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Transmissible venereal tümörlü dişi köpeklerde otojen aşı uygulaması

Year 2012, Volume: 28 Issue: 4, 220 - 223, 01.12.2012

Abstract

Amaç: Yapılan bu çalışmada transmissible venereal tümör (TVT) tedavisinde otojen aşı uygulamalarının başarısının belirlenmesi amaçlanmıştır.Gereç ve Yöntem: Çalışmanın hayvan materyalini 8 adet TVT’li dişi köpek oluşturdu. Köpeklere uygulanan otojen aşı bovine papillomatozis aşısı esas alınarak yapıldı. Yaklaşık 24 g tümör dokusuna 48 mL tris buffer (pH: 7.5) solüsyonu eklenerek otoklavize edilmiş steril kum yardımıyla soğuk şartlarda (+4 0C) 15-20 dakika havan içerisinde ezilerek parçalandı. Karışımın yarısı (36 mL) alınıp tris buffer ile 6065 mL’ye tamamlandı ve %0.5 oranında formalin katılıp 10 mL’lik 6 kısma ayrıldı. Hazırlanan aşı 1 hafta ara ile 4 kez (SC) uygulandı. Köpekler 4. uygulamanın ardından 3 aylık takibe alındı ve tümörün gerilemesi yönünden değerlendirmeler yapıldı. Bulgular: Köpeklerde otojen aşı uygulaması öncesinde serosanguinöz veya hemorajik genital akıntı tespit edildi. Vagina ve vulvada bulunan kitleler karnabahar biçiminde ve dokunulduğunda kolayca kanayan bir yapıda gözlendi. Otojen aşı uygulamaları sonrasında köpeklerin dış genital organlarında bulunan kitlelerden gelen serosanguinöz veya hemorajik genital akıntının kesilmediği ve kitlenin frajilitesinde bir değişiklik olmadığı tespit edildi. Otojen aşı uygulaması yapılan hayvanlarda, uygulanan 4 doz aşı sonrasında vagina ve vulvada bulunan kitlelerde herhangi bir gerileme ve iyileşmenin olmadığı belirlendi. Öneriler: TVT oluşumunda viral bir ajanın etken olabileceğine inanılmaktadır. Orijini bovine papilloma virüs olan sığırların papillomatozisinin tedavisinde başarılı sonuçlar
alınan otojen aşı uygulama tekniğinin köpeklerde TVT tedavisinde etkili olamayabileceği kanaatine varıldı.

References

  • Das U, Das K, 2000. Review of canine transmissible venereal sarcoma. Vet Res Commun, 24, 545-556.
  • Dinç DA, 1995. Meme ve meme başı derisinin lezyonları, In: Evcil hayvanlarda memenin deri hastalıkları, dolaşım bozuklukları ve operasyonları, Ed; Dinç DA, Birinci Baskı, Ülkü Matbaa, Konya, Türkiye, pp; 31-64.
  • Erer H, Kıran MN, 2000. Diğer tümörler ve neoplastik hastalıklar, In: Veteriner Onkoloji, İkinci Baskı, Damla Yayınevi, Konya, Türkiye, pp; 133-134.
  • Eze CA, Anyanwu HC, Kene ROC, 2007. Review of canine transmissible venereal tumour (TVT) in dogs. Nigerian Vet J, 28, 54-70.
  • Goldschmidt MH, Hendrick MJ, 2002. Tumors of the skin and soft tissues, In: Tumors in Domestic Animals, Ed; Meuten DJ, Iowa State Press, USA, pp; 116-117.
  • Harmelin A, Pinthus JH, Friedman-Morvinski D, Kaufman K, Brenner O, 2002. Lack of MHC expression and retention of ultrastructural characteristic by xenograft transmissible venereal tumour cells in SCID mice. Vet Immunol Immunop, 86, 245-249.
  • Jonston SD, Kustritz MVR, Olson PNS, 2001. Disorders of the canine vagina, vestibule and vulva, In: Canine and Feline Theriogenology, Ed; Kersey R, W. B. Saunders Company, Philadelphia, USA, pp: 225-242.
  • Katzir N, Rechavi G, Cohen JB, Unger T, Simoni F, Segal S, Cohen D, Givol D, 1985. ‘Retroposon’ insertion into cellular oncogene c-myc in canine transmissible venereal tumor. Proc Natl Acad Sci, 82, 1054-1058.
  • Konuk CS, Gürbulak K, Kılıçarslan MR, Gürel A, Senünver A, 2001. Treatment of transmissible venereal tumors in the bitch using the combination of triple chemotherapeutic agents. İstanbul Univ Vet Fak Derg, 27, 139-149.
  • Martins MIM, Ferreira de Souza F, Gobello C, 2005. The canine transmissible venereal tumor: Etiology, pathology, diagnosis and treatment. Recent Advances in Small Animal Reproduction, www.ivis.org, Erişim tarihi; 17.12.2010.
  • Mcentee MC, 2002. Reproductive Oncology. Clin Tech Small Anim Prac, 17, 133-149.
  • Morris J, Dobson J, 2001. Pathogenesis and Tumour Biology, In: Small Animal Oncology, Eds; Morris J, Dobson J, Blackwell Science Ltd, UK, pp; 4-14.
  • Mukaratirwa S, Gruys E, 2003. Canine transmissible venereal tumour: cytogenetic origin, immunophenotype, and immunobiology. A review. Vet Q, 25, 102-111.
  • Murgia C, Pritchard JK, Kim SY, Fassati A, Weiss RA, 2006. Clonal origin and evolution of a transmissible cancer. NIH Public Access, 126, 477-487.
  • Nak D, 2001. Dişi köpeklerde transmissible venereal tumor. YYU Vet Fak Derg, 7, 152-155. Nak D, Nak Y, Cangül İT, Tuna B, 2005. A clinico-pathological study on the effect of vincristine on transmissible venereal tumour in dogs. J Vet Med, 52, 366-370.
  • Özyurtlu N, Bademkıran S, Ünver O, Yıldız F, İçen H, 2008. Dişi bir köpekte transmissible venereal tümörün abdominal ve subkutan inguinal bölgeye metastazı. Dicle Üniv Vet Fak Derg, 1, 48-51.
  • Panchbhai VS, Karpe AG, Kulkarni GB, Kulkarni PE, 1990. Use of autogenous vaccine in transmissible canine venereal tumour. Indian Vet J, 67, 983-984.
  • Yang TJ, 1987. Parvovirus-induced regression of canine transmissible venereal sarcoma. Am J Vet Res, 48, 799- 800.

