no. 3, 2013, pp. 150-2.
Vancouver Dik B, Sayın Z, Çorum O. Investigation of antimicrobial effect of Nerium oleander distillate. Eurasian J Vet Sci. 2013;29(3):150-2.