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Tavuklarda mikoplazmozisin patolojik ve Real Time-PCR metotları ile teşhisi

Year 2012, Volume: 28 Issue: 2, 82 - 86, 01.06.2012

Abstract

Amaç: Bu çalışmanın amacı tavuklarda mikoplazmozisin teşhisinde Real Time-PCR’ın kullanılabilirliğini araştırmak ve mikoplazma tespit edilen tavuklardan alınan akciğer, hava keseleri, trake, kalp, karaciğer ve böbrek dokularındaki patolojik bulguları belirlemektir.Gereç ve Yöntem: Solunum problemi şikayeti olan 3 ayrı işletmeye ait 3 kümesten, hastalık bulgusu gösteren 10’ar adet broyler piliçten toplanan konjuktival ve trakel svap örnekleri ile aynı piliçlerden nekropsiyi takiben alınan trake, hava kesesi, akciğer, karaciğer, böbrek ve kalp dokuları patolojik, Real Time-PCR (RT-PCR) ve mikrobiyolojik metotlarla incelenmiştir. Bulgular: Çalışmada, klinik olarak burun ve gözyaşı akıntısı ile hırıltılı solunum, makroskobik olarak trake ve bronşlarda kataral eksudat görüldü. Mikroskobik olarak trake ve bronş epitellerinde ve goblet hücrelerinde hiperplazi ile lamina propriada mononükleer hücre infiltrasyonlarının şekillendiği belirlendi. Sekiz broyler pilicin trake dokusundan Mycoplasma ssp. izolasyonu yapıldı. RT-PCR ile 22 broylere ait doku ve svap örneklerinde Mycoplasma gallisepticum nükleik asitleri amplifiye edildi. Öneri: Tavuklarda mikoplazmozisin teşhisinde, etken izolasyonunun geciktiği ya da yapılamadığı durumlarda RTPCR’nin önemli bir alternatif olabileceği kanısına varılmıştır.

References

  • Callison SA, Riblet SM, Sun S, Ikuta N, Hilt D, Leiting V, Kleven SH, Suarez DL, Garcia M, 2006. Development and validation of a real-time Taqman polymerase chain reaction assay for the detection of M. gallisepticum in naturally infected birds. Avian Dis, 50, 537-544.
  • Çarlı KT, Eyigör A, 2003. Real-time polymerase chain reaction for detection of M. gallisepticum in chicken trachea. Avian Dis, 47, 712-717.
  • Garcia M, Ikuta N, Levisohn S, Kleven SH, 2005. Evaluation and comparison of various PCR methods for detection of M. gallisepticum infection in chickens. Avian Dis, 49, 125-132.
  • Gaunson JE, Philip C J, Whithear KG, Browning GF, 2000. Lymphocytic infiltration in the chicken trachea in response to M. gallisepticum infection. Microbiology, 146, 1223-1229.
  • Grodio JL, Priscilla HD, Schat KA, 2008. Detection and quantification of M. gallisepticum genome load experimentally infected house finches (Copradacus mexicanus) using real-time polymerase chain reaction. Avian Pathol, 37, 385-391.
  • Levisohn S, Kleven SH, 2000. Avian mycoplasmosis (Mycoplasma gallisepticum). Rev Sci Tech, 19, 425-442.
  • Levisohn S, Dykstra MJ, Lin MY, Kleven SH, 1986. Comparison of in vivo and in vitro methods for pathogenicity evaluation for Mycoplasma gallisepticum in respiratory infection. Avian Pathol, 15, 233-246.
  • Ley DH, 2003. Mycoplasma gallisepticum infection, In: Diseases of Poultry, Eds; Calnek BE, Barnes HJ, Beard CW, McDougald LR, Saif YM, 11th edition, Ames, Iowa, Iowa State University Press, USA, pp: 194-207
  • Mekkes DR, Feberwee A, 2005. Real-time polymerase chain reaction for the qualitative and quantitative detection of Mycoplasma gallisepticum. Avian Pathol, 34, 348-354.
  • Nascimento ER, Yamamoto R, Herrick RK, Tait RC, 1991. Polymerase chain reaction for detection of Mycoplasma gallisepticum. Avian Dis, 35, 62-69.
  • Nunoya T, Tajima M, Yagihashi T, Sannai S, 1987. Evaluation of respiratory lesions in chikens induced by M. gallisepticum. Jpn J Vet Sci, 49, 621-629.
  • Özdemir Ö, Erer H, 2008. Pathological and microbiological investigations on the lesions of the respiratory systems of laying hens. Eurasian J Vet Sci, 24,2,55-68.
  • Papazisi L, Frasca SJR, Gladd M, Liao X, Yogev D, Geary SJ, 2002. GapA and CrmA coexpression is essential for Mycoplasma gallisepticum cytadherence and virulence. Infect Immun, 70, 6839-6845.
  • Rodriguez R, Kleven SH, 1980. Pathogenicity of two strains of Mycoplasma gallisepticum in broilers. Avian Dis, 24, 800-807.
  • Türkaslan J, Salihoğlu H, 1989. Çeşitli besiyerleri kullanılarak Mycoplasma gallisepticum’un bakteriyolojik yöntemlerle izolasyon ve identifikasyonu. Pendik Hay Hast Araşt Enst Derg, 20, 53-59.
  • Zanella A, 2007. Poultry Disease Manual, FATRO, Italy.