Application of autogenous vaccine in bitches with transmissible venereal tumor

Year 2012, Volume: 28 Issue: 4, 220 - 223, 01.12.2012

Abstract

Aim: The aim of the study was to determine the success of autogenous vaccine application in treatment of TVT.Materials and Methods: The study was conducted on 8 bitches with TVT. . The autogenous vaccine applied on the dogs was maddened the based on the bovine papillomatosis vaccine. Approximately 24 g of tumor tissue and 48 mL Tris buffer (pH 7.5) solution were crushed in cold conditions (+4 0C) during 15-20 minutes in mortar disintegrated with autoclaved sterile sand. Half of the mixture (36 mL) was completed with tris buffer to 60-65 mL. 0.5 percent formalin was added this mixture and mixture was portioned to 6 each has 10 mL. The prepared vaccine was administered 4 times (SC) with an interval of 1 week. Dogs were followed up 3 months after 4th application and evaluations were performed for tumor regression.Results: Serosanguineous or hemorrhagic genital discharge was determined in dogs prior to application of autogenous vaccine. The lesions in external genital organs are like cauliflower, rubescent, fresh-looking and easily bleed on touched. After administration of the autogenous vaccine, serosanguineous or hemorrhagic genital discharge from the masses on the external genital organs of dogs continued, and there were no changes in the mass fragility. After the application of autogenous vaccines at four times, there was no any decline and improvement in the masses on the vagina and vulva.Conclusions: It is believed that a viral agent may contribute to the etiology of TVT. Prepared autogenous vaccine technique used in the treatment of bovine papillomatosis derived from bovine papilloma virus may not be suitable in the treatment of bitches with TVT

