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Denizli X Leghorn F2 popülasyonunda canlı ağırlık ve yumurta verimini etkileyen kromozom bölgelerinin tanımlanması

Year 2013, Volume: 29 Issue: 1, 30 - 38, 01.03.2013

Abstract

Amaç: Bu çalışmanın amacı; Denizli X Leghorn F2 populasyonunda yumurta verimi ve farklı dönemlerde canlı agırlığı kontrol eden kromozom bölgelerinin tanımlanmasıdır.Gereç ve Yöntem: Denizli ve Leghorn ırkları kullanılarak F2 düzeyinde deneysel bir populasyon oluşturuldu ve verim kayıtları alındı. Kromozom tarama çalışmaları için kantitatif özellik lokusları (QTL) gen haritalama analizlerine uygun 113 mikrosatellit markörü F0, F1 ve F2 bireylerde Polimeraz Zincir Reaksiyonu (PZR) ile yükseltgendi. Bulgular: Bu çalışmanın sonucunda farklı dönemlerde canlı ağırlık ile ilişkili QTL bölgeleri tavuk 1. kromozom çiftinde (GGA1), GGA2 ve GGA4 üzerinde tespit edildi. Yumurta verimi üzerine etkili iki farklı QTL bölgesinin varlığı, GGA8 ve cinsiyet kromozomu (GGAZ) üzerinde bulundu. GGA2, GGA4 ve GGAZ üzerinde bulunan üç farklı QTL ile yumurta ağırlığı arasında bir ilişki tespit edildi. Öneri: QTL bölgelerinin yeni markörler ile daraltılması ve bölgesel klonlama çalışmaları ile bu verim özelliklerini kontrol eden genlerin tespit edilmesi gerekmektedir.

Determination of chromosomal regions affecting body weight and egg production in Denizli X White Leghorn F2 populations

Year 2013, Volume: 29 Issue: 1, 30 - 38, 01.03.2013

Abstract

Aim: The objective of the present study was identification of the chromosomal regions responsible for egg yield and body weight at different age periods in a Denizli and White Leghorn F2 population.Materials and Methods: An experimental F2 population was constructed by crossing Denizli and White Leghorn breeds and the yields of the animals were recorded. In chromosomal scanning trials, a total of 113 microsatellite markers, suitable for use in quantitative trait locus (QTL) gene mapping, were amplified by the polymerase chain reaction (PCR) in F0, F1 and F2 animals.Results: Data obtained in the present study demonstrated that QTL regions associated with body weight at different age periods were located on chromosome 1 (GGA1), GGA2 and GGA4. It was determined that, two different QTL regions affecting egg yield existed, on GGA8 and the sex chromosome (GGAZ). Three different QTL regions located on the chromosomes GGA2, GGA4 and GGAZ were associated with egg weight.Conclusion: There is a need for narrowing these QTL regions by typing new markers in these intervals and for identifying genes that have affect on these economically important traits.

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Details

Other ID JA69HG28HC
Journal Section Research
Authors

Zafer Bulut This is me

Ercan Kurar This is me

Yusuf Ozsensoy This is me

Mehmet Nizamlioglu This is me

Mustafa Garip This is me

Alper Yilmaz This is me

Tamer Caglayan This is me

Suleyman Dere This is me

Varol Kurtoglu This is me

Muge Dogan This is me

Publication Date March 1, 2013
Published in Issue Year 2013 Volume: 29 Issue: 1

Cite

APA Bulut, Z., Kurar, E., Ozsensoy, Y., Nizamlioglu, M., et al. (2013). Determination of chromosomal regions affecting body weight and egg production in Denizli X White Leghorn F2 populations. Eurasian Journal of Veterinary Sciences, 29(1), 30-38.
AMA Bulut Z, Kurar E, Ozsensoy Y, Nizamlioglu M, Garip M, Yilmaz A, Caglayan T, Dere S, Kurtoglu V, Dogan M. Determination of chromosomal regions affecting body weight and egg production in Denizli X White Leghorn F2 populations. Eurasian J Vet Sci. March 2013;29(1):30-38.
Chicago Bulut, Zafer, Ercan Kurar, Yusuf Ozsensoy, Mehmet Nizamlioglu, Mustafa Garip, Alper Yilmaz, Tamer Caglayan, Suleyman Dere, Varol Kurtoglu, and Muge Dogan. “Determination of Chromosomal Regions Affecting Body Weight and Egg Production in Denizli X White Leghorn F2 Populations”. Eurasian Journal of Veterinary Sciences 29, no. 1 (March 2013): 30-38.
EndNote Bulut Z, Kurar E, Ozsensoy Y, Nizamlioglu M, Garip M, Yilmaz A, Caglayan T, Dere S, Kurtoglu V, Dogan M (March 1, 2013) Determination of chromosomal regions affecting body weight and egg production in Denizli X White Leghorn F2 populations. Eurasian Journal of Veterinary Sciences 29 1 30–38.
IEEE Z. Bulut, “Determination of chromosomal regions affecting body weight and egg production in Denizli X White Leghorn F2 populations”, Eurasian J Vet Sci, vol. 29, no. 1, pp. 30–38, 2013.
ISNAD Bulut, Zafer et al. “Determination of Chromosomal Regions Affecting Body Weight and Egg Production in Denizli X White Leghorn F2 Populations”. Eurasian Journal of Veterinary Sciences 29/1 (March 2013), 30-38.
JAMA Bulut Z, Kurar E, Ozsensoy Y, Nizamlioglu M, Garip M, Yilmaz A, Caglayan T, Dere S, Kurtoglu V, Dogan M. Determination of chromosomal regions affecting body weight and egg production in Denizli X White Leghorn F2 populations. Eurasian J Vet Sci. 2013;29:30–38.
MLA Bulut, Zafer et al. “Determination of Chromosomal Regions Affecting Body Weight and Egg Production in Denizli X White Leghorn F2 Populations”. Eurasian Journal of Veterinary Sciences, vol. 29, no. 1, 2013, pp. 30-38.
Vancouver Bulut Z, Kurar E, Ozsensoy Y, Nizamlioglu M, Garip M, Yilmaz A, Caglayan T, Dere S, Kurtoglu V, Dogan M. Determination of chromosomal regions affecting body weight and egg production in Denizli X White Leghorn F2 populations. Eurasian J Vet Sci. 2013;29(1):30-8.