Diagnosis of mycoplasmosis in chicks by pathological and Real Time-PCR methods

Year 2012, Volume: 28 Issue: 2, 82 - 86, 01.06.2012

Abstract

Aim: The purpose of this study was to investigate the suitability of the Real Time-PCR in the diagnosis of mycoplasmosis and to determine the pathologic findings in lungs, air sacs, trachea, hearth, liver and kidney tissues. Materials and Methods: Conjunctiva and tracheal swab samples were taken from broiler chicks with respiratory disease complaints from 3 different breeders were used. Ten chicks from three separate flock in each breeders were collected. Trachea, air sac, lung, liver, kidney, and heart samples were also collected from the same chicks after necropsy in order to perform pathological, microbiological and Real Time-PCR analyses. Results: Clinically, nasal and conjunctival discharge and wheezing were observed. Macroscopic examination illustrated gross catarrhal exudation in trachea and bronchus. In microscopically, hyperplasia in trachea and bronchus epithelia, mucus producing cells and mononuclear cellular infiltration in lamina propria were observed. Mycoplasma spp. were successfully isolated in the tracheal tissue of 8 broiler chicks. M. gallisepticum specific nucleic acid was amplified from tissue and swab samples of 22 broiler chicks by RT-PCR. Conclusion: RT-PCR seems to be an alternative method when microbiological analyses are laborious or fails in diagnosis of mycoplasmosis.

References

  • Callison SA, Riblet SM, Sun S, Ikuta N, Hilt D, Leiting V, Kleven SH, Suarez DL, Garcia M, 2006. Development and validation of a real-time Taqman polymerase chain reaction assay for the detection of M. gallisepticum in naturally infected birds. Avian Dis, 50, 537-544.
  • Çarlı KT, Eyigör A, 2003. Real-time polymerase chain reaction for detection of M. gallisepticum in chicken trachea. Avian Dis, 47, 712-717.
  • Garcia M, Ikuta N, Levisohn S, Kleven SH, 2005. Evaluation and comparison of various PCR methods for detection of M. gallisepticum infection in chickens. Avian Dis, 49, 125-132.
  • Gaunson JE, Philip C J, Whithear KG, Browning GF, 2000. Lymphocytic infiltration in the chicken trachea in response to M. gallisepticum infection. Microbiology, 146, 1223-1229.
  • Grodio JL, Priscilla HD, Schat KA, 2008. Detection and quantification of M. gallisepticum genome load experimentally infected house finches (Copradacus mexicanus) using real-time polymerase chain reaction. Avian Pathol, 37, 385-391.
  • Levisohn S, Kleven SH, 2000. Avian mycoplasmosis (Mycoplasma gallisepticum). Rev Sci Tech, 19, 425-442.
  • Levisohn S, Dykstra MJ, Lin MY, Kleven SH, 1986. Comparison of in vivo and in vitro methods for pathogenicity evaluation for Mycoplasma gallisepticum in respiratory infection. Avian Pathol, 15, 233-246.
  • Ley DH, 2003. Mycoplasma gallisepticum infection, In: Diseases of Poultry, Eds; Calnek BE, Barnes HJ, Beard CW, McDougald LR, Saif YM, 11th edition, Ames, Iowa, Iowa State University Press, USA, pp: 194-207
  • Mekkes DR, Feberwee A, 2005. Real-time polymerase chain reaction for the qualitative and quantitative detection of Mycoplasma gallisepticum. Avian Pathol, 34, 348-354.
  • Nascimento ER, Yamamoto R, Herrick RK, Tait RC, 1991. Polymerase chain reaction for detection of Mycoplasma gallisepticum. Avian Dis, 35, 62-69.
  • Nunoya T, Tajima M, Yagihashi T, Sannai S, 1987. Evaluation of respiratory lesions in chikens induced by M. gallisepticum. Jpn J Vet Sci, 49, 621-629.
  • Özdemir Ö, Erer H, 2008. Pathological and microbiological investigations on the lesions of the respiratory systems of laying hens. Eurasian J Vet Sci, 24,2,55-68.
  • Papazisi L, Frasca SJR, Gladd M, Liao X, Yogev D, Geary SJ, 2002. GapA and CrmA coexpression is essential for Mycoplasma gallisepticum cytadherence and virulence. Infect Immun, 70, 6839-6845.
  • Rodriguez R, Kleven SH, 1980. Pathogenicity of two strains of Mycoplasma gallisepticum in broilers. Avian Dis, 24, 800-807.
  • Türkaslan J, Salihoğlu H, 1989. Çeşitli besiyerleri kullanılarak Mycoplasma gallisepticum’un bakteriyolojik yöntemlerle izolasyon ve identifikasyonu. Pendik Hay Hast Araşt Enst Derg, 20, 53-59.
  • Zanella A, 2007. Poultry Disease Manual, FATRO, Italy.
There are 16 citations in total.