References

  • Das U, Das K, 2000. Review of canine transmissible venereal sarcoma. Vet Res Commun, 24, 545-556.
  • Dinç DA, 1995. Meme ve meme başı derisinin lezyonları, In: Evcil hayvanlarda memenin deri hastalıkları, dolaşım bozuklukları ve operasyonları, Ed; Dinç DA, Birinci Baskı, Ülkü Matbaa, Konya, Türkiye, pp; 31-64.
  • Erer H, Kıran MN, 2000. Diğer tümörler ve neoplastik hastalıklar, In: Veteriner Onkoloji, İkinci Baskı, Damla Yayınevi, Konya, Türkiye, pp; 133-134.
  • Eze CA, Anyanwu HC, Kene ROC, 2007. Review of canine transmissible venereal tumour (TVT) in dogs. Nigerian Vet J, 28, 54-70.
  • Goldschmidt MH, Hendrick MJ, 2002. Tumors of the skin and soft tissues, In: Tumors in Domestic Animals, Ed; Meuten DJ, Iowa State Press, USA, pp; 116-117.
  • Harmelin A, Pinthus JH, Friedman-Morvinski D, Kaufman K, Brenner O, 2002. Lack of MHC expression and retention of ultrastructural characteristic by xenograft transmissible venereal tumour cells in SCID mice. Vet Immunol Immunop, 86, 245-249.
  • Jonston SD, Kustritz MVR, Olson PNS, 2001. Disorders of the canine vagina, vestibule and vulva, In: Canine and Feline Theriogenology, Ed; Kersey R, W. B. Saunders Company, Philadelphia, USA, pp: 225-242.
  • Katzir N, Rechavi G, Cohen JB, Unger T, Simoni F, Segal S, Cohen D, Givol D, 1985. ‘Retroposon’ insertion into cellular oncogene c-myc in canine transmissible venereal tumor. Proc Natl Acad Sci, 82, 1054-1058.
  • Konuk CS, Gürbulak K, Kılıçarslan MR, Gürel A, Senünver A, 2001. Treatment of transmissible venereal tumors in the bitch using the combination of triple chemotherapeutic agents. İstanbul Univ Vet Fak Derg, 27, 139-149.
  • Martins MIM, Ferreira de Souza F, Gobello C, 2005. The canine transmissible venereal tumor: Etiology, pathology, diagnosis and treatment. Recent Advances in Small Animal Reproduction, www.ivis.org, Erişim tarihi; 17.12.2010.
  • Mcentee MC, 2002. Reproductive Oncology. Clin Tech Small Anim Prac, 17, 133-149.
  • Morris J, Dobson J, 2001. Pathogenesis and Tumour Biology, In: Small Animal Oncology, Eds; Morris J, Dobson J, Blackwell Science Ltd, UK, pp; 4-14.
  • Mukaratirwa S, Gruys E, 2003. Canine transmissible venereal tumour: cytogenetic origin, immunophenotype, and immunobiology. A review. Vet Q, 25, 102-111.
  • Murgia C, Pritchard JK, Kim SY, Fassati A, Weiss RA, 2006. Clonal origin and evolution of a transmissible cancer. NIH Public Access, 126, 477-487.
  • Nak D, 2001. Dişi köpeklerde transmissible venereal tumor. YYU Vet Fak Derg, 7, 152-155. Nak D, Nak Y, Cangül İT, Tuna B, 2005. A clinico-pathological study on the effect of vincristine on transmissible venereal tumour in dogs. J Vet Med, 52, 366-370.
  • Özyurtlu N, Bademkıran S, Ünver O, Yıldız F, İçen H, 2008. Dişi bir köpekte transmissible venereal tümörün abdominal ve subkutan inguinal bölgeye metastazı. Dicle Üniv Vet Fak Derg, 1, 48-51.
  • Panchbhai VS, Karpe AG, Kulkarni GB, Kulkarni PE, 1990. Use of autogenous vaccine in transmissible canine venereal tumour. Indian Vet J, 67, 983-984.
  • Yang TJ, 1987. Parvovirus-induced regression of canine transmissible venereal sarcoma. Am J Vet Res, 48, 799- 800.
There are 18 citations in total.

Details

Other ID JA69HS53KS
Journal Section Research
Authors

Sakine Ülküm Çizmeci This is me

Ayşe Merve Köse This is me

İbrahim Aydın This is me

Dursun Ali Dinç This is me

Mehmet Maden This is me

Serkan İrfan Köse This is me

Publication Date December 1, 2012
Published in Issue Year 2012 Volume: 28 Issue: 4

Cite

APA Çizmeci, S. Ü., Köse, A. M., Aydın, İ., Dinç, D. A., et al. (2012). Application of autogenous vaccine in bitches with transmissible venereal tumor. Eurasian Journal of Veterinary Sciences, 28(4), 220-223.
AMA Çizmeci SÜ, Köse AM, Aydın İ, Dinç DA, Maden M, Köse Sİ. Application of autogenous vaccine in bitches with transmissible venereal tumor. Eurasian J Vet Sci. December 2012;28(4):220-223.
Chicago Çizmeci, Sakine Ülküm, Ayşe Merve Köse, İbrahim Aydın, Dursun Ali Dinç, Mehmet Maden, and Serkan İrfan Köse. “Application of Autogenous Vaccine in Bitches With Transmissible Venereal Tumor”. Eurasian Journal of Veterinary Sciences 28, no. 4 (December 2012): 220-23.
EndNote Çizmeci SÜ, Köse AM, Aydın İ, Dinç DA, Maden M, Köse Sİ (December 1, 2012) Application of autogenous vaccine in bitches with transmissible venereal tumor. Eurasian Journal of Veterinary Sciences 28 4 220–223.
IEEE S. Ü. Çizmeci, A. M. Köse, İ. Aydın, D. A. Dinç, M. Maden, and S. İ. Köse, “Application of autogenous vaccine in bitches with transmissible venereal tumor”, Eurasian J Vet Sci, vol. 28, no. 4, pp. 220–223, 2012.
ISNAD Çizmeci, Sakine Ülküm et al. “Application of Autogenous Vaccine in Bitches With Transmissible Venereal Tumor”. Eurasian Journal of Veterinary Sciences 28/4 (December 2012), 220-223.
JAMA Çizmeci SÜ, Köse AM, Aydın İ, Dinç DA, Maden M, Köse Sİ. Application of autogenous vaccine in bitches with transmissible venereal tumor. Eurasian J Vet Sci. 2012;28:220–223.
MLA Çizmeci, Sakine Ülküm et al. “Application of Autogenous Vaccine in Bitches With Transmissible Venereal Tumor”. Eurasian Journal of Veterinary Sciences, vol. 28, no. 4, 2012, pp. 220-3.
Vancouver Çizmeci SÜ, Köse AM, Aydın İ, Dinç DA, Maden M, Köse Sİ. Application of autogenous vaccine in bitches with transmissible venereal tumor. Eurasian J Vet Sci. 2012;28(4):220-3.