Details

Other ID JA69KC92ZA
Journal Section Research
Authors

Mehmet Tuzcu This is me

Murat Özmen This is me

S. Reyhan Karakoç This is me

Nevin Tuzcu This is me

Atila Yoldaş This is me

Publication Date June 1, 2012
Published in Issue Year 2012 Volume: 28 Issue: 2

Cite

APA Tuzcu, M., Özmen, M., Karakoç, S. R., Tuzcu, N., et al. (2012). Diagnosis of mycoplasmosis in chicks by pathological and Real Time-PCR methods. Eurasian Journal of Veterinary Sciences, 28(2), 82-86.
AMA Tuzcu M, Özmen M, Karakoç SR, Tuzcu N, Yoldaş A. Diagnosis of mycoplasmosis in chicks by pathological and Real Time-PCR methods. Eurasian J Vet Sci. June 2012;28(2):82-86.
Chicago Tuzcu, Mehmet, Murat Özmen, S. Reyhan Karakoç, Nevin Tuzcu, and Atila Yoldaş. “Diagnosis of Mycoplasmosis in Chicks by Pathological and Real Time-PCR Methods”. Eurasian Journal of Veterinary Sciences 28, no. 2 (June 2012): 82-86.
EndNote Tuzcu M, Özmen M, Karakoç SR, Tuzcu N, Yoldaş A (June 1, 2012) Diagnosis of mycoplasmosis in chicks by pathological and Real Time-PCR methods. Eurasian Journal of Veterinary Sciences 28 2 82–86.
IEEE M. Tuzcu, M. Özmen, S. R. Karakoç, N. Tuzcu, and A. Yoldaş, “Diagnosis of mycoplasmosis in chicks by pathological and Real Time-PCR methods”, Eurasian J Vet Sci, vol. 28, no. 2, pp. 82–86, 2012.
ISNAD Tuzcu, Mehmet et al. “Diagnosis of Mycoplasmosis in Chicks by Pathological and Real Time-PCR Methods”. Eurasian Journal of Veterinary Sciences 28/2 (June 2012), 82-86.
JAMA Tuzcu M, Özmen M, Karakoç SR, Tuzcu N, Yoldaş A. Diagnosis of mycoplasmosis in chicks by pathological and Real Time-PCR methods. Eurasian J Vet Sci. 2012;28:82–86.
MLA Tuzcu, Mehmet et al. “Diagnosis of Mycoplasmosis in Chicks by Pathological and Real Time-PCR Methods”. Eurasian Journal of Veterinary Sciences, vol. 28, no. 2, 2012, pp. 82-86.
Vancouver Tuzcu M, Özmen M, Karakoç SR, Tuzcu N, Yoldaş A. Diagnosis of mycoplasmosis in chicks by pathological and Real Time-PCR methods. Eurasian J Vet Sci. 2012;28(2):82-